TY  - JOUR
AU  - Mićanović, Dragica
AU  - Stanisavljević, Suzana
AU  - Li, Hanluo
AU  - Koprivica, Ivan
AU  - Jonić, Natalija
AU  - Stojanović, Ivana D.
AU  - Savković, Vuk
AU  - Saksida, Tamara
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6829
AB  - Mesenchymal stem cells (MSCs) are known for their immunosuppressive properties.
Based on the demonstrated anti-inflammatory effect of mouse MSCs from hair follicles (moMSCORS) in a murine wound closure model, this study evaluates their potential for preventing type
1 diabetes (T1D) in C57BL/6 mice. T1D was induced in C57BL/6 mice by repeated low doses of streptozotocin. moMSCORS were injected intravenously on weekly basis. moMSCORS reduced T1D incidence, the insulitis stage, and preserved insulin production in treated animals. moMSCORS primarily exerted immunomodulatory effects by inhibiting CD4+ T cell proliferation and activation. Ex
vivo analysis indicated that moMSCORS modified the cellular immune profile within pancreatic
lymph nodes and pancreatic infiltrates by reducing the numbers of M1 pro-inflammatory macrophages and T helper 17 cells and upscaling the immunosuppressive T regulatory cells. The proportion of pathogenic insulin-specific CD4+ T cells was down-scaled in the lymph nodes, likely via soluble factors. The moMSCORS detected in the pancreatic infiltrates of treated mice presumably exerted the observed suppressive effect on CD4+ through direct contact. moMSCORS alleviated T1D
symptoms in the mouse, qualifying as a candidate for therapeutic products by multiple advantages:
non-invasive sampling by epilation, easy access, permanent availability, scalability, and benefits of
auto-transplantation.
PB  - Basel: MDPI
T2  - International Journal of Molecular Sciences
T1  - Mesenchymal stem cells from mouse hair follicles Inhibit the development of type 1 diabetes
IS  - 11
VL  - 25
DO  - 10.3390/ijms25115974
SP  - 5974
ER  - 

@article{
author = "Mićanović, Dragica and Stanisavljević, Suzana and Li, Hanluo and Koprivica, Ivan and Jonić, Natalija and Stojanović, Ivana D. and Savković, Vuk and Saksida, Tamara",
year = "2024",
abstract = "Mesenchymal stem cells (MSCs) are known for their immunosuppressive properties.
Based on the demonstrated anti-inflammatory effect of mouse MSCs from hair follicles (moMSCORS) in a murine wound closure model, this study evaluates their potential for preventing type
1 diabetes (T1D) in C57BL/6 mice. T1D was induced in C57BL/6 mice by repeated low doses of streptozotocin. moMSCORS were injected intravenously on weekly basis. moMSCORS reduced T1D incidence, the insulitis stage, and preserved insulin production in treated animals. moMSCORS primarily exerted immunomodulatory effects by inhibiting CD4+ T cell proliferation and activation. Ex
vivo analysis indicated that moMSCORS modified the cellular immune profile within pancreatic
lymph nodes and pancreatic infiltrates by reducing the numbers of M1 pro-inflammatory macrophages and T helper 17 cells and upscaling the immunosuppressive T regulatory cells. The proportion of pathogenic insulin-specific CD4+ T cells was down-scaled in the lymph nodes, likely via soluble factors. The moMSCORS detected in the pancreatic infiltrates of treated mice presumably exerted the observed suppressive effect on CD4+ through direct contact. moMSCORS alleviated T1D
symptoms in the mouse, qualifying as a candidate for therapeutic products by multiple advantages:
non-invasive sampling by epilation, easy access, permanent availability, scalability, and benefits of
auto-transplantation.",
publisher = "Basel: MDPI",
journal = "International Journal of Molecular Sciences",
title = "Mesenchymal stem cells from mouse hair follicles Inhibit the development of type 1 diabetes",
number = "11",
volume = "25",
doi = "10.3390/ijms25115974",
pages = "5974"
}

Mićanović, D., Stanisavljević, S., Li, H., Koprivica, I., Jonić, N., Stojanović, I. D., Savković, V.,& Saksida, T.. (2024). Mesenchymal stem cells from mouse hair follicles Inhibit the development of type 1 diabetes. in International Journal of Molecular Sciences
Basel: MDPI., 25(11), 5974.
https://doi.org/10.3390/ijms25115974

Mićanović D, Stanisavljević S, Li H, Koprivica I, Jonić N, Stojanović ID, Savković V, Saksida T. Mesenchymal stem cells from mouse hair follicles Inhibit the development of type 1 diabetes. in International Journal of Molecular Sciences. 2024;25(11):5974.
doi:10.3390/ijms25115974 .

Mićanović, Dragica, Stanisavljević, Suzana, Li, Hanluo, Koprivica, Ivan, Jonić, Natalija, Stojanović, Ivana D., Savković, Vuk, Saksida, Tamara, "Mesenchymal stem cells from mouse hair follicles Inhibit the development of type 1 diabetes" in International Journal of Molecular Sciences, 25, no. 11 (2024):5974,
https://doi.org/10.3390/ijms25115974 . .

TY  - JOUR
AU  - Pavelegini de Medeiros, Jaqueline
AU  - Alves Rodrigues, Selma
AU  - Sakumoto, Karina
AU  - Ruiz, Suelen Pereira
AU  - Faria, Maria Graciela Iecher
AU  - Gonçalves, José Eduardo
AU  - Junior, Ranulfo Piau
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
AU  - Dib Gonçalves, Daniela
AU  - Mandim, Filipa
AU  - Barros, Lillian
AU  - Gazim, Zilda Cristiani
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6828
AB  - Introduction: Lung cancer is the most commonly diagnosed and the main cause of cancer death, usually related to cigarette smoking. Furthermore, the microbiota of people exposed to cigarette smoke can be modified, making it difficult to eliminate opportunistic microorganisms. The leaves of Eugenia pyriformis are a by-product of fruit production and, to date, there have been no studies addressing the antiproliferative, anti-inflammatory, and antimicrobial activities.
Objective: Investigate the antimicrobial, Nitric Oxide (NO)-production inhibition, and antiproliferative activities of the essential oil from E. pyriformis leaves and its possible effect on the treatment and prevention of damage caused by tobacco.
Methods: The essential oil (EO) was obtained by hydrodistillation (3 h). Its chemical composition was investigated by GC-MS. It was proposed to investigate antiproliferative activity against human tumor cell lines, namely, breast adenocarcinoma (MCF-7), lung (NCI-H460), cervical (HeLa), and hepatocellular (HepG2) carcinomas. A non-tumor primary culture from pig liver (PLP2) was also tested. The EO capacity to inhibit nitric oxide (NO) production was evaluated by a lipopolysaccharide stimulated murine macrophage cell line. Antibacterial and antifungal activities against opportunistic pathogens were investigated against seven strains of bacteria and eight fungi.
Results: The results indicated the presence of 23 compounds in the essential oil, the majority were spathulenol (45.63%) and β-caryophyllene oxide (12.72%). Leaf EO provided 50% inhibition of nitric oxide production at a concentration of 92.04 µg mL-1. The EO also demonstrated antiproliferative activity against all human tumor cell lines studied, with GI50 values comprised between 270.86 and 337.25 µg mL−1. The essential oil showed antimicrobial potential against the bacteria Listeria monocytogenes (Murray et al.) Pirie (NCTC 7973) and Salmonella Typhimurium ATCC 13311 (MIC 1870 µg mL−1) and fungi Aspergillus versicolor ATCC 11730, Aspergillus ochraceus ATCC 12066, Penicillium ochrochloron ATCC 90288, Penicillium verrucosum var. cyclopium (Westling) Samson, Stolk & Hadlok (food isolate) (MIC 1870 µg mL−1) and Trichoderma viride Pers. IAM 5061 (1,400 µg mL–1).
Conclusion: The demonstrated anti-inflammatory, antiproliferative, and antimicrobial activities in the leaves of E. pyriformis can add value to the production chain of this plant, being a possible option for preventing and combating cancer, including lung cancer.
PB  - Lausanne: Frontiers Media SA
T2  - Frontiers Pharmacology
T1  - Bioactives of the essential oil from the leaves of Eugenia pyriformis Cambess (Myrtaceae) on the effects of tobacco
VL  - 15
DO  - 10.3389/fphar.2024.1415659
SP  - 1415659
ER  - 

@article{
author = "Pavelegini de Medeiros, Jaqueline and Alves Rodrigues, Selma and Sakumoto, Karina and Ruiz, Suelen Pereira and Faria, Maria Graciela Iecher and Gonçalves, José Eduardo and Junior, Ranulfo Piau and Glamočlija, Jasmina and Soković, Marina and Dib Gonçalves, Daniela and Mandim, Filipa and Barros, Lillian and Gazim, Zilda Cristiani",
year = "2024",
abstract = "Introduction: Lung cancer is the most commonly diagnosed and the main cause of cancer death, usually related to cigarette smoking. Furthermore, the microbiota of people exposed to cigarette smoke can be modified, making it difficult to eliminate opportunistic microorganisms. The leaves of Eugenia pyriformis are a by-product of fruit production and, to date, there have been no studies addressing the antiproliferative, anti-inflammatory, and antimicrobial activities.
Objective: Investigate the antimicrobial, Nitric Oxide (NO)-production inhibition, and antiproliferative activities of the essential oil from E. pyriformis leaves and its possible effect on the treatment and prevention of damage caused by tobacco.
Methods: The essential oil (EO) was obtained by hydrodistillation (3 h). Its chemical composition was investigated by GC-MS. It was proposed to investigate antiproliferative activity against human tumor cell lines, namely, breast adenocarcinoma (MCF-7), lung (NCI-H460), cervical (HeLa), and hepatocellular (HepG2) carcinomas. A non-tumor primary culture from pig liver (PLP2) was also tested. The EO capacity to inhibit nitric oxide (NO) production was evaluated by a lipopolysaccharide stimulated murine macrophage cell line. Antibacterial and antifungal activities against opportunistic pathogens were investigated against seven strains of bacteria and eight fungi.
Results: The results indicated the presence of 23 compounds in the essential oil, the majority were spathulenol (45.63%) and β-caryophyllene oxide (12.72%). Leaf EO provided 50% inhibition of nitric oxide production at a concentration of 92.04 µg mL-1. The EO also demonstrated antiproliferative activity against all human tumor cell lines studied, with GI50 values comprised between 270.86 and 337.25 µg mL−1. The essential oil showed antimicrobial potential against the bacteria Listeria monocytogenes (Murray et al.) Pirie (NCTC 7973) and Salmonella Typhimurium ATCC 13311 (MIC 1870 µg mL−1) and fungi Aspergillus versicolor ATCC 11730, Aspergillus ochraceus ATCC 12066, Penicillium ochrochloron ATCC 90288, Penicillium verrucosum var. cyclopium (Westling) Samson, Stolk & Hadlok (food isolate) (MIC 1870 µg mL−1) and Trichoderma viride Pers. IAM 5061 (1,400 µg mL–1).
Conclusion: The demonstrated anti-inflammatory, antiproliferative, and antimicrobial activities in the leaves of E. pyriformis can add value to the production chain of this plant, being a possible option for preventing and combating cancer, including lung cancer.",
publisher = "Lausanne: Frontiers Media SA",
journal = "Frontiers Pharmacology",
title = "Bioactives of the essential oil from the leaves of Eugenia pyriformis Cambess (Myrtaceae) on the effects of tobacco",
volume = "15",
doi = "10.3389/fphar.2024.1415659",
pages = "1415659"
}

Pavelegini de Medeiros, J., Alves Rodrigues, S., Sakumoto, K., Ruiz, S. P., Faria, M. G. I., Gonçalves, J. E., Junior, R. P., Glamočlija, J., Soković, M., Dib Gonçalves, D., Mandim, F., Barros, L.,& Gazim, Z. C.. (2024). Bioactives of the essential oil from the leaves of Eugenia pyriformis Cambess (Myrtaceae) on the effects of tobacco. in Frontiers Pharmacology
Lausanne: Frontiers Media SA., 15, 1415659.
https://doi.org/10.3389/fphar.2024.1415659

Pavelegini de Medeiros J, Alves Rodrigues S, Sakumoto K, Ruiz SP, Faria MGI, Gonçalves JE, Junior RP, Glamočlija J, Soković M, Dib Gonçalves D, Mandim F, Barros L, Gazim ZC. Bioactives of the essential oil from the leaves of Eugenia pyriformis Cambess (Myrtaceae) on the effects of tobacco. in Frontiers Pharmacology. 2024;15:1415659.
doi:10.3389/fphar.2024.1415659 .

Pavelegini de Medeiros, Jaqueline, Alves Rodrigues, Selma, Sakumoto, Karina, Ruiz, Suelen Pereira, Faria, Maria Graciela Iecher, Gonçalves, José Eduardo, Junior, Ranulfo Piau, Glamočlija, Jasmina, Soković, Marina, Dib Gonçalves, Daniela, Mandim, Filipa, Barros, Lillian, Gazim, Zilda Cristiani, "Bioactives of the essential oil from the leaves of Eugenia pyriformis Cambess (Myrtaceae) on the effects of tobacco" in Frontiers Pharmacology, 15 (2024):1415659,
https://doi.org/10.3389/fphar.2024.1415659 . .

TY  - JOUR
AU  - Ninković, Slavica
AU  - Motyka, Václav
AU  - Stanišić, Mariana
AU  - Smailagić, Dijana
AU  - Živanović, Branka
AU  - Dobrev, Petre I.
AU  - Banjac, Nevena
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6826
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6827
AB  - Compounds exuded from roots play a key role in regulating plant allelopathic interactions. However, phytochormone profiling of root exudates and their contribution to an overall allelochemical activity of specific plant species is neglected topic in allelochemical research.  Hairy root growth media of two different species, the fruit tree species  Malus × domestica Borkh. and the herbaceous weed species Chenopodium murale L. were collected and analyzed by high-performance liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). We found that most of the phytohormones exuded by the hairy roots of C. murale and M. domestica were associated with the acidic fraction (96.8% and 98.9%, respectively), including 2-oxindole-3-acetic acid, phenylacetic acid, salicylic acid (SA), benzoic acid (BzA), and abscisic acid, with SA and BzA being the most abundant, while those associated with the basic fraction, including cytokinins and the ethylene precursor 1-aminocyclopropane-1-carboxylic acid, accounted for only 1% of the plant growth substances detected in both species. Exogenous application of 0.2 µM SA, which was released from the hairy roots of C. murale and accumulated in the culture media for four weeks, significantly impaired hairy root growth of M. domestica and also shoot and root growth of Arabidopsis seedlings. The disruptive effect of 0.2 µM SA on the membrane potential of M. domestica hairy root and Arabidopsis root cells was determined. The data obtained could be useful for planning further studies aimed at clarifying the contribution and role of exuded phytohormones to the overall allelopathic potential of these two plant species.
PB  - New York: Springer
T2  - Journal of Plant Growth Regulation
T1  - Phytohormone profiling of Malus domestica and Chenopodium murale hairy root exudate - Association with Allelopathic Effects
DO  - 10.1007/s00344-024-11328-5
ER  - 

@article{
author = "Ninković, Slavica and Motyka, Václav and Stanišić, Mariana and Smailagić, Dijana and Živanović, Branka and Dobrev, Petre I. and Banjac, Nevena",
year = "2024",
abstract = "Compounds exuded from roots play a key role in regulating plant allelopathic interactions. However, phytochormone profiling of root exudates and their contribution to an overall allelochemical activity of specific plant species is neglected topic in allelochemical research.  Hairy root growth media of two different species, the fruit tree species  Malus × domestica Borkh. and the herbaceous weed species Chenopodium murale L. were collected and analyzed by high-performance liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). We found that most of the phytohormones exuded by the hairy roots of C. murale and M. domestica were associated with the acidic fraction (96.8% and 98.9%, respectively), including 2-oxindole-3-acetic acid, phenylacetic acid, salicylic acid (SA), benzoic acid (BzA), and abscisic acid, with SA and BzA being the most abundant, while those associated with the basic fraction, including cytokinins and the ethylene precursor 1-aminocyclopropane-1-carboxylic acid, accounted for only 1% of the plant growth substances detected in both species. Exogenous application of 0.2 µM SA, which was released from the hairy roots of C. murale and accumulated in the culture media for four weeks, significantly impaired hairy root growth of M. domestica and also shoot and root growth of Arabidopsis seedlings. The disruptive effect of 0.2 µM SA on the membrane potential of M. domestica hairy root and Arabidopsis root cells was determined. The data obtained could be useful for planning further studies aimed at clarifying the contribution and role of exuded phytohormones to the overall allelopathic potential of these two plant species.",
publisher = "New York: Springer",
journal = "Journal of Plant Growth Regulation",
title = "Phytohormone profiling of Malus domestica and Chenopodium murale hairy root exudate - Association with Allelopathic Effects",
doi = "10.1007/s00344-024-11328-5"
}

Ninković, S., Motyka, V., Stanišić, M., Smailagić, D., Živanović, B., Dobrev, P. I.,& Banjac, N.. (2024). Phytohormone profiling of Malus domestica and Chenopodium murale hairy root exudate - Association with Allelopathic Effects. in Journal of Plant Growth Regulation
New York: Springer..
https://doi.org/10.1007/s00344-024-11328-5

Ninković S, Motyka V, Stanišić M, Smailagić D, Živanović B, Dobrev PI, Banjac N. Phytohormone profiling of Malus domestica and Chenopodium murale hairy root exudate - Association with Allelopathic Effects. in Journal of Plant Growth Regulation. 2024;.
doi:10.1007/s00344-024-11328-5 .

Ninković, Slavica, Motyka, Václav, Stanišić, Mariana, Smailagić, Dijana, Živanović, Branka, Dobrev, Petre I., Banjac, Nevena, "Phytohormone profiling of Malus domestica and Chenopodium murale hairy root exudate - Association with Allelopathic Effects" in Journal of Plant Growth Regulation (2024),
https://doi.org/10.1007/s00344-024-11328-5 . .

TY  - CONF
AU  - Radulović, Natalija
AU  - Kostić, Olga
AU  - Košanin, Olivera
AU  - Pavlović, Dragana
AU  - Sekulić, Dimitrije
AU  - Jonjev, Milica
AU  - Mitrović, Miroslava
AU  - Pavlović, Pavle
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6823
AB  - Linden trees are one of the most popular tree species in European cities and also in Serbia, often used for planting on green areas (parks and tree lines) in Belgrade. In this study, individuals of silver linden Tilia tomentosa Moench. in the tree line (Bulevar Nikola Tesla) and city park (Park Ušće) in Belgrade were selected and the concentration of potentially toxic elements (PTEs) Cu, Pb and Zn in roots and leaves and in the soil (0-10cm and 10-30cm) under each individual as well as in the natural habitat on Fruška Gora mountain (control) were collected. To evaluate the potential of the selected species for phytoremediation (phytoextraction and phytostabilisation of PTEs), the bioaccumulation of roots and leaves (BCFroot and BCFleaf), and translocation factors (TF) were calculated. Site-dependent variations were observed for all analysed parameters. In the soil samples, the concentrations of Cu, Pb and Zn were above the average values for European soils only at urban sites (17.3 mg kg-1, 32 mg kg-1 and 68.1 mg kg-1, respectively) and in the critical range for plants for Zn (>70 mg kg-1) and for Cu (>60 mg kg-1) only in the tree line, while the concentration of Pb was above the MAC. A high correlation was found for the concentration of all tested elements in the soil and in the roots, and only for Zn in the leaves. Nevertheless, the levels of Cu and Pb in the roots and Cu in the leaves were within the normal range for plant tissues (5-30 mg kg-1, 5-10 mg kg-1) at all sites analysed, while Zn was in deficit (<20 mg kg-1). By analysing the bioconcentration and translocation factors for the measured elements, it was found that T. tomentosa has the potential for their phytostabilisation (BCFroot<1, BCFleaf<1, TF>1). These findings could be of great importance in the planning of sustainable management of urban areas.
PB  - Belgrade: Serbian Society of Corrosion and Materials Protection
C3  - Proceedings: 25th YuCorr International Conference: Meeting Point of the Science and Practice in the Fields of Corrosion, Materials and Environmental Protection; 2024 May 28-31; Divčibare, Serbia
T1  - Evaluation of the phytoremediation potential of Tilia tomentosa Moench. for Cu, Pb and Zn on green areas in Belgrade
SP  - 116
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6823
ER  - 

@conference{
author = "Radulović, Natalija and Kostić, Olga and Košanin, Olivera and Pavlović, Dragana and Sekulić, Dimitrije and Jonjev, Milica and Mitrović, Miroslava and Pavlović, Pavle",
year = "2024",
abstract = "Linden trees are one of the most popular tree species in European cities and also in Serbia, often used for planting on green areas (parks and tree lines) in Belgrade. In this study, individuals of silver linden Tilia tomentosa Moench. in the tree line (Bulevar Nikola Tesla) and city park (Park Ušće) in Belgrade were selected and the concentration of potentially toxic elements (PTEs) Cu, Pb and Zn in roots and leaves and in the soil (0-10cm and 10-30cm) under each individual as well as in the natural habitat on Fruška Gora mountain (control) were collected. To evaluate the potential of the selected species for phytoremediation (phytoextraction and phytostabilisation of PTEs), the bioaccumulation of roots and leaves (BCFroot and BCFleaf), and translocation factors (TF) were calculated. Site-dependent variations were observed for all analysed parameters. In the soil samples, the concentrations of Cu, Pb and Zn were above the average values for European soils only at urban sites (17.3 mg kg-1, 32 mg kg-1 and 68.1 mg kg-1, respectively) and in the critical range for plants for Zn (>70 mg kg-1) and for Cu (>60 mg kg-1) only in the tree line, while the concentration of Pb was above the MAC. A high correlation was found for the concentration of all tested elements in the soil and in the roots, and only for Zn in the leaves. Nevertheless, the levels of Cu and Pb in the roots and Cu in the leaves were within the normal range for plant tissues (5-30 mg kg-1, 5-10 mg kg-1) at all sites analysed, while Zn was in deficit (<20 mg kg-1). By analysing the bioconcentration and translocation factors for the measured elements, it was found that T. tomentosa has the potential for their phytostabilisation (BCFroot<1, BCFleaf<1, TF>1). These findings could be of great importance in the planning of sustainable management of urban areas.",
publisher = "Belgrade: Serbian Society of Corrosion and Materials Protection",
journal = "Proceedings: 25th YuCorr International Conference: Meeting Point of the Science and Practice in the Fields of Corrosion, Materials and Environmental Protection; 2024 May 28-31; Divčibare, Serbia",
title = "Evaluation of the phytoremediation potential of Tilia tomentosa Moench. for Cu, Pb and Zn on green areas in Belgrade",
pages = "116",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6823"
}

Radulović, N., Kostić, O., Košanin, O., Pavlović, D., Sekulić, D., Jonjev, M., Mitrović, M.,& Pavlović, P.. (2024). Evaluation of the phytoremediation potential of Tilia tomentosa Moench. for Cu, Pb and Zn on green areas in Belgrade. in Proceedings: 25th YuCorr International Conference: Meeting Point of the Science and Practice in the Fields of Corrosion, Materials and Environmental Protection; 2024 May 28-31; Divčibare, Serbia
Belgrade: Serbian Society of Corrosion and Materials Protection., 116.
https://hdl.handle.net/21.15107/rcub_ibiss_6823

Radulović N, Kostić O, Košanin O, Pavlović D, Sekulić D, Jonjev M, Mitrović M, Pavlović P. Evaluation of the phytoremediation potential of Tilia tomentosa Moench. for Cu, Pb and Zn on green areas in Belgrade. in Proceedings: 25th YuCorr International Conference: Meeting Point of the Science and Practice in the Fields of Corrosion, Materials and Environmental Protection; 2024 May 28-31; Divčibare, Serbia. 2024;:116.
https://hdl.handle.net/21.15107/rcub_ibiss_6823 .

Radulović, Natalija, Kostić, Olga, Košanin, Olivera, Pavlović, Dragana, Sekulić, Dimitrije, Jonjev, Milica, Mitrović, Miroslava, Pavlović, Pavle, "Evaluation of the phytoremediation potential of Tilia tomentosa Moench. for Cu, Pb and Zn on green areas in Belgrade" in Proceedings: 25th YuCorr International Conference: Meeting Point of the Science and Practice in the Fields of Corrosion, Materials and Environmental Protection; 2024 May 28-31; Divčibare, Serbia (2024):116,
https://hdl.handle.net/21.15107/rcub_ibiss_6823 .

TY  - CONF
AU  - Matić, Marija
AU  - Pavlović, Dragana
AU  - Perović, Veljko
AU  - Kostić, Olga
AU  - Jarić, Snežana
AU  - Mitrović, Miroslava
AU  - Pavlović, Pavle
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6824
AB  - Vegetable crops are one of the most important foods in the human diet and understanding their ability to accumulate toxic concentrations of hazardous elements can help reduce potential risks to human health. Solanum tuberosum L. is considered the most important non-cereal crop in the world and one of the most important foods in the diet, therefore it is particularly important to identify the potential health risks of consuming vegetables that may be grown in the immediate vicinity of coal mines and thermal power plants. Samples of S. tuberosum and associated soils were taken in the area of the municipalities of Lazarevac (village of Sokolovo) and Obrenovac (village of Krtinka) and Surčin (village of Jakovo) to determine the As and Pb content and to assess the potential human health risk associated with dietary exposure from consumption of the investigated crop. The results obtained showed that the content of non-essential and toxic elements As and Pb was within the range considered normal in plant tissues and below the MAC for dried vegetables under national legislation of the Republic of Serbia. Arsenic was not measured in the tubers of S. tuberosum in 2013, but was measured in the 2014 harvest, while the Pb concentration showed a decreasing trend between the two sampling seasons. The calculation of the non-carcinogenic risk showed that the target hazard quotient and the hazard risk index for S. tuberosum from 2014 exceeded the provisional maximum tolerable daily intake, as the values were >1. These results show that consumption of the 2014 harvest from the investigated sampling sites posed a health risk to humans and urge caution when growing S. tuberosum at the sampling sites.
PB  - Belgrade: Serbian Society of Corrosion and Materials Protection
C3  - Proceedings: 25th YuCorr International Conference: Meeting Point of the Science and Practice in the Fields of Corrosion, Materials and Environmental Protection; 2024 May 28-31; Divčibare, Serbia
T1  - Assessment of As and Pb in Solanum tuberosum L. from urban areas of Belgrade and potential dietary health risk for the population
SP  - 117
EP  - 123
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6824
ER  - 

@conference{
author = "Matić, Marija and Pavlović, Dragana and Perović, Veljko and Kostić, Olga and Jarić, Snežana and Mitrović, Miroslava and Pavlović, Pavle",
year = "2024",
abstract = "Vegetable crops are one of the most important foods in the human diet and understanding their ability to accumulate toxic concentrations of hazardous elements can help reduce potential risks to human health. Solanum tuberosum L. is considered the most important non-cereal crop in the world and one of the most important foods in the diet, therefore it is particularly important to identify the potential health risks of consuming vegetables that may be grown in the immediate vicinity of coal mines and thermal power plants. Samples of S. tuberosum and associated soils were taken in the area of the municipalities of Lazarevac (village of Sokolovo) and Obrenovac (village of Krtinka) and Surčin (village of Jakovo) to determine the As and Pb content and to assess the potential human health risk associated with dietary exposure from consumption of the investigated crop. The results obtained showed that the content of non-essential and toxic elements As and Pb was within the range considered normal in plant tissues and below the MAC for dried vegetables under national legislation of the Republic of Serbia. Arsenic was not measured in the tubers of S. tuberosum in 2013, but was measured in the 2014 harvest, while the Pb concentration showed a decreasing trend between the two sampling seasons. The calculation of the non-carcinogenic risk showed that the target hazard quotient and the hazard risk index for S. tuberosum from 2014 exceeded the provisional maximum tolerable daily intake, as the values were >1. These results show that consumption of the 2014 harvest from the investigated sampling sites posed a health risk to humans and urge caution when growing S. tuberosum at the sampling sites.",
publisher = "Belgrade: Serbian Society of Corrosion and Materials Protection",
journal = "Proceedings: 25th YuCorr International Conference: Meeting Point of the Science and Practice in the Fields of Corrosion, Materials and Environmental Protection; 2024 May 28-31; Divčibare, Serbia",
title = "Assessment of As and Pb in Solanum tuberosum L. from urban areas of Belgrade and potential dietary health risk for the population",
pages = "117-123",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6824"
}

Matić, M., Pavlović, D., Perović, V., Kostić, O., Jarić, S., Mitrović, M.,& Pavlović, P.. (2024). Assessment of As and Pb in Solanum tuberosum L. from urban areas of Belgrade and potential dietary health risk for the population. in Proceedings: 25th YuCorr International Conference: Meeting Point of the Science and Practice in the Fields of Corrosion, Materials and Environmental Protection; 2024 May 28-31; Divčibare, Serbia
Belgrade: Serbian Society of Corrosion and Materials Protection., 117-123.
https://hdl.handle.net/21.15107/rcub_ibiss_6824

Matić M, Pavlović D, Perović V, Kostić O, Jarić S, Mitrović M, Pavlović P. Assessment of As and Pb in Solanum tuberosum L. from urban areas of Belgrade and potential dietary health risk for the population. in Proceedings: 25th YuCorr International Conference: Meeting Point of the Science and Practice in the Fields of Corrosion, Materials and Environmental Protection; 2024 May 28-31; Divčibare, Serbia. 2024;:117-123.
https://hdl.handle.net/21.15107/rcub_ibiss_6824 .

Matić, Marija, Pavlović, Dragana, Perović, Veljko, Kostić, Olga, Jarić, Snežana, Mitrović, Miroslava, Pavlović, Pavle, "Assessment of As and Pb in Solanum tuberosum L. from urban areas of Belgrade and potential dietary health risk for the population" in Proceedings: 25th YuCorr International Conference: Meeting Point of the Science and Practice in the Fields of Corrosion, Materials and Environmental Protection; 2024 May 28-31; Divčibare, Serbia (2024):117-123,
https://hdl.handle.net/21.15107/rcub_ibiss_6824 .

TY  - CONF
AU  - Pavlović, Dragana
AU  - Matić, Marija
AU  - Perović, Veljko
AU  - Jarić, Snežana
AU  - Kostić, Olga
AU  - Mitrović, Miroslava
AU  - Pavlović, Pavle
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6825
AB  - The health consequences of eating contaminated vegetables for the population are a major problem worldwide. In this study, As and Pb concentrations in soil and in Allium cepa L. (spring onions and onions) from three Belgrade municipalities were determined using an inductively coupled plasma optical emission spectrophotometer (ICP-OES) and possible health risks from onion consumption were assessed. The results showed that As and Pb concentrations in the soil were within the maximum levels proposed by national legislation; the highest concentrations were measured in the soil of Lazarevac. It was found that although A. cepa has a low potential to accumulate As and Pb, this vegetable accumulates As and Pb at levels well above the limits set by the World Health Organisation, so great caution should be exercised when consuming it. The consumption of spring onions could lead to serious health problems over time, especially with regard to the increased As content, although the onions have been shown to be safe for consumption. This study could contribute to a better understanding of the health risks associated with the consumption of vegetables grown near urban and industrial areas.
PB  - Belgrade: Serbian Society of Corrosion and Materials Protection
C3  - Proceedings: 25th YuCorr International Conference: Meeting Point of the Science and Practice in the Fields of Corrosion, Materials and Environmental Protection; 2024 May 28-31; Divčibare, Serbia
T1  - Levels of As and Pb in soil and Allium cepa L. and associated health risk in three Belgrade municipalities
SP  - 39
EP  - 44
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6825
ER  - 

@conference{
author = "Pavlović, Dragana and Matić, Marija and Perović, Veljko and Jarić, Snežana and Kostić, Olga and Mitrović, Miroslava and Pavlović, Pavle",
year = "2024",
abstract = "The health consequences of eating contaminated vegetables for the population are a major problem worldwide. In this study, As and Pb concentrations in soil and in Allium cepa L. (spring onions and onions) from three Belgrade municipalities were determined using an inductively coupled plasma optical emission spectrophotometer (ICP-OES) and possible health risks from onion consumption were assessed. The results showed that As and Pb concentrations in the soil were within the maximum levels proposed by national legislation; the highest concentrations were measured in the soil of Lazarevac. It was found that although A. cepa has a low potential to accumulate As and Pb, this vegetable accumulates As and Pb at levels well above the limits set by the World Health Organisation, so great caution should be exercised when consuming it. The consumption of spring onions could lead to serious health problems over time, especially with regard to the increased As content, although the onions have been shown to be safe for consumption. This study could contribute to a better understanding of the health risks associated with the consumption of vegetables grown near urban and industrial areas.",
publisher = "Belgrade: Serbian Society of Corrosion and Materials Protection",
journal = "Proceedings: 25th YuCorr International Conference: Meeting Point of the Science and Practice in the Fields of Corrosion, Materials and Environmental Protection; 2024 May 28-31; Divčibare, Serbia",
title = "Levels of As and Pb in soil and Allium cepa L. and associated health risk in three Belgrade municipalities",
pages = "39-44",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6825"
}

Pavlović, D., Matić, M., Perović, V., Jarić, S., Kostić, O., Mitrović, M.,& Pavlović, P.. (2024). Levels of As and Pb in soil and Allium cepa L. and associated health risk in three Belgrade municipalities. in Proceedings: 25th YuCorr International Conference: Meeting Point of the Science and Practice in the Fields of Corrosion, Materials and Environmental Protection; 2024 May 28-31; Divčibare, Serbia
Belgrade: Serbian Society of Corrosion and Materials Protection., 39-44.
https://hdl.handle.net/21.15107/rcub_ibiss_6825

Pavlović D, Matić M, Perović V, Jarić S, Kostić O, Mitrović M, Pavlović P. Levels of As and Pb in soil and Allium cepa L. and associated health risk in three Belgrade municipalities. in Proceedings: 25th YuCorr International Conference: Meeting Point of the Science and Practice in the Fields of Corrosion, Materials and Environmental Protection; 2024 May 28-31; Divčibare, Serbia. 2024;:39-44.
https://hdl.handle.net/21.15107/rcub_ibiss_6825 .

Pavlović, Dragana, Matić, Marija, Perović, Veljko, Jarić, Snežana, Kostić, Olga, Mitrović, Miroslava, Pavlović, Pavle, "Levels of As and Pb in soil and Allium cepa L. and associated health risk in three Belgrade municipalities" in Proceedings: 25th YuCorr International Conference: Meeting Point of the Science and Practice in the Fields of Corrosion, Materials and Environmental Protection; 2024 May 28-31; Divčibare, Serbia (2024):39-44,
https://hdl.handle.net/21.15107/rcub_ibiss_6825 .

TY  - JOUR
AU  - Ninković, Slavica
AU  - Motyka, Václav
AU  - Stanišić, Mariana
AU  - Smailagić, Dijana
AU  - Živanović, Branka
AU  - Dobrev, Petre I.
AU  - Banjac, Nevena
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6826
AB  - Compounds exuded from roots play a key role in regulating plant allelopathic interactions. However, phytochormone profiling of root exudates and their contribution to an overall allelochemical activity of specific plant species is neglected topic in allelochemical research.  Hairy root growth media of two different species, the fruit tree species  Malus × domestica Borkh. and the herbaceous weed species Chenopodium murale L. were collected and analyzed by high-performance liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). We found that most of the phytohormones exuded by the hairy roots of C. murale and M. domestica were associated with the acidic fraction (96.8% and 98.9%, respectively), including 2-oxindole-3-acetic acid, phenylacetic acid, salicylic acid (SA), benzoic acid (BzA), and abscisic acid, with SA and BzA being the most abundant, while those associated with the basic fraction, including cytokinins and the ethylene precursor 1-aminocyclopropane-1-carboxylic acid, accounted for only 1% of the plant growth substances detected in both species. Exogenous application of 0.2 µM SA, which was released from the hairy roots of C. murale and accumulated in the culture media for four weeks, significantly impaired hairy root growth of M. domestica and also shoot and root growth of Arabidopsis seedlings. The disruptive effect of 0.2 µM SA on the membrane potential of M. domestica hairy root and Arabidopsis root cells was determined. The data obtained could be useful for planning further studies aimed at clarifying the contribution and role of exuded phytohormones to the overall allelopathic potential of these two plant species.
PB  - New York: Springer
T2  - Journal of Plant Growth Regulation
T1  - Phytohormone profiling of Malus domestica and Chenopodium murale hairy root exudate - Association with Allelopathic Effects
DO  - 10.1007/s00344-024-11328-5
ER  - 

@article{
author = "Ninković, Slavica and Motyka, Václav and Stanišić, Mariana and Smailagić, Dijana and Živanović, Branka and Dobrev, Petre I. and Banjac, Nevena",
year = "2024",
abstract = "Compounds exuded from roots play a key role in regulating plant allelopathic interactions. However, phytochormone profiling of root exudates and their contribution to an overall allelochemical activity of specific plant species is neglected topic in allelochemical research.  Hairy root growth media of two different species, the fruit tree species  Malus × domestica Borkh. and the herbaceous weed species Chenopodium murale L. were collected and analyzed by high-performance liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). We found that most of the phytohormones exuded by the hairy roots of C. murale and M. domestica were associated with the acidic fraction (96.8% and 98.9%, respectively), including 2-oxindole-3-acetic acid, phenylacetic acid, salicylic acid (SA), benzoic acid (BzA), and abscisic acid, with SA and BzA being the most abundant, while those associated with the basic fraction, including cytokinins and the ethylene precursor 1-aminocyclopropane-1-carboxylic acid, accounted for only 1% of the plant growth substances detected in both species. Exogenous application of 0.2 µM SA, which was released from the hairy roots of C. murale and accumulated in the culture media for four weeks, significantly impaired hairy root growth of M. domestica and also shoot and root growth of Arabidopsis seedlings. The disruptive effect of 0.2 µM SA on the membrane potential of M. domestica hairy root and Arabidopsis root cells was determined. The data obtained could be useful for planning further studies aimed at clarifying the contribution and role of exuded phytohormones to the overall allelopathic potential of these two plant species.",
publisher = "New York: Springer",
journal = "Journal of Plant Growth Regulation",
title = "Phytohormone profiling of Malus domestica and Chenopodium murale hairy root exudate - Association with Allelopathic Effects",
doi = "10.1007/s00344-024-11328-5"
}

Ninković, S., Motyka, V., Stanišić, M., Smailagić, D., Živanović, B., Dobrev, P. I.,& Banjac, N.. (2024). Phytohormone profiling of Malus domestica and Chenopodium murale hairy root exudate - Association with Allelopathic Effects. in Journal of Plant Growth Regulation
New York: Springer..
https://doi.org/10.1007/s00344-024-11328-5

Ninković S, Motyka V, Stanišić M, Smailagić D, Živanović B, Dobrev PI, Banjac N. Phytohormone profiling of Malus domestica and Chenopodium murale hairy root exudate - Association with Allelopathic Effects. in Journal of Plant Growth Regulation. 2024;.
doi:10.1007/s00344-024-11328-5 .

Ninković, Slavica, Motyka, Václav, Stanišić, Mariana, Smailagić, Dijana, Živanović, Branka, Dobrev, Petre I., Banjac, Nevena, "Phytohormone profiling of Malus domestica and Chenopodium murale hairy root exudate - Association with Allelopathic Effects" in Journal of Plant Growth Regulation (2024),
https://doi.org/10.1007/s00344-024-11328-5 . .

TY  - JOUR
AU  - Baur, Julian
AU  - Koppik, Mareike
AU  - Savković, Uroš
AU  - Đorđević, Mirko
AU  - Stojković, Biljana
AU  - Berger, David
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6820
AB  - An often-overlooked aspect of life-history optimization is the allocation of resources to protect the germline and secure safe transmission of genetic information. While failure to do so renders significant fitness consequences in future generations, germline maintenance comes with substantial costs. Thus, germline allocation should trade off with other life-history decisions and be optimized in accordance with an organism’s reproductive schedule. Here, we tested this hypothesis by studying germline maintenance in lines of seed beetle, selected for early (E) or late (L) reproduction for 350 and 240 generations, respectively. Female animals provide maintenance and screening of male gametes in their reproductive tract and oocytes. Here, we reveal the ability of young and aged E- and L-females to provide this form of germline maintenance by mating them to males with ejaculates with artificially elevated levels of protein and DNA damage. We find that germline maintenance in E-females peaks at young age and then declines, while the opposite is true for L-females, in accordance with the age of reproduction in the respective regime. These findings identify the central role of allocation to secure germline integrity in life-history evolution and highlight how females can play a crucial role in mitigating the effects of male germline decisions on mutation rate and offspring quality.
PB  - The Royal Society
T2  - Proceedings of the Royal Society. B: Biological Sciences
T1  - Coevolution of longevity and femalegermline maintenance
IS  - 2024
VL  - 291
DO  - 10.1098/rspb.2024.0532
SP  - 20240532
ER  - 

@article{
author = "Baur, Julian and Koppik, Mareike and Savković, Uroš and Đorđević, Mirko and Stojković, Biljana and Berger, David",
year = "2024",
abstract = "An often-overlooked aspect of life-history optimization is the allocation of resources to protect the germline and secure safe transmission of genetic information. While failure to do so renders significant fitness consequences in future generations, germline maintenance comes with substantial costs. Thus, germline allocation should trade off with other life-history decisions and be optimized in accordance with an organism’s reproductive schedule. Here, we tested this hypothesis by studying germline maintenance in lines of seed beetle, selected for early (E) or late (L) reproduction for 350 and 240 generations, respectively. Female animals provide maintenance and screening of male gametes in their reproductive tract and oocytes. Here, we reveal the ability of young and aged E- and L-females to provide this form of germline maintenance by mating them to males with ejaculates with artificially elevated levels of protein and DNA damage. We find that germline maintenance in E-females peaks at young age and then declines, while the opposite is true for L-females, in accordance with the age of reproduction in the respective regime. These findings identify the central role of allocation to secure germline integrity in life-history evolution and highlight how females can play a crucial role in mitigating the effects of male germline decisions on mutation rate and offspring quality.",
publisher = "The Royal Society",
journal = "Proceedings of the Royal Society. B: Biological Sciences",
title = "Coevolution of longevity and femalegermline maintenance",
number = "2024",
volume = "291",
doi = "10.1098/rspb.2024.0532",
pages = "20240532"
}

Baur, J., Koppik, M., Savković, U., Đorđević, M., Stojković, B.,& Berger, D.. (2024). Coevolution of longevity and femalegermline maintenance. in Proceedings of the Royal Society. B: Biological Sciences
The Royal Society., 291(2024), 20240532.
https://doi.org/10.1098/rspb.2024.0532

Baur J, Koppik M, Savković U, Đorđević M, Stojković B, Berger D. Coevolution of longevity and femalegermline maintenance. in Proceedings of the Royal Society. B: Biological Sciences. 2024;291(2024):20240532.
doi:10.1098/rspb.2024.0532 .

Baur, Julian, Koppik, Mareike, Savković, Uroš, Đorđević, Mirko, Stojković, Biljana, Berger, David, "Coevolution of longevity and femalegermline maintenance" in Proceedings of the Royal Society. B: Biological Sciences, 291, no. 2024 (2024):20240532,
https://doi.org/10.1098/rspb.2024.0532 . .

TY  - JOUR
AU  - Kasalović, Marijana P.
AU  - Jelača, Sanja
AU  - Milanović, Žiko
AU  - Maksimović-Ivanić, Danijela
AU  - Mijatović, Sanja
AU  - Lađarević, Jelena
AU  - Božić, Bojan
AU  - Marković, Zoran
AU  - Dunđerović, Duško
AU  - Rüffer, Tobias
AU  - Kretschmer, Robert
AU  - Kaluđerović, Goran N.
AU  - Pantelić, Nebojša Đ.
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6821
AB  - Three newly synthesized triphenyltin(IV) compounds, Ph3SnL1 (L1− = 3-(4-methyl-2-oxoquinolin-1(2H)-yl)propanoato), Ph3SnL2 (L2− = 2-(4-methyl-2-oxoquinolin-1(2H)-yl)ethanoato), and Ph3SnL3 (L3− = 2-(4-hydroxy-2-oxoquinolin-1(2H)-yl)ethanoato), were characterized by elemental microanalysis, FT-IR spectroscopy and multinuclear (1H, 13C and 119Sn) NMR spectroscopy. A single X-ray diffraction study indicates that compounds Ph3SnL1 and Ph3SnL2 exhibit a 1D zig-zag chain polymeric structure, which in the case of Ph3SnL2 is additionally stabilized by π-interactions. In addition, the synthesized compounds were further examined using density functional theory and natural bond orbital analysis. The compounds have been evaluated for their in vitro anticancer activity against three human cell lines: MCF-7 (breast adenocarcinoma), A375 (melanoma), HCT116 (colorectal carcinoma), and three murine cell lines: 4T1 (breast carcinoma), B16 (melanoma), CT26 (colon carcinoma) using MTT and CV assays. The IC50 values fall in the nanomolar range, indicating that these compounds possess better anticancer activity than cisplatin. The study of the effect of the newly developed drug Ph3SnL1 showed its plasticity in achieving an antitumor effect in vitro, which depends on the specificity of the phenotype and the redox status of the malignant cell line and ranges from the initiation of apoptotic cell death to the induction of differentiation to a more mature cell form. In the syngeneic model of murine melanoma, Ph3SnL1 showed the potential to reduce the tumor volume similar to cisplatin, but in a well-tolerated form and with low systemic toxicity, representing a significant advantage over the conventional drug.
PB  - Cambridge: The Royal Society of Chemistry
T2  - Dalton Transactions
T1  - Novel triphenyltin(iv) compounds with carboxylato N-functionalized 2-quinolones as promising potential anticancer drug candidates: in vitro and in vivo evaluation
IS  - 19
VL  - 53
DO  - 10.1039/D4DT00182F
SP  - 8298
EP  - 8314
ER  - 

@article{
author = "Kasalović, Marijana P. and Jelača, Sanja and Milanović, Žiko and Maksimović-Ivanić, Danijela and Mijatović, Sanja and Lađarević, Jelena and Božić, Bojan and Marković, Zoran and Dunđerović, Duško and Rüffer, Tobias and Kretschmer, Robert and Kaluđerović, Goran N. and Pantelić, Nebojša Đ.",
year = "2024",
abstract = "Three newly synthesized triphenyltin(IV) compounds, Ph3SnL1 (L1− = 3-(4-methyl-2-oxoquinolin-1(2H)-yl)propanoato), Ph3SnL2 (L2− = 2-(4-methyl-2-oxoquinolin-1(2H)-yl)ethanoato), and Ph3SnL3 (L3− = 2-(4-hydroxy-2-oxoquinolin-1(2H)-yl)ethanoato), were characterized by elemental microanalysis, FT-IR spectroscopy and multinuclear (1H, 13C and 119Sn) NMR spectroscopy. A single X-ray diffraction study indicates that compounds Ph3SnL1 and Ph3SnL2 exhibit a 1D zig-zag chain polymeric structure, which in the case of Ph3SnL2 is additionally stabilized by π-interactions. In addition, the synthesized compounds were further examined using density functional theory and natural bond orbital analysis. The compounds have been evaluated for their in vitro anticancer activity against three human cell lines: MCF-7 (breast adenocarcinoma), A375 (melanoma), HCT116 (colorectal carcinoma), and three murine cell lines: 4T1 (breast carcinoma), B16 (melanoma), CT26 (colon carcinoma) using MTT and CV assays. The IC50 values fall in the nanomolar range, indicating that these compounds possess better anticancer activity than cisplatin. The study of the effect of the newly developed drug Ph3SnL1 showed its plasticity in achieving an antitumor effect in vitro, which depends on the specificity of the phenotype and the redox status of the malignant cell line and ranges from the initiation of apoptotic cell death to the induction of differentiation to a more mature cell form. In the syngeneic model of murine melanoma, Ph3SnL1 showed the potential to reduce the tumor volume similar to cisplatin, but in a well-tolerated form and with low systemic toxicity, representing a significant advantage over the conventional drug.",
publisher = "Cambridge: The Royal Society of Chemistry",
journal = "Dalton Transactions",
title = "Novel triphenyltin(iv) compounds with carboxylato N-functionalized 2-quinolones as promising potential anticancer drug candidates: in vitro and in vivo evaluation",
number = "19",
volume = "53",
doi = "10.1039/D4DT00182F",
pages = "8298-8314"
}

Kasalović, M. P., Jelača, S., Milanović, Ž., Maksimović-Ivanić, D., Mijatović, S., Lađarević, J., Božić, B., Marković, Z., Dunđerović, D., Rüffer, T., Kretschmer, R., Kaluđerović, G. N.,& Pantelić, N. Đ.. (2024). Novel triphenyltin(iv) compounds with carboxylato N-functionalized 2-quinolones as promising potential anticancer drug candidates: in vitro and in vivo evaluation. in Dalton Transactions
Cambridge: The Royal Society of Chemistry., 53(19), 8298-8314.
https://doi.org/10.1039/D4DT00182F

Kasalović MP, Jelača S, Milanović Ž, Maksimović-Ivanić D, Mijatović S, Lađarević J, Božić B, Marković Z, Dunđerović D, Rüffer T, Kretschmer R, Kaluđerović GN, Pantelić NĐ. Novel triphenyltin(iv) compounds with carboxylato N-functionalized 2-quinolones as promising potential anticancer drug candidates: in vitro and in vivo evaluation. in Dalton Transactions. 2024;53(19):8298-8314.
doi:10.1039/D4DT00182F .

Kasalović, Marijana P., Jelača, Sanja, Milanović, Žiko, Maksimović-Ivanić, Danijela, Mijatović, Sanja, Lađarević, Jelena, Božić, Bojan, Marković, Zoran, Dunđerović, Duško, Rüffer, Tobias, Kretschmer, Robert, Kaluđerović, Goran N., Pantelić, Nebojša Đ., "Novel triphenyltin(iv) compounds with carboxylato N-functionalized 2-quinolones as promising potential anticancer drug candidates: in vitro and in vivo evaluation" in Dalton Transactions, 53, no. 19 (2024):8298-8314,
https://doi.org/10.1039/D4DT00182F . .

TY  - THES
AU  - Tatalović, Nikola
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6819
AB  - Ibogain je indolni alkaloid izolovan iz korena iboge (Tabernanthe iboga Baill.) koji se u alternativnoj medicini koristi kao antiadiktivni agens čija je upotreba povezana sa rizicima koji uključuju aritmije sa smrtnim ishodom. Pored neposrednih farmakoloških efekata koji se ostvaruju interakcijom sa više tipova receptora, ibogain još uvek nepoznatim mehanizmom dovodi do pražnjenja ATP rezervi, povećanja stope energetskog metabolizma i stvaranja ROS, kao i do povećanja aktivnosti antioksidativnih enzima. Cilj ove disertacije je bio da kombinacijom in vivo (per os) tretmana i ex vivo eksperimenata na izolovanim organima utvrdi vrstu i mehanizme fiziološke reaktivnosti ibogaina, i eventualne ROS posredovane efekte. Pošto su biološka dostupnost ibogaina i efekti na nivou CNS veći kod ženki nego kod mužjaka, eksperimenti u ovoj disertaciji su izvedeni na ženkama. Pokazano je da per os tretman ima umeren glikogenolitički efekat u jetri. Promene u redoks ravnoteži su izrazito tkivno specifične bez jasne dozne (1 ili 20 mg/kg) ili vremenske (6 ili 24 sata) zavisnosti. Ibogain izaziva akutnu nekrozu kardiomiocita koja bi mogla da bude uzrok potencijalno fatalnih aritmija. Mehanizam delovanja ibogaina na fiziološkom nivou nije direktno povezan sa promenom koncentracije ATP, već uključuje dominantno interakciju sa receptorima: farmakološki efekti na kontraktilnu aktivnost izolovanog uterusa se ostvaruju interakcijom sa 5-HT receptorima, dok su promene u aktivnosti antioksidativnih enzima posredovane β-adrenergičkim receptorima i KATP kanalima. Njihova blokada sprečava porast aktivnosti antioksidativnih enzima. Iako smatran generalnim pro-antioksidantom, efekti ibogaina su izrazito tkivno specifični i zavise od zastupljenosti receptora preko kojih ibogain ostvaruje svoje efekat.
AB  - Ibogaine is an indole alkaloid isolated from the iboga root (Tabernanthe iboga Baill.) and is used in alternative medicine as an anti-addictive drug whose use is associated with risks such as fatal cardiac arrhythmia. In addition to the direct pharmacological effects achieved through interaction with different types of receptors, ibogaine leads to the depletion of ATP reserves, an increase in the rate of energy metabolism and the formation of ROS, and an increase in the activity of antioxidant enzymes via an as yet unknown mechanism. The aim of this dissertation was to determine the nature and mechanisms of the physiological reactivity of ibogaine and possible ROS-mediated effects by a combination of in vivo treatment (per os) and ex vivo experiments on isolated organs. Since the bioavailability of ibogaine and the effects at the CNS level are greater in females than in males, the experiments in this dissertation were performed in females. Per os treatment was shown to have a moderate glycogenolytic effect in the liver. The changes in redox balance are highly tissue specific, with no clear dose (1 or 20 mg/kg) or time (6 or 24 hours) dependence. Ibogaine causes acute necrosis of cardiomyocytes, which could be the cause of potentially fatal cardiac arrhythmias. The mechanism of action of ibogaine at the physiological level is not directly related to the change in ATP concentration, but mainly involves interaction with receptors: pharmacological effects on the contractile activity of the isolated uterus are achieved through interaction with 5-HT receptors, while changes in the activity of antioxidant enzymes are mediated by β-adrenergic receptors and KATP channels. Their blockade prevents the increase in antioxidant enzyme activity. Although it is considered a general pro-antioxidant, the effects of ibogaine are very tissue-specific and depend on the presence of the receptors through which ibogaine achieves its effects.
PB  - Belgrade: University of Belgrad, Faculty of Biology
T1  - Redoks-posredovani mehanizmi farmakoloških efekata ibogaina na ženke pacova
T1  - Redox-mediated mechanisms of ibogaine pharmacological effects on female rats
SP  - 1
EP  - 130
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6819
ER  - 

@phdthesis{
author = "Tatalović, Nikola",
year = "2024",
abstract = "Ibogain je indolni alkaloid izolovan iz korena iboge (Tabernanthe iboga Baill.) koji se u alternativnoj medicini koristi kao antiadiktivni agens čija je upotreba povezana sa rizicima koji uključuju aritmije sa smrtnim ishodom. Pored neposrednih farmakoloških efekata koji se ostvaruju interakcijom sa više tipova receptora, ibogain još uvek nepoznatim mehanizmom dovodi do pražnjenja ATP rezervi, povećanja stope energetskog metabolizma i stvaranja ROS, kao i do povećanja aktivnosti antioksidativnih enzima. Cilj ove disertacije je bio da kombinacijom in vivo (per os) tretmana i ex vivo eksperimenata na izolovanim organima utvrdi vrstu i mehanizme fiziološke reaktivnosti ibogaina, i eventualne ROS posredovane efekte. Pošto su biološka dostupnost ibogaina i efekti na nivou CNS veći kod ženki nego kod mužjaka, eksperimenti u ovoj disertaciji su izvedeni na ženkama. Pokazano je da per os tretman ima umeren glikogenolitički efekat u jetri. Promene u redoks ravnoteži su izrazito tkivno specifične bez jasne dozne (1 ili 20 mg/kg) ili vremenske (6 ili 24 sata) zavisnosti. Ibogain izaziva akutnu nekrozu kardiomiocita koja bi mogla da bude uzrok potencijalno fatalnih aritmija. Mehanizam delovanja ibogaina na fiziološkom nivou nije direktno povezan sa promenom koncentracije ATP, već uključuje dominantno interakciju sa receptorima: farmakološki efekti na kontraktilnu aktivnost izolovanog uterusa se ostvaruju interakcijom sa 5-HT receptorima, dok su promene u aktivnosti antioksidativnih enzima posredovane β-adrenergičkim receptorima i KATP kanalima. Njihova blokada sprečava porast aktivnosti antioksidativnih enzima. Iako smatran generalnim pro-antioksidantom, efekti ibogaina su izrazito tkivno specifični i zavise od zastupljenosti receptora preko kojih ibogain ostvaruje svoje efekat., Ibogaine is an indole alkaloid isolated from the iboga root (Tabernanthe iboga Baill.) and is used in alternative medicine as an anti-addictive drug whose use is associated with risks such as fatal cardiac arrhythmia. In addition to the direct pharmacological effects achieved through interaction with different types of receptors, ibogaine leads to the depletion of ATP reserves, an increase in the rate of energy metabolism and the formation of ROS, and an increase in the activity of antioxidant enzymes via an as yet unknown mechanism. The aim of this dissertation was to determine the nature and mechanisms of the physiological reactivity of ibogaine and possible ROS-mediated effects by a combination of in vivo treatment (per os) and ex vivo experiments on isolated organs. Since the bioavailability of ibogaine and the effects at the CNS level are greater in females than in males, the experiments in this dissertation were performed in females. Per os treatment was shown to have a moderate glycogenolytic effect in the liver. The changes in redox balance are highly tissue specific, with no clear dose (1 or 20 mg/kg) or time (6 or 24 hours) dependence. Ibogaine causes acute necrosis of cardiomyocytes, which could be the cause of potentially fatal cardiac arrhythmias. The mechanism of action of ibogaine at the physiological level is not directly related to the change in ATP concentration, but mainly involves interaction with receptors: pharmacological effects on the contractile activity of the isolated uterus are achieved through interaction with 5-HT receptors, while changes in the activity of antioxidant enzymes are mediated by β-adrenergic receptors and KATP channels. Their blockade prevents the increase in antioxidant enzyme activity. Although it is considered a general pro-antioxidant, the effects of ibogaine are very tissue-specific and depend on the presence of the receptors through which ibogaine achieves its effects.",
publisher = "Belgrade: University of Belgrad, Faculty of Biology",
title = "Redoks-posredovani mehanizmi farmakoloških efekata ibogaina na ženke pacova, Redox-mediated mechanisms of ibogaine pharmacological effects on female rats",
pages = "1-130",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6819"
}

Tatalović, N.. (2024). Redoks-posredovani mehanizmi farmakoloških efekata ibogaina na ženke pacova. 
Belgrade: University of Belgrad, Faculty of Biology., 1-130.
https://hdl.handle.net/21.15107/rcub_ibiss_6819

Tatalović N. Redoks-posredovani mehanizmi farmakoloških efekata ibogaina na ženke pacova. 2024;:1-130.
https://hdl.handle.net/21.15107/rcub_ibiss_6819 .

Tatalović, Nikola, "Redoks-posredovani mehanizmi farmakoloških efekata ibogaina na ženke pacova" (2024):1-130,
https://hdl.handle.net/21.15107/rcub_ibiss_6819 .

TY  - JOUR
AU  - Stanisavljević, Suzana
AU  - Stegnjaić, Goran
AU  - Jevtić, Bojan
AU  - Dimitrijević, Mirjana
AU  - Miljković, Đorđe
AU  - Lavrnja, Irena
AU  - Nikolovski, Neda
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6808
AB  - Ethyl pyruvate (EP) is a redox-active compound that has been previously shown to be
effective in restraining immune hyperactivity in animal models of various autoimmune and chronic
inflammatory diseases. Importantly, EP has also been proven to have a potent tolerogenic effect
on dendritic cells (DCs). Here, the influence of EP on the signaling pathways in DCs relevant for
their tolerogenicity, including anti-inflammatory NRF2 and pro-inflammatory NF-κB, was explored.
Specifically, the effects of EP on DCs obtained by GM-CSF-directed differentiation of murine bone
marrow precursor cells and matured under the influence of lipopolysaccharide (LPS) were examined via immunocytochemistry and RT-PCR. EP counteracted LPS-imposed morphological changes
and down-regulated the LPS-induced expression of pro-inflammatory mediators in DCs. While it
reduced the activation of NF-κB, EP potentiated NRF2 and downstream antioxidative molecules,
thus implying the regulation of NRF2 signaling pathways as the major reason for the tolerizing
effects of EP on DCs.
PB  - Basel: MDPI
T2  - International Journal of Molecular Sciences
T1  - NRF2 plays a crucial role in the tolerogenic effect of ethyl pyruvate on dendritic cells
IS  - 11
VL  - 25
DO  - 10.3390/ijms25116195
SP  - 6195
ER  - 

@article{
author = "Stanisavljević, Suzana and Stegnjaić, Goran and Jevtić, Bojan and Dimitrijević, Mirjana and Miljković, Đorđe and Lavrnja, Irena and Nikolovski, Neda",
year = "2024",
abstract = "Ethyl pyruvate (EP) is a redox-active compound that has been previously shown to be
effective in restraining immune hyperactivity in animal models of various autoimmune and chronic
inflammatory diseases. Importantly, EP has also been proven to have a potent tolerogenic effect
on dendritic cells (DCs). Here, the influence of EP on the signaling pathways in DCs relevant for
their tolerogenicity, including anti-inflammatory NRF2 and pro-inflammatory NF-κB, was explored.
Specifically, the effects of EP on DCs obtained by GM-CSF-directed differentiation of murine bone
marrow precursor cells and matured under the influence of lipopolysaccharide (LPS) were examined via immunocytochemistry and RT-PCR. EP counteracted LPS-imposed morphological changes
and down-regulated the LPS-induced expression of pro-inflammatory mediators in DCs. While it
reduced the activation of NF-κB, EP potentiated NRF2 and downstream antioxidative molecules,
thus implying the regulation of NRF2 signaling pathways as the major reason for the tolerizing
effects of EP on DCs.",
publisher = "Basel: MDPI",
journal = "International Journal of Molecular Sciences",
title = "NRF2 plays a crucial role in the tolerogenic effect of ethyl pyruvate on dendritic cells",
number = "11",
volume = "25",
doi = "10.3390/ijms25116195",
pages = "6195"
}

Stanisavljević, S., Stegnjaić, G., Jevtić, B., Dimitrijević, M., Miljković, Đ., Lavrnja, I.,& Nikolovski, N.. (2024). NRF2 plays a crucial role in the tolerogenic effect of ethyl pyruvate on dendritic cells. in International Journal of Molecular Sciences
Basel: MDPI., 25(11), 6195.
https://doi.org/10.3390/ijms25116195

Stanisavljević S, Stegnjaić G, Jevtić B, Dimitrijević M, Miljković Đ, Lavrnja I, Nikolovski N. NRF2 plays a crucial role in the tolerogenic effect of ethyl pyruvate on dendritic cells. in International Journal of Molecular Sciences. 2024;25(11):6195.
doi:10.3390/ijms25116195 .

Stanisavljević, Suzana, Stegnjaić, Goran, Jevtić, Bojan, Dimitrijević, Mirjana, Miljković, Đorđe, Lavrnja, Irena, Nikolovski, Neda, "NRF2 plays a crucial role in the tolerogenic effect of ethyl pyruvate on dendritic cells" in International Journal of Molecular Sciences, 25, no. 11 (2024):6195,
https://doi.org/10.3390/ijms25116195 . .

TY  - JOUR
AU  - Popržen, Tatjana
AU  - Jevremović, Slađana
AU  - Milošević, Snežana
AU  - Đurić, Marija
AU  - Uzelac, Branka
AU  - Stanković, Slaviša
AU  - Radulović, Olga
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6796
AB  - Aquatic plants, just like terrestrial plants, are exposed to oxidative stress. However,
their responses are still under-researched. In this study, we examined the physiological and antioxidative
responses of an aquatic plant, duckweed (Lemna minor L.), to four indole-3-acetic acid
(IAA)-degrading and -producing Pseudomonas bacteria (Pseudomonas oryzihabitans D1-104/3, P. putida
A3-104/5, P. gessardii C31-106/3 and P. yamanorum C44-104/1) and/or a supraphysiological level
of IAA (10 mg L−1). Growth characteristics, total photosynthetic pigment content, histochemical
localization of reactive oxygen species and antioxidant enzyme activity (SOD, CAT and POX) were
evaluated at two time points, after 3 and 7 days of co-cultivation. Superoxide anion and hydrogen
peroxide were produced and accumulated mainly in the roots, daughter fronds and veins of duckweeds.
Duckweeds’ responses depended on the strain of Pseudomonas, time and exogenous IAA.
Co-cultivation of duckweed with bacteria has positive or neutral effects. Exogenous application
of IAA had a negative or neutral effect on enzyme activity and other parameters. Co-cultivation
with P. gessardii C31-106/3 showed plant-growth-promoting effects on duckweed: increased biomass
production, modulation of duckweeds’ antioxidant enzymatic activity and reduction in hydrogen
peroxide content. This study widens our knowledge of aquatic plants and their response to oxidative
stress, supports the hypothesis that plant growth-promoting bacteria (PGPB) induce tolerable levels
of oxidative stress in plants and introduces a new PGPB strain, P. gessardii C31-106/3.
PB  - Basel: MDPI
T2  - Horticulturae
T1  - Antioxidative Response of Duckweed (Lemna minor L.) to Rhizosphere-Associated Pseudomonas Strains and Exogenous Indole-3-Acetic Acid
IS  - 6
VL  - 10
DO  - 10.3390/horticulturae10060562
SP  - 562
ER  - 

@article{
author = "Popržen, Tatjana and Jevremović, Slađana and Milošević, Snežana and Đurić, Marija and Uzelac, Branka and Stanković, Slaviša and Radulović, Olga",
year = "2024",
abstract = "Aquatic plants, just like terrestrial plants, are exposed to oxidative stress. However,
their responses are still under-researched. In this study, we examined the physiological and antioxidative
responses of an aquatic plant, duckweed (Lemna minor L.), to four indole-3-acetic acid
(IAA)-degrading and -producing Pseudomonas bacteria (Pseudomonas oryzihabitans D1-104/3, P. putida
A3-104/5, P. gessardii C31-106/3 and P. yamanorum C44-104/1) and/or a supraphysiological level
of IAA (10 mg L−1). Growth characteristics, total photosynthetic pigment content, histochemical
localization of reactive oxygen species and antioxidant enzyme activity (SOD, CAT and POX) were
evaluated at two time points, after 3 and 7 days of co-cultivation. Superoxide anion and hydrogen
peroxide were produced and accumulated mainly in the roots, daughter fronds and veins of duckweeds.
Duckweeds’ responses depended on the strain of Pseudomonas, time and exogenous IAA.
Co-cultivation of duckweed with bacteria has positive or neutral effects. Exogenous application
of IAA had a negative or neutral effect on enzyme activity and other parameters. Co-cultivation
with P. gessardii C31-106/3 showed plant-growth-promoting effects on duckweed: increased biomass
production, modulation of duckweeds’ antioxidant enzymatic activity and reduction in hydrogen
peroxide content. This study widens our knowledge of aquatic plants and their response to oxidative
stress, supports the hypothesis that plant growth-promoting bacteria (PGPB) induce tolerable levels
of oxidative stress in plants and introduces a new PGPB strain, P. gessardii C31-106/3.",
publisher = "Basel: MDPI",
journal = "Horticulturae",
title = "Antioxidative Response of Duckweed (Lemna minor L.) to Rhizosphere-Associated Pseudomonas Strains and Exogenous Indole-3-Acetic Acid",
number = "6",
volume = "10",
doi = "10.3390/horticulturae10060562",
pages = "562"
}

Popržen, T., Jevremović, S., Milošević, S., Đurić, M., Uzelac, B., Stanković, S.,& Radulović, O.. (2024). Antioxidative Response of Duckweed (Lemna minor L.) to Rhizosphere-Associated Pseudomonas Strains and Exogenous Indole-3-Acetic Acid. in Horticulturae
Basel: MDPI., 10(6), 562.
https://doi.org/10.3390/horticulturae10060562

Popržen T, Jevremović S, Milošević S, Đurić M, Uzelac B, Stanković S, Radulović O. Antioxidative Response of Duckweed (Lemna minor L.) to Rhizosphere-Associated Pseudomonas Strains and Exogenous Indole-3-Acetic Acid. in Horticulturae. 2024;10(6):562.
doi:10.3390/horticulturae10060562 .

Popržen, Tatjana, Jevremović, Slađana, Milošević, Snežana, Đurić, Marija, Uzelac, Branka, Stanković, Slaviša, Radulović, Olga, "Antioxidative Response of Duckweed (Lemna minor L.) to Rhizosphere-Associated Pseudomonas Strains and Exogenous Indole-3-Acetic Acid" in Horticulturae, 10, no. 6 (2024):562,
https://doi.org/10.3390/horticulturae10060562 . .

TY  - JOUR
AU  - Dinić, Jelena
AU  - Dragoj, Miodrag
AU  - Jovanović Stojanov, Sofija
AU  - Stepanović, Ana
AU  - Lupšić, Ema
AU  - Pajović, Milica
AU  - Mohr, Thomas
AU  - Glumac, Sofija
AU  - Marić, Dragana
AU  - Ercegovac, Maja
AU  - Podolski-Renić, Ana
AU  - Pešić, Milica
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6763
AB  - The impact of tyrosine kinase inhibitors (TKIs) on multidrug resistance (MDR) in non-small cell lung carcinoma (NSCLC) is a critical aspect of cancer therapy. While TKIs effectively target specific signaling pathways of cancer cells, they can also act as substrates for ABC transporters, potentially triggering MDR. The aim of our study was to evaluate the response of 17 patient-derived NSCLC cultures to 10 commonly prescribed TKIs and to correlate these responses with patient mutational profiles. Using an ex vivo immunofluorescence assay, we analyzed the expression of the MDR markers ABCB1, ABCC1, and ABCG2, and correlated these data with the genetic profiles of patients for a functional diagnostic approach. NSCLC cultures responded differently to TKIs, with erlotinib showing good efficacy regardless of mutation burden or EGFR status. However, the modulation of MDR mechanisms by erlotinib, such as increased ABCG2 expression, highlights the challenges associated with erlotinib treatment. Other TKIs showed limited efficacy, highlighting the variability of response in NSCLC. Genetic alterations in signaling pathways associated with drug resistance and sensitivity, including TP53 mutations, likely contributed to the variable responses to TKIs. The relationships between ABC transporter expression, gene alterations, and response to TKIs did not show consistent patterns. Our results suggest that in addition to mutational status, performing functional sensitivity screening is critical for identifying appropriate treatment strategies with TKIs. These results underscore the importance of considering drug sensitivity, off-target effects, MDR risks, and patient-specific genetic profiles when optimizing NSCLC treatment and highlight the potential for personalized approaches, especially in early stages.
PB  - Basel: MDPI
T2  - Cancers
T1  - Multidrug-Resistant Profiles in Non-Small Cell Lung Carcinoma Patient-Derived Cells: Implications for Personalized Approaches with Tyrosine Kinase Inhibitors
IS  - 11
VL  - 16
DO  - 10.3390/cancers16111984
SP  - 1984
ER  - 

@article{
author = "Dinić, Jelena and Dragoj, Miodrag and Jovanović Stojanov, Sofija and Stepanović, Ana and Lupšić, Ema and Pajović, Milica and Mohr, Thomas and Glumac, Sofija and Marić, Dragana and Ercegovac, Maja and Podolski-Renić, Ana and Pešić, Milica",
year = "2024",
abstract = "The impact of tyrosine kinase inhibitors (TKIs) on multidrug resistance (MDR) in non-small cell lung carcinoma (NSCLC) is a critical aspect of cancer therapy. While TKIs effectively target specific signaling pathways of cancer cells, they can also act as substrates for ABC transporters, potentially triggering MDR. The aim of our study was to evaluate the response of 17 patient-derived NSCLC cultures to 10 commonly prescribed TKIs and to correlate these responses with patient mutational profiles. Using an ex vivo immunofluorescence assay, we analyzed the expression of the MDR markers ABCB1, ABCC1, and ABCG2, and correlated these data with the genetic profiles of patients for a functional diagnostic approach. NSCLC cultures responded differently to TKIs, with erlotinib showing good efficacy regardless of mutation burden or EGFR status. However, the modulation of MDR mechanisms by erlotinib, such as increased ABCG2 expression, highlights the challenges associated with erlotinib treatment. Other TKIs showed limited efficacy, highlighting the variability of response in NSCLC. Genetic alterations in signaling pathways associated with drug resistance and sensitivity, including TP53 mutations, likely contributed to the variable responses to TKIs. The relationships between ABC transporter expression, gene alterations, and response to TKIs did not show consistent patterns. Our results suggest that in addition to mutational status, performing functional sensitivity screening is critical for identifying appropriate treatment strategies with TKIs. These results underscore the importance of considering drug sensitivity, off-target effects, MDR risks, and patient-specific genetic profiles when optimizing NSCLC treatment and highlight the potential for personalized approaches, especially in early stages.",
publisher = "Basel: MDPI",
journal = "Cancers",
title = "Multidrug-Resistant Profiles in Non-Small Cell Lung Carcinoma Patient-Derived Cells: Implications for Personalized Approaches with Tyrosine Kinase Inhibitors",
number = "11",
volume = "16",
doi = "10.3390/cancers16111984",
pages = "1984"
}

Dinić, J., Dragoj, M., Jovanović Stojanov, S., Stepanović, A., Lupšić, E., Pajović, M., Mohr, T., Glumac, S., Marić, D., Ercegovac, M., Podolski-Renić, A.,& Pešić, M.. (2024). Multidrug-Resistant Profiles in Non-Small Cell Lung Carcinoma Patient-Derived Cells: Implications for Personalized Approaches with Tyrosine Kinase Inhibitors. in Cancers
Basel: MDPI., 16(11), 1984.
https://doi.org/10.3390/cancers16111984

Dinić J, Dragoj M, Jovanović Stojanov S, Stepanović A, Lupšić E, Pajović M, Mohr T, Glumac S, Marić D, Ercegovac M, Podolski-Renić A, Pešić M. Multidrug-Resistant Profiles in Non-Small Cell Lung Carcinoma Patient-Derived Cells: Implications for Personalized Approaches with Tyrosine Kinase Inhibitors. in Cancers. 2024;16(11):1984.
doi:10.3390/cancers16111984 .

Dinić, Jelena, Dragoj, Miodrag, Jovanović Stojanov, Sofija, Stepanović, Ana, Lupšić, Ema, Pajović, Milica, Mohr, Thomas, Glumac, Sofija, Marić, Dragana, Ercegovac, Maja, Podolski-Renić, Ana, Pešić, Milica, "Multidrug-Resistant Profiles in Non-Small Cell Lung Carcinoma Patient-Derived Cells: Implications for Personalized Approaches with Tyrosine Kinase Inhibitors" in Cancers, 16, no. 11 (2024):1984,
https://doi.org/10.3390/cancers16111984 . .

TY  - JOUR
AU  - Bjelica, Vukašin
AU  - Anđelković, Marko
AU  - Maričić, Marko
AU  - Tomović, Ljiljana
AU  - Bonnet, Xavier
AU  - Golubović, Ana
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6756
AB  - When captured, many prey respond by biting the predator or struggling to get free.
However, one indirect escape option is death feigning (DF), in which complete
immobility supposedly “tricks” the predator into abandoning its meal. But lying
motionless and exposed to a predator is dangerous; therefore, individuals should
optimize DF occurrence and duration. We captured colour polymorphic dice snakes
(Natrix tessellata, Natricidae) (N = 271) in the field and measured two behavioural
responses: (1) the occurrence and duration of immobility/DF; (2) the number of
tongue-flicking sequences (TF). Tongue flicking is an essential component of a key
sensory mechanism to check the safety of the environment before attempting
escape. We experimentally assessed the relationship between these two behaviours
and the effects of phenotypic characteristic of snakes on the occurrence and duration
of immobility and of TF. Snake phenotype had multiple effects. Gravid
females avoided DF and displayed more TF sequences during the tests compared
to non-gravid females and males. Blotched snakes stayed immobile longer than
green and melanistic snakes. Larger individuals remained in DF for longer and
showed fewer TF sequences than smaller individuals. Snakes burdened with a
recent meal postponed fleeing and displayed more TF sequences than snakes without
food. Finally, snakes showing more TF sequences postponed fleeing, which
suggests that dice snakes assessed predatory risks and adapted escape behaviour to
their risk status. Future studies should examine how individuals estimate the appropriate
timing to shift from immobility to escape.
PB  - Hoboken: Wiley
T2  - Journal of Zoology
T1  - Tongue flicking heralds flight behaviour following passive antipredator displays in dice snakes
IS  - 4
VL  - 322
DO  - 10.1111/jzo.13150
SP  - 364
EP  - 374
ER  - 

@article{
author = "Bjelica, Vukašin and Anđelković, Marko and Maričić, Marko and Tomović, Ljiljana and Bonnet, Xavier and Golubović, Ana",
year = "2024",
abstract = "When captured, many prey respond by biting the predator or struggling to get free.
However, one indirect escape option is death feigning (DF), in which complete
immobility supposedly “tricks” the predator into abandoning its meal. But lying
motionless and exposed to a predator is dangerous; therefore, individuals should
optimize DF occurrence and duration. We captured colour polymorphic dice snakes
(Natrix tessellata, Natricidae) (N = 271) in the field and measured two behavioural
responses: (1) the occurrence and duration of immobility/DF; (2) the number of
tongue-flicking sequences (TF). Tongue flicking is an essential component of a key
sensory mechanism to check the safety of the environment before attempting
escape. We experimentally assessed the relationship between these two behaviours
and the effects of phenotypic characteristic of snakes on the occurrence and duration
of immobility and of TF. Snake phenotype had multiple effects. Gravid
females avoided DF and displayed more TF sequences during the tests compared
to non-gravid females and males. Blotched snakes stayed immobile longer than
green and melanistic snakes. Larger individuals remained in DF for longer and
showed fewer TF sequences than smaller individuals. Snakes burdened with a
recent meal postponed fleeing and displayed more TF sequences than snakes without
food. Finally, snakes showing more TF sequences postponed fleeing, which
suggests that dice snakes assessed predatory risks and adapted escape behaviour to
their risk status. Future studies should examine how individuals estimate the appropriate
timing to shift from immobility to escape.",
publisher = "Hoboken: Wiley",
journal = "Journal of Zoology",
title = "Tongue flicking heralds flight behaviour following passive antipredator displays in dice snakes",
number = "4",
volume = "322",
doi = "10.1111/jzo.13150",
pages = "364-374"
}

Bjelica, V., Anđelković, M., Maričić, M., Tomović, L., Bonnet, X.,& Golubović, A.. (2024). Tongue flicking heralds flight behaviour following passive antipredator displays in dice snakes. in Journal of Zoology
Hoboken: Wiley., 322(4), 364-374.
https://doi.org/10.1111/jzo.13150

Bjelica V, Anđelković M, Maričić M, Tomović L, Bonnet X, Golubović A. Tongue flicking heralds flight behaviour following passive antipredator displays in dice snakes. in Journal of Zoology. 2024;322(4):364-374.
doi:10.1111/jzo.13150 .

Bjelica, Vukašin, Anđelković, Marko, Maričić, Marko, Tomović, Ljiljana, Bonnet, Xavier, Golubović, Ana, "Tongue flicking heralds flight behaviour following passive antipredator displays in dice snakes" in Journal of Zoology, 322, no. 4 (2024):364-374,
https://doi.org/10.1111/jzo.13150 . .

TY  - JOUR
AU  - Šaponjić, Jasna
AU  - Mejías, Rebeca
AU  - Nikolovski, Neda
AU  - Dragic, Milorad
AU  - Canak, Asuman
AU  - Papoutsopoulou, Stamatia
AU  - Gürsoy-Özdemir, Yasemin
AU  - Fladmark, Kari
AU  - Ntavaroukas, Panagiotis
AU  - Bayar Muluk, Nuray
AU  - Zeljkovic Jovanovic, Milica
AU  - Fontán-Lozano, Ángela
AU  - Comi, Cristoforo
AU  - Marino, Franca
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6685
AB  - Parkinson’s disease (PD) is a chronic, age-related, progressive multisystem disease associated with neuroinflammation and immune dysfunction. This review discusses the methodological
approaches used to study the changes in central and peripheral immunity in PD, the advantages and
limitations of the techniques, and their applicability to humans. Although a single animal model
cannot replicate all pathological features of the human disease, neuroinflammation is present in
most animal models of PD and plays a critical role in understanding the involvement of the immune
system (IS) in the pathogenesis of PD. The IS and its interactions with different cell types in the
central nervous system (CNS) play an important role in the pathogenesis of PD. Even though culture
models do not fully reflect the complexity of disease progression, they are limited in their ability to
mimic long-term effects and need validation through in vivo studies. They are an indispensable tool
for understanding the interplay between the IS and the pathogenesis of this disease. Understanding
the immune-mediated mechanisms may lead to potential therapeutic targets for the treatment of PD.
We believe that the development of methodological guidelines for experiments with animal models
and PD patients is crucial to ensure the validity and consistency of the results.
PB  - Basel: MDPI
T2  - International Journal of Molecular Sciences
T1  - Experimental Models to Study Immune Dysfunction in the Pathogenesis of Parkinson’s Disease
IS  - 8
VL  - 25
DO  - 10.3390/ijms25084330
SP  - 4330
ER  - 

@article{
author = "Šaponjić, Jasna and Mejías, Rebeca and Nikolovski, Neda and Dragic, Milorad and Canak, Asuman and Papoutsopoulou, Stamatia and Gürsoy-Özdemir, Yasemin and Fladmark, Kari and Ntavaroukas, Panagiotis and Bayar Muluk, Nuray and Zeljkovic Jovanovic, Milica and Fontán-Lozano, Ángela and Comi, Cristoforo and Marino, Franca",
year = "2024",
abstract = "Parkinson’s disease (PD) is a chronic, age-related, progressive multisystem disease associated with neuroinflammation and immune dysfunction. This review discusses the methodological
approaches used to study the changes in central and peripheral immunity in PD, the advantages and
limitations of the techniques, and their applicability to humans. Although a single animal model
cannot replicate all pathological features of the human disease, neuroinflammation is present in
most animal models of PD and plays a critical role in understanding the involvement of the immune
system (IS) in the pathogenesis of PD. The IS and its interactions with different cell types in the
central nervous system (CNS) play an important role in the pathogenesis of PD. Even though culture
models do not fully reflect the complexity of disease progression, they are limited in their ability to
mimic long-term effects and need validation through in vivo studies. They are an indispensable tool
for understanding the interplay between the IS and the pathogenesis of this disease. Understanding
the immune-mediated mechanisms may lead to potential therapeutic targets for the treatment of PD.
We believe that the development of methodological guidelines for experiments with animal models
and PD patients is crucial to ensure the validity and consistency of the results.",
publisher = "Basel: MDPI",
journal = "International Journal of Molecular Sciences",
title = "Experimental Models to Study Immune Dysfunction in the Pathogenesis of Parkinson’s Disease",
number = "8",
volume = "25",
doi = "10.3390/ijms25084330",
pages = "4330"
}

Šaponjić, J., Mejías, R., Nikolovski, N., Dragic, M., Canak, A., Papoutsopoulou, S., Gürsoy-Özdemir, Y., Fladmark, K., Ntavaroukas, P., Bayar Muluk, N., Zeljkovic Jovanovic, M., Fontán-Lozano, Á., Comi, C.,& Marino, F.. (2024). Experimental Models to Study Immune Dysfunction in the Pathogenesis of Parkinson’s Disease. in International Journal of Molecular Sciences
Basel: MDPI., 25(8), 4330.
https://doi.org/10.3390/ijms25084330

Šaponjić J, Mejías R, Nikolovski N, Dragic M, Canak A, Papoutsopoulou S, Gürsoy-Özdemir Y, Fladmark K, Ntavaroukas P, Bayar Muluk N, Zeljkovic Jovanovic M, Fontán-Lozano Á, Comi C, Marino F. Experimental Models to Study Immune Dysfunction in the Pathogenesis of Parkinson’s Disease. in International Journal of Molecular Sciences. 2024;25(8):4330.
doi:10.3390/ijms25084330 .

Šaponjić, Jasna, Mejías, Rebeca, Nikolovski, Neda, Dragic, Milorad, Canak, Asuman, Papoutsopoulou, Stamatia, Gürsoy-Özdemir, Yasemin, Fladmark, Kari, Ntavaroukas, Panagiotis, Bayar Muluk, Nuray, Zeljkovic Jovanovic, Milica, Fontán-Lozano, Ángela, Comi, Cristoforo, Marino, Franca, "Experimental Models to Study Immune Dysfunction in the Pathogenesis of Parkinson’s Disease" in International Journal of Molecular Sciences, 25, no. 8 (2024):4330,
https://doi.org/10.3390/ijms25084330 . .

TY  - JOUR
AU  - Aničić, Neda
AU  - Mišić, Danijela
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6552
AB  - The genus Nepeta, belonging to the Lamiaceae family, encompasses a diverse group of plants with significant
biological activities attributed mainly to their iridoid compounds. This review provides a comprehensive analysis
of recent research on iridoid biosynthesis, regulation, and evolutionary aspects within the Nepeta genus. The biological
activities of Nepeta species, including repellent, phytotoxic, antimicrobial, and cytotoxic effects, have been extensively
investigated, highlighting the potential applications of iridoids. Over the past decade, significant progress has been
made in elucidating the molecular basis of iridoid biosynthesis and regulation, thanks to advancements in transcriptomics,
genomics, and metabolomics. The presence of distinct chemotype groups within Nepeta has been revealed,
characterized by their ability to produce both iridoid aglycones (nepetalactones) and glycosylated iridoids (IAs and
IGs), exclusively produce IGs, or lack iridoids. The identification of key enzymes involved in iridoid biosynthesis, such
as geraniol synthase (GES) and iridoid synthase (ISY), has played a crucial role in understanding the pathway. Furthermore,
the evolutionary aspects of the iridoid biosynthesis loss in some of the Nepeta taxa, and the association of iridoid
presence and content with the expression levels of specific genes, have been investigated. However, several areas remain
to be explored, including the final steps of iridoid aglycones biosynthetic branch, the production of iridoid glucosides,
the role of transcription factors in fine-tuning of iridoid biosynthesis, and the intricate interplay between biosynthetic
enzymes. Continued research in these areas will deepen our understanding of iridoid metabolism in Nepeta and unlock
their full potential in various fields, including pharmaceuticals, agriculture, and natural product-based industries.
PB  - Novi Sad, Serbia: Biologia Serbica
T2  - Biologia Serbica
T1  - Unveiling the evolution of iridoid biosynthesis in the genus Nepeta: a mini review
VL  - 46
DO  - 10.5281/zenodo.10606764
ER  - 

@article{
author = "Aničić, Neda and Mišić, Danijela",
year = "2024",
abstract = "The genus Nepeta, belonging to the Lamiaceae family, encompasses a diverse group of plants with significant
biological activities attributed mainly to their iridoid compounds. This review provides a comprehensive analysis
of recent research on iridoid biosynthesis, regulation, and evolutionary aspects within the Nepeta genus. The biological
activities of Nepeta species, including repellent, phytotoxic, antimicrobial, and cytotoxic effects, have been extensively
investigated, highlighting the potential applications of iridoids. Over the past decade, significant progress has been
made in elucidating the molecular basis of iridoid biosynthesis and regulation, thanks to advancements in transcriptomics,
genomics, and metabolomics. The presence of distinct chemotype groups within Nepeta has been revealed,
characterized by their ability to produce both iridoid aglycones (nepetalactones) and glycosylated iridoids (IAs and
IGs), exclusively produce IGs, or lack iridoids. The identification of key enzymes involved in iridoid biosynthesis, such
as geraniol synthase (GES) and iridoid synthase (ISY), has played a crucial role in understanding the pathway. Furthermore,
the evolutionary aspects of the iridoid biosynthesis loss in some of the Nepeta taxa, and the association of iridoid
presence and content with the expression levels of specific genes, have been investigated. However, several areas remain
to be explored, including the final steps of iridoid aglycones biosynthetic branch, the production of iridoid glucosides,
the role of transcription factors in fine-tuning of iridoid biosynthesis, and the intricate interplay between biosynthetic
enzymes. Continued research in these areas will deepen our understanding of iridoid metabolism in Nepeta and unlock
their full potential in various fields, including pharmaceuticals, agriculture, and natural product-based industries.",
publisher = "Novi Sad, Serbia: Biologia Serbica",
journal = "Biologia Serbica",
title = "Unveiling the evolution of iridoid biosynthesis in the genus Nepeta: a mini review",
volume = "46",
doi = "10.5281/zenodo.10606764"
}

Aničić, N.,& Mišić, D.. (2024). Unveiling the evolution of iridoid biosynthesis in the genus Nepeta: a mini review. in Biologia Serbica
Novi Sad, Serbia: Biologia Serbica., 46.
https://doi.org/10.5281/zenodo.10606764

Aničić N, Mišić D. Unveiling the evolution of iridoid biosynthesis in the genus Nepeta: a mini review. in Biologia Serbica. 2024;46.
doi:10.5281/zenodo.10606764 .

Aničić, Neda, Mišić, Danijela, "Unveiling the evolution of iridoid biosynthesis in the genus Nepeta: a mini review" in Biologia Serbica, 46 (2024),
https://doi.org/10.5281/zenodo.10606764 . .

TY  - THES
AU  - Erić, Pavle
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6797
AB  - Mitohondrijska DNK (mtDNK) kodira gene uključene u fundamentalne biološke procese ćelijske respiracije i proizvodnje energije. Mitohondrijski genom je haploidan, nasleđuje se uglavnom materinski, gotovo nikad se ne rekombinuje i odlikuje ga relativno mala efektivna veličina populacije. Zbog navedenih odlika mtDNK, uslovi koji predviđaju postojanje i održavanje adaptivne varijabilnosti mtDNK unutar populacija su restriktivni.
Analiziran je adaptivni značaj simpatričke mtDNK varijabilnosti, prisustvo mito-jedarnih interakcija, kao i selekcioni mehanizmi koji je održavaju: polno specifična selekcija, kao i selekcija u varijabilnim sredinskim uslovima. Korišćene su dve široko zastupljene palearktičke vrste roda Drosophila (iz Obscura grupe vrsta), D. subobscura i D. obscura. Prva vrsta je već ustaljeni model u ovom vidu evoluciono genetičkih istraživanja, i odlikuje se prisustvom dva učestala haplotipa u svim populacijama. Druga vrsta je kao model razvijena u ovoj disertaciji. Određena je njena genetička varijabilnost i izučena populaciona istorija kod nekoliko evropskih populacija. Izabrane su linije reprezentativnih haplotipova obe vrste i povratnim ukrštanjima su konstruisane eksperimentalne linije sa svim kombinacijama simpatričkih mtDNK haplotipova i jedarnih pozadina (nuDNK). Na dve eksperimentalne postavke koje su podrazumevale razlike u temperaturi i svetlosnom režimu analizirane su komponente životne istorije svih konstruisanih linija: otpornost na desikaciju, dužina razvića, preadultna vijabilnost, kao i procenat mužjaka (odnos polova).
Rezultati su pokazali postojanje značajne genetičke varijabilnosti kako unutar tako i između populacija D. obscura. Kod ove vrste je uočena geografska struktuiranost i smanjenje genetičkog diverziteta u pravcu od zapada ka istoku. Pokazan je adaptivni značaj simpatričke mtDNK varijabilnosti obe vrste. Kod D. subobscura je on bio prisutan na nivou dva glavna haplotipa jedino za vijabilitet, a kod ostalih komponenti na nivou pretpostavljenih dodatnih nukleotidnih razlika karakterističnih za pojedinačne linije. Pokazano je da su mito-jedarni genotipovi primarne jedinice na koje deluje prirodna selekcija, a efekat ovih interakcija na fitnes je bio izraženiji kod D. obscura. Pečat polno specifične selekcije nije zabeležen kod D. subobscura, dok je kod D. obscura efekat ovog vida balansne selekcije bio umeren. Naposletku, eksperimenti su pokazali umeren efekat interakcija sredine i mito-jedarnog genotipa na fitnes kod D. subobscura, dok su kod D. obscura pokazali jači uticaj ovog vida balansne selekcije. Uz to kod D. obscura primećen je i efekat interakcija samog mitohondrijskog haplotipa i sredinskih faktora na fitnes jedinki. Posmatrajući obe vrste, analizirana sredinska varijabilnost se pokazala važnijom u održavanju mtDNK varijabilnosti u poređenju sa polno specifičnom selekcijom.
Upotreba dve vrste sa različitim obrascima mtDNK varijabilnosti omogućila je uvid u adaptivne razlike na barem dva različita stupnja funkcionalne povezanosti mitohondrijskog i jedarnog genoma. Mali je broj eksperimentalnih radova koji ispituju mehanizme održavanja simpatričke varijabilnosti mtDNK, a rezultati ove studije umnogome doprinose njihovom boljem razumevanju.
AB  - Mitochondrial DNA (mtDNA) encodes genes involved in fundamental biological processes of cellular respiration and energy production. The mitochondrial genome is haploid, usually maternally inherited, rarely undergoes recombination, and is characterised by a relatively small effective population size. Due to these characteristics of mtDNA, conditions predicting the existence and maintenance of adaptive mtDNA variation within populations are restrictive.
The adaptive significance of sympatric mtDNA variability, the presence of mito-nuclear interactions, and selective mechanisms maintaining it were analysed, including sex-specific selection and selection in variable environmental conditions. Two widely distributed Palearctic species from the Drosophila genus (Obscura group species), D. subobscura and D. obscura, were used. The first species, a well-established model in evolutionary genetic research, features two common haplotypes in all populations. The second species was developed as a model in this dissertation. Genetic variability and population history of several European populations were determined as part of this thesis. Lines of representative haplotypes of both species were selected, and experimental lines with all combinations of sympatric mtDNA haplotypes and nuclear backgrounds (nuDNA) were constructed through backcrossing. All constructed lines of both species were subjected to life history experiments: desiccation resistance, developmental time, preadult viability, and sex ratio. Life history assays were conducted with two experimental setups involving differences in temperature and light regime.
The results showed significant genetic variability both within and between populations of D. obscura. In this species, geographic structuring and a reduction in genetic diversity from west to east was observed. The adaptive significance of sympatric mtDNA variation was demonstrated in both species. In D. subobscura, this significance was not present at the level of two main haplotypes except in viability experiment, and in other components it was assumed to involve additional, line specific nucleotide differences. Mito-nuclear genotypes were identified as primary units affected by natural selection, with a more pronounced effect of these interactions on fitness observed in D. obscura. Regarding sex-specific selection, its impact was not observed in D. subobscura, while in D. obscura, the effect of this type of balancing selection was moderate. Finally, experiments showed a moderate effect of interactions between the environment and mito-nuclear genotype on fitness in D. subobscura, while in D. obscura showed a stronger influence of this type of balancing selection. Additionally, in D. obscura, an effect of interactions between the mitochondrial haplotype itself and environmental factors on the fitness of individuals was observed. Considering both species, environmental variability proved more important in maintaining mtDNA variation compared to sex-specific selection.
The use of two species with different patterns of mtDNA variability provided insight into adaptive differences at least two different levels of functional connectivity between the mitochondrial and nuclear genomes. Few experimental studies have examined the mechanisms sustaining sympatric mtDNA variability, and the results of this study significantly contribute to a better understanding of these mechanisms.
PB  - Belgrade: University of Belgrade, Faculty of Biology
T2  - University of Belgrade, Faculty of Biology
T1  - Adaptivni značaj unutarpopulacione varijabilnosti mitohondrijskog genoma Drosophila subobscura i D. obscura
T1  - Adaptive significance of intrapopulational mitochondrial DNA variation in Drosophila subobscura and D. obscura
SP  - 1
EP  - 156
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6797
ER  - 

@phdthesis{
author = "Erić, Pavle",
year = "2024",
abstract = "Mitohondrijska DNK (mtDNK) kodira gene uključene u fundamentalne biološke procese ćelijske respiracije i proizvodnje energije. Mitohondrijski genom je haploidan, nasleđuje se uglavnom materinski, gotovo nikad se ne rekombinuje i odlikuje ga relativno mala efektivna veličina populacije. Zbog navedenih odlika mtDNK, uslovi koji predviđaju postojanje i održavanje adaptivne varijabilnosti mtDNK unutar populacija su restriktivni.
Analiziran je adaptivni značaj simpatričke mtDNK varijabilnosti, prisustvo mito-jedarnih interakcija, kao i selekcioni mehanizmi koji je održavaju: polno specifična selekcija, kao i selekcija u varijabilnim sredinskim uslovima. Korišćene su dve široko zastupljene palearktičke vrste roda Drosophila (iz Obscura grupe vrsta), D. subobscura i D. obscura. Prva vrsta je već ustaljeni model u ovom vidu evoluciono genetičkih istraživanja, i odlikuje se prisustvom dva učestala haplotipa u svim populacijama. Druga vrsta je kao model razvijena u ovoj disertaciji. Određena je njena genetička varijabilnost i izučena populaciona istorija kod nekoliko evropskih populacija. Izabrane su linije reprezentativnih haplotipova obe vrste i povratnim ukrštanjima su konstruisane eksperimentalne linije sa svim kombinacijama simpatričkih mtDNK haplotipova i jedarnih pozadina (nuDNK). Na dve eksperimentalne postavke koje su podrazumevale razlike u temperaturi i svetlosnom režimu analizirane su komponente životne istorije svih konstruisanih linija: otpornost na desikaciju, dužina razvića, preadultna vijabilnost, kao i procenat mužjaka (odnos polova).
Rezultati su pokazali postojanje značajne genetičke varijabilnosti kako unutar tako i između populacija D. obscura. Kod ove vrste je uočena geografska struktuiranost i smanjenje genetičkog diverziteta u pravcu od zapada ka istoku. Pokazan je adaptivni značaj simpatričke mtDNK varijabilnosti obe vrste. Kod D. subobscura je on bio prisutan na nivou dva glavna haplotipa jedino za vijabilitet, a kod ostalih komponenti na nivou pretpostavljenih dodatnih nukleotidnih razlika karakterističnih za pojedinačne linije. Pokazano je da su mito-jedarni genotipovi primarne jedinice na koje deluje prirodna selekcija, a efekat ovih interakcija na fitnes je bio izraženiji kod D. obscura. Pečat polno specifične selekcije nije zabeležen kod D. subobscura, dok je kod D. obscura efekat ovog vida balansne selekcije bio umeren. Naposletku, eksperimenti su pokazali umeren efekat interakcija sredine i mito-jedarnog genotipa na fitnes kod D. subobscura, dok su kod D. obscura pokazali jači uticaj ovog vida balansne selekcije. Uz to kod D. obscura primećen je i efekat interakcija samog mitohondrijskog haplotipa i sredinskih faktora na fitnes jedinki. Posmatrajući obe vrste, analizirana sredinska varijabilnost se pokazala važnijom u održavanju mtDNK varijabilnosti u poređenju sa polno specifičnom selekcijom.
Upotreba dve vrste sa različitim obrascima mtDNK varijabilnosti omogućila je uvid u adaptivne razlike na barem dva različita stupnja funkcionalne povezanosti mitohondrijskog i jedarnog genoma. Mali je broj eksperimentalnih radova koji ispituju mehanizme održavanja simpatričke varijabilnosti mtDNK, a rezultati ove studije umnogome doprinose njihovom boljem razumevanju., Mitochondrial DNA (mtDNA) encodes genes involved in fundamental biological processes of cellular respiration and energy production. The mitochondrial genome is haploid, usually maternally inherited, rarely undergoes recombination, and is characterised by a relatively small effective population size. Due to these characteristics of mtDNA, conditions predicting the existence and maintenance of adaptive mtDNA variation within populations are restrictive.
The adaptive significance of sympatric mtDNA variability, the presence of mito-nuclear interactions, and selective mechanisms maintaining it were analysed, including sex-specific selection and selection in variable environmental conditions. Two widely distributed Palearctic species from the Drosophila genus (Obscura group species), D. subobscura and D. obscura, were used. The first species, a well-established model in evolutionary genetic research, features two common haplotypes in all populations. The second species was developed as a model in this dissertation. Genetic variability and population history of several European populations were determined as part of this thesis. Lines of representative haplotypes of both species were selected, and experimental lines with all combinations of sympatric mtDNA haplotypes and nuclear backgrounds (nuDNA) were constructed through backcrossing. All constructed lines of both species were subjected to life history experiments: desiccation resistance, developmental time, preadult viability, and sex ratio. Life history assays were conducted with two experimental setups involving differences in temperature and light regime.
The results showed significant genetic variability both within and between populations of D. obscura. In this species, geographic structuring and a reduction in genetic diversity from west to east was observed. The adaptive significance of sympatric mtDNA variation was demonstrated in both species. In D. subobscura, this significance was not present at the level of two main haplotypes except in viability experiment, and in other components it was assumed to involve additional, line specific nucleotide differences. Mito-nuclear genotypes were identified as primary units affected by natural selection, with a more pronounced effect of these interactions on fitness observed in D. obscura. Regarding sex-specific selection, its impact was not observed in D. subobscura, while in D. obscura, the effect of this type of balancing selection was moderate. Finally, experiments showed a moderate effect of interactions between the environment and mito-nuclear genotype on fitness in D. subobscura, while in D. obscura showed a stronger influence of this type of balancing selection. Additionally, in D. obscura, an effect of interactions between the mitochondrial haplotype itself and environmental factors on the fitness of individuals was observed. Considering both species, environmental variability proved more important in maintaining mtDNA variation compared to sex-specific selection.
The use of two species with different patterns of mtDNA variability provided insight into adaptive differences at least two different levels of functional connectivity between the mitochondrial and nuclear genomes. Few experimental studies have examined the mechanisms sustaining sympatric mtDNA variability, and the results of this study significantly contribute to a better understanding of these mechanisms.",
publisher = "Belgrade: University of Belgrade, Faculty of Biology",
journal = "University of Belgrade, Faculty of Biology",
title = "Adaptivni značaj unutarpopulacione varijabilnosti mitohondrijskog genoma Drosophila subobscura i D. obscura, Adaptive significance of intrapopulational mitochondrial DNA variation in Drosophila subobscura and D. obscura",
pages = "1-156",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6797"
}

Erić, P.. (2024). Adaptivni značaj unutarpopulacione varijabilnosti mitohondrijskog genoma Drosophila subobscura i D. obscura. in University of Belgrade, Faculty of Biology
Belgrade: University of Belgrade, Faculty of Biology., 1-156.
https://hdl.handle.net/21.15107/rcub_ibiss_6797

Erić P. Adaptivni značaj unutarpopulacione varijabilnosti mitohondrijskog genoma Drosophila subobscura i D. obscura. in University of Belgrade, Faculty of Biology. 2024;:1-156.
https://hdl.handle.net/21.15107/rcub_ibiss_6797 .

Erić, Pavle, "Adaptivni značaj unutarpopulacione varijabilnosti mitohondrijskog genoma Drosophila subobscura i D. obscura" in University of Belgrade, Faculty of Biology (2024):1-156,
https://hdl.handle.net/21.15107/rcub_ibiss_6797 .

TY  - DATA
AU  - Budečević, Sanja
AU  - Predojević, Dragana
AU  - Đorđević, Mirko
AU  - Vlajnić, Lea
AU  - Stojković, Oliver
AU  - Stojković, Biljana
AU  - Savković, Uroš
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6730
AB  - This file contains data for the morphological analyses (landmarks coordinates) of  four populations of seed beetle, Acanthoscelides obtectus exposed to sub-lethal doses of cypermethrin for ten generations in a laboratory evolution experiment. This file is in the .csv format and it contains one sheet and forty nine columns.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković" – National Institute of Republic of Serbia, University of Belgrade
T1  - Data for Transgenerational sub-lethal pyrethroid exposure affects shape variation and fluctuating asymmetry in a seed beetle
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6730
ER  - 

@misc{
author = "Budečević, Sanja and Predojević, Dragana and Đorđević, Mirko and Vlajnić, Lea and Stojković, Oliver and Stojković, Biljana and Savković, Uroš",
year = "2024",
abstract = "This file contains data for the morphological analyses (landmarks coordinates) of  four populations of seed beetle, Acanthoscelides obtectus exposed to sub-lethal doses of cypermethrin for ten generations in a laboratory evolution experiment. This file is in the .csv format and it contains one sheet and forty nine columns.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković" – National Institute of Republic of Serbia, University of Belgrade",
title = "Data for Transgenerational sub-lethal pyrethroid exposure affects shape variation and fluctuating asymmetry in a seed beetle",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6730"
}

Budečević, S., Predojević, D., Đorđević, M., Vlajnić, L., Stojković, O., Stojković, B.,& Savković, U.. (2024). Data for Transgenerational sub-lethal pyrethroid exposure affects shape variation and fluctuating asymmetry in a seed beetle. 
Belgrade: Institute for Biological Research "Siniša Stanković" – National Institute of Republic of Serbia, University of Belgrade..
https://hdl.handle.net/21.15107/rcub_ibiss_6730

Budečević S, Predojević D, Đorđević M, Vlajnić L, Stojković O, Stojković B, Savković U. Data for Transgenerational sub-lethal pyrethroid exposure affects shape variation and fluctuating asymmetry in a seed beetle. 2024;.
https://hdl.handle.net/21.15107/rcub_ibiss_6730 .

Budečević, Sanja, Predojević, Dragana, Đorđević, Mirko, Vlajnić, Lea, Stojković, Oliver, Stojković, Biljana, Savković, Uroš, "Data for Transgenerational sub-lethal pyrethroid exposure affects shape variation and fluctuating asymmetry in a seed beetle" (2024),
https://hdl.handle.net/21.15107/rcub_ibiss_6730 .

TY  - CONF
AU  - Zdravković-Korać, Snežana
AU  - Ćalić, Dušica
AU  - Milojević, Jelena
AU  - Belić, Maja
AU  - Jevremović, Slađana
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6713
AB  - Aesculus species are valuable ornamental plants, while their seeds are used as a source of health-promoting aescin. The somatic embryos (SE) of A. hippocastanum (horse chestnut) contain aescin in similar amounts as the seeds. Different pathways of propagation by tissue culture for several Aesculus species (A. hippocastanum, A. flava and A. carnea) have been investigated for few decades in our laboratory. Recently, an efficient protocol for somatic embryogenesis induction in stamen filaments culture has been developed. However, the frequency of embryogenic tissue (ET) induction varied markedly (0–50%) for the same genotypes from year to year. In A. flava, a significant increase (80–100%) in the frequency of ET induction was achieved by using liquid culture of friable calli. After the establishment of stable liquid cultures, lines with high ET proliferation and SE development were selected. Significant fresh weight increase (76–167-fold), with 256–669 newly developed SEs per 100 mg of initial inoculum, were achieved four weeks after plating on solid medium. In addition, most SEs (19–39%) reached the late torpedo and cotyledonary stage of development. SEs have high germination rate (80%), but conversion rates still need to be improved to enable sustained clonal propagation of elite specimens. The established protocols for ET induction and SE production represent a clean and safe technology for the in vitro production of Aesculus species, including alternative sources of aescin.
PB  - COST Action CA21157 COPYTREE
C3  - Book of Abstracts: The 2nd Conference of COST Action CA21157 COPYTREE: In vitro culture of woody crops: problem solving by new approaches; 2024 Apr 22-24; Jūrmala, Latvia
T1  - Recent advances in somatic embryogenesis induction in Aesculus species
SP  - 38
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6713
ER  - 

@conference{
author = "Zdravković-Korać, Snežana and Ćalić, Dušica and Milojević, Jelena and Belić, Maja and Jevremović, Slađana",
year = "2024",
abstract = "Aesculus species are valuable ornamental plants, while their seeds are used as a source of health-promoting aescin. The somatic embryos (SE) of A. hippocastanum (horse chestnut) contain aescin in similar amounts as the seeds. Different pathways of propagation by tissue culture for several Aesculus species (A. hippocastanum, A. flava and A. carnea) have been investigated for few decades in our laboratory. Recently, an efficient protocol for somatic embryogenesis induction in stamen filaments culture has been developed. However, the frequency of embryogenic tissue (ET) induction varied markedly (0–50%) for the same genotypes from year to year. In A. flava, a significant increase (80–100%) in the frequency of ET induction was achieved by using liquid culture of friable calli. After the establishment of stable liquid cultures, lines with high ET proliferation and SE development were selected. Significant fresh weight increase (76–167-fold), with 256–669 newly developed SEs per 100 mg of initial inoculum, were achieved four weeks after plating on solid medium. In addition, most SEs (19–39%) reached the late torpedo and cotyledonary stage of development. SEs have high germination rate (80%), but conversion rates still need to be improved to enable sustained clonal propagation of elite specimens. The established protocols for ET induction and SE production represent a clean and safe technology for the in vitro production of Aesculus species, including alternative sources of aescin.",
publisher = "COST Action CA21157 COPYTREE",
journal = "Book of Abstracts: The 2nd Conference of COST Action CA21157 COPYTREE: In vitro culture of woody crops: problem solving by new approaches; 2024 Apr 22-24; Jūrmala, Latvia",
title = "Recent advances in somatic embryogenesis induction in Aesculus species",
pages = "38",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6713"
}

Zdravković-Korać, S., Ćalić, D., Milojević, J., Belić, M.,& Jevremović, S.. (2024). Recent advances in somatic embryogenesis induction in Aesculus species. in Book of Abstracts: The 2nd Conference of COST Action CA21157 COPYTREE: In vitro culture of woody crops: problem solving by new approaches; 2024 Apr 22-24; Jūrmala, Latvia
COST Action CA21157 COPYTREE., 38.
https://hdl.handle.net/21.15107/rcub_ibiss_6713

Zdravković-Korać S, Ćalić D, Milojević J, Belić M, Jevremović S. Recent advances in somatic embryogenesis induction in Aesculus species. in Book of Abstracts: The 2nd Conference of COST Action CA21157 COPYTREE: In vitro culture of woody crops: problem solving by new approaches; 2024 Apr 22-24; Jūrmala, Latvia. 2024;:38.
https://hdl.handle.net/21.15107/rcub_ibiss_6713 .

Zdravković-Korać, Snežana, Ćalić, Dušica, Milojević, Jelena, Belić, Maja, Jevremović, Slađana, "Recent advances in somatic embryogenesis induction in Aesculus species" in Book of Abstracts: The 2nd Conference of COST Action CA21157 COPYTREE: In vitro culture of woody crops: problem solving by new approaches; 2024 Apr 22-24; Jūrmala, Latvia (2024):38,
https://hdl.handle.net/21.15107/rcub_ibiss_6713 .

TY  - CONF
AU  - Tovilović-Kovačević, Gordana
AU  - Ignjatović, Đurđica
AU  - Krstić-Milošević, Dijana
AU  - Tomić, Mirko
AU  - Ćosić, Tatjana
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6712
AB  - Thymus pannonicus All. (Lamiaceae) is a perennial plant native to Central and Eastern Europe and Russia. The dried herb is traditionally used to treat mild respiratory and gastrointestinal illnesses, due to enrichment with monoterpenes and sesquiterpenes. Other secondary metabolites found in Thymus species, namely phenolic acids, flavonoids, flavone glucuronides, show numerous additional health-promoting effects (antioxidant, antidiabetic, anticancer). In this work, the antihepatoma activity of methanol extracts from in vitro shoot cultures of T. pannonicus citral chemotype on human HEPG2 hepatoma cells was evaluated. Two methanol extracts were investigated: E1 – derived from shoots grown on a medium without plant growth regulators and E2 – derived from shoots grown in the presence of the plant hormone indole-3-acetic acid (1mg/ml). Quantitative spectrophotometric analysis revealed that total phenolic and flavonoid contents were higher in E2 (540.4mg/g and 9.84mg/g, respectively) than in E1 (265.4mg/g and 6.52mg/g, respectively). Further analysis using HPLC identified rosmarinic acid (RA) as the dominant phenolic acid in both extracts, with E2 containing a slightly higher amount of RA than E1. The extracts reduced growth of HEPG2 cells in a dose- and time-dependent manner with moderate IC50 values (IC50(E1)=81.1µg/ml; IC50(E2)=77.8µg/ml, MTT, 96h), as shown by cell viability tests. Similarly, RA, used as a control, suppressed the growth of HEPG2 cells with an IC50=30.8µM (MTT, 96h). Flow cytometry of propidium iodide-stained cells showed that T. pannonicus extracts and RA arrested HEPG2 cells in the G2/M phase of the cell cycle, while fluorimetric measurement of cells loaded with dihydrorhodamine revealed mild elevation of intracellular ROS content induced by both extracts after 48h of treatment. Considering high content of RA in both extracts and similar antiproliferative effect of RA, E1 and E2 on HEPG2 cells, it could be proposed that RA is the active compound responsible for moderate antihepatoma activity of the investigated T. pannonicus extracts
PB  - Sarajevo: Institute for Genetic Engineering and Biotechnology, University of Sarajevo
C3  - Book of abstracts: 2nd B&H Symposium of Laboratory Geneticists and Molecular Biologists (with International Participation); 2024 May 10-11; Banja Luka, Bosnia and Herzegovina
T1  - Antihepatoma activity of methanol extracts from Thymus pannonicus in vitro shoot cultures
SP  - 27
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6712
ER  - 

@conference{
author = "Tovilović-Kovačević, Gordana and Ignjatović, Đurđica and Krstić-Milošević, Dijana and Tomić, Mirko and Ćosić, Tatjana",
year = "2024",
abstract = "Thymus pannonicus All. (Lamiaceae) is a perennial plant native to Central and Eastern Europe and Russia. The dried herb is traditionally used to treat mild respiratory and gastrointestinal illnesses, due to enrichment with monoterpenes and sesquiterpenes. Other secondary metabolites found in Thymus species, namely phenolic acids, flavonoids, flavone glucuronides, show numerous additional health-promoting effects (antioxidant, antidiabetic, anticancer). In this work, the antihepatoma activity of methanol extracts from in vitro shoot cultures of T. pannonicus citral chemotype on human HEPG2 hepatoma cells was evaluated. Two methanol extracts were investigated: E1 – derived from shoots grown on a medium without plant growth regulators and E2 – derived from shoots grown in the presence of the plant hormone indole-3-acetic acid (1mg/ml). Quantitative spectrophotometric analysis revealed that total phenolic and flavonoid contents were higher in E2 (540.4mg/g and 9.84mg/g, respectively) than in E1 (265.4mg/g and 6.52mg/g, respectively). Further analysis using HPLC identified rosmarinic acid (RA) as the dominant phenolic acid in both extracts, with E2 containing a slightly higher amount of RA than E1. The extracts reduced growth of HEPG2 cells in a dose- and time-dependent manner with moderate IC50 values (IC50(E1)=81.1µg/ml; IC50(E2)=77.8µg/ml, MTT, 96h), as shown by cell viability tests. Similarly, RA, used as a control, suppressed the growth of HEPG2 cells with an IC50=30.8µM (MTT, 96h). Flow cytometry of propidium iodide-stained cells showed that T. pannonicus extracts and RA arrested HEPG2 cells in the G2/M phase of the cell cycle, while fluorimetric measurement of cells loaded with dihydrorhodamine revealed mild elevation of intracellular ROS content induced by both extracts after 48h of treatment. Considering high content of RA in both extracts and similar antiproliferative effect of RA, E1 and E2 on HEPG2 cells, it could be proposed that RA is the active compound responsible for moderate antihepatoma activity of the investigated T. pannonicus extracts",
publisher = "Sarajevo: Institute for Genetic Engineering and Biotechnology, University of Sarajevo",
journal = "Book of abstracts: 2nd B&H Symposium of Laboratory Geneticists and Molecular Biologists (with International Participation); 2024 May 10-11; Banja Luka, Bosnia and Herzegovina",
title = "Antihepatoma activity of methanol extracts from Thymus pannonicus in vitro shoot cultures",
pages = "27",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6712"
}

Tovilović-Kovačević, G., Ignjatović, Đ., Krstić-Milošević, D., Tomić, M.,& Ćosić, T.. (2024). Antihepatoma activity of methanol extracts from Thymus pannonicus in vitro shoot cultures. in Book of abstracts: 2nd B&H Symposium of Laboratory Geneticists and Molecular Biologists (with International Participation); 2024 May 10-11; Banja Luka, Bosnia and Herzegovina
Sarajevo: Institute for Genetic Engineering and Biotechnology, University of Sarajevo., 27.
https://hdl.handle.net/21.15107/rcub_ibiss_6712

Tovilović-Kovačević G, Ignjatović Đ, Krstić-Milošević D, Tomić M, Ćosić T. Antihepatoma activity of methanol extracts from Thymus pannonicus in vitro shoot cultures. in Book of abstracts: 2nd B&H Symposium of Laboratory Geneticists and Molecular Biologists (with International Participation); 2024 May 10-11; Banja Luka, Bosnia and Herzegovina. 2024;:27.
https://hdl.handle.net/21.15107/rcub_ibiss_6712 .

Tovilović-Kovačević, Gordana, Ignjatović, Đurđica, Krstić-Milošević, Dijana, Tomić, Mirko, Ćosić, Tatjana, "Antihepatoma activity of methanol extracts from Thymus pannonicus in vitro shoot cultures" in Book of abstracts: 2nd B&H Symposium of Laboratory Geneticists and Molecular Biologists (with International Participation); 2024 May 10-11; Banja Luka, Bosnia and Herzegovina (2024):27,
https://hdl.handle.net/21.15107/rcub_ibiss_6712 .

TY  - CONF
AU  - Ignjatović, Đurđica
AU  - Nestorović, Nataša
AU  - Tomić, Mirko
AU  - Ristić, Nataša
AU  - Veličković, Nataša
AU  - Perović, Milka
AU  - Tovilović-Kovačević, Gordana
AU  - Manojlović-Stojanoski, Milica
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6711
AB  - During prenatal development, glucocorticoids control fetal growth and maturation of fetal tissues. Synthetic glucocorticoids are frequently administered to pregnant women at risk for preterm delivery. Despite their beneficial effects on lung maturation and fetal survival, their impact on the developing brain is less clear. Among postnatal factors that might have a profound effect on both the cognitive capacity and behavior of the offspring, high fructose consumption is particularly concerning in the young population. The present study aimed to investigate the effects of prenatal dexamethasone exposure additionally challenged with postnatal fructose overconsumption on anxiety and recognition memory performance in male Wistar rat offspring. Pregnant female rats were treated with three doses of dexamethasone (0.5 mg/kg/b.m.) during late gestation (16th-18th gestational day), corresponding to clinical human exposure. Male offspring were supplemented with a moderate dose of fructose, similar to those found in sugar-sweetened beverages (10% solution), from weaning till adulthood. Recognition memory and anxiety-like behavior were assessed using a novel object recognition test, and elevated plus maze, respectively. Hippocampal synaptic plasticity was estimated by the levels of GAP-43, synaptophysin, postsynaptic density protein 95 (PSD-95), and its activating phosphorylation (pPSD-95-Ser295). Prenatal dexamethasone treatment induced an anxiolytic-like effect, stimulation of exploratory behavior, and the improvement of recognition memory associated with an increase in GAP-43 protein level in the hippocampus. Fructose overconsumption after weaning did not modify the effects of prenatal glucocorticoid exposure in the applied experimental model, suggesting that fetal programming had a prevailing influence. According to our results, prenatal dexamethasone treatment may induce changes in reactions to novel situations in male Wistar rats which might represent advantageous fetal developmental adaptation to a new environment, while increased exploratory behavior, reduced anxiety, and improved ability to recognize novel objects could improve survival in an adverse postnatal environment.
PB  - Sarajevo: Institute for Genetic Engineering and Biotechnology, University of Sarajevo
C3  - Book of abstracts: 2nd B&H Symposium of Laboratory Geneticists and Molecular Biologists (with International Participation); 2024 May 10-11; Banja Luka, Bosnia and Herzegovina
T1  - Effects of prenatal dexamethasone treatment and moderate post-weaning fructose consumption on recognition memory in adult male Wistar rat offspring
SP  - 34
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6711
ER  - 

@conference{
author = "Ignjatović, Đurđica and Nestorović, Nataša and Tomić, Mirko and Ristić, Nataša and Veličković, Nataša and Perović, Milka and Tovilović-Kovačević, Gordana and Manojlović-Stojanoski, Milica",
year = "2024",
abstract = "During prenatal development, glucocorticoids control fetal growth and maturation of fetal tissues. Synthetic glucocorticoids are frequently administered to pregnant women at risk for preterm delivery. Despite their beneficial effects on lung maturation and fetal survival, their impact on the developing brain is less clear. Among postnatal factors that might have a profound effect on both the cognitive capacity and behavior of the offspring, high fructose consumption is particularly concerning in the young population. The present study aimed to investigate the effects of prenatal dexamethasone exposure additionally challenged with postnatal fructose overconsumption on anxiety and recognition memory performance in male Wistar rat offspring. Pregnant female rats were treated with three doses of dexamethasone (0.5 mg/kg/b.m.) during late gestation (16th-18th gestational day), corresponding to clinical human exposure. Male offspring were supplemented with a moderate dose of fructose, similar to those found in sugar-sweetened beverages (10% solution), from weaning till adulthood. Recognition memory and anxiety-like behavior were assessed using a novel object recognition test, and elevated plus maze, respectively. Hippocampal synaptic plasticity was estimated by the levels of GAP-43, synaptophysin, postsynaptic density protein 95 (PSD-95), and its activating phosphorylation (pPSD-95-Ser295). Prenatal dexamethasone treatment induced an anxiolytic-like effect, stimulation of exploratory behavior, and the improvement of recognition memory associated with an increase in GAP-43 protein level in the hippocampus. Fructose overconsumption after weaning did not modify the effects of prenatal glucocorticoid exposure in the applied experimental model, suggesting that fetal programming had a prevailing influence. According to our results, prenatal dexamethasone treatment may induce changes in reactions to novel situations in male Wistar rats which might represent advantageous fetal developmental adaptation to a new environment, while increased exploratory behavior, reduced anxiety, and improved ability to recognize novel objects could improve survival in an adverse postnatal environment.",
publisher = "Sarajevo: Institute for Genetic Engineering and Biotechnology, University of Sarajevo",
journal = "Book of abstracts: 2nd B&H Symposium of Laboratory Geneticists and Molecular Biologists (with International Participation); 2024 May 10-11; Banja Luka, Bosnia and Herzegovina",
title = "Effects of prenatal dexamethasone treatment and moderate post-weaning fructose consumption on recognition memory in adult male Wistar rat offspring",
pages = "34",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6711"
}

Ignjatović, Đ., Nestorović, N., Tomić, M., Ristić, N., Veličković, N., Perović, M., Tovilović-Kovačević, G.,& Manojlović-Stojanoski, M.. (2024). Effects of prenatal dexamethasone treatment and moderate post-weaning fructose consumption on recognition memory in adult male Wistar rat offspring. in Book of abstracts: 2nd B&H Symposium of Laboratory Geneticists and Molecular Biologists (with International Participation); 2024 May 10-11; Banja Luka, Bosnia and Herzegovina
Sarajevo: Institute for Genetic Engineering and Biotechnology, University of Sarajevo., 34.
https://hdl.handle.net/21.15107/rcub_ibiss_6711

Ignjatović Đ, Nestorović N, Tomić M, Ristić N, Veličković N, Perović M, Tovilović-Kovačević G, Manojlović-Stojanoski M. Effects of prenatal dexamethasone treatment and moderate post-weaning fructose consumption on recognition memory in adult male Wistar rat offspring. in Book of abstracts: 2nd B&H Symposium of Laboratory Geneticists and Molecular Biologists (with International Participation); 2024 May 10-11; Banja Luka, Bosnia and Herzegovina. 2024;:34.
https://hdl.handle.net/21.15107/rcub_ibiss_6711 .

Ignjatović, Đurđica, Nestorović, Nataša, Tomić, Mirko, Ristić, Nataša, Veličković, Nataša, Perović, Milka, Tovilović-Kovačević, Gordana, Manojlović-Stojanoski, Milica, "Effects of prenatal dexamethasone treatment and moderate post-weaning fructose consumption on recognition memory in adult male Wistar rat offspring" in Book of abstracts: 2nd B&H Symposium of Laboratory Geneticists and Molecular Biologists (with International Participation); 2024 May 10-11; Banja Luka, Bosnia and Herzegovina (2024):34,
https://hdl.handle.net/21.15107/rcub_ibiss_6711 .

TY  - CONF
AU  - Smailagić, Dijana
AU  - Dragišić Maksimović, Jelena
AU  - Marin, Marija
AU  - Banjac, Nevena
AU  - Ninković, Slavica
AU  - Stupar, Sofija
AU  - Stanišić, Mariana
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6710
AB  - Phloretin is a phenolic compound from the apple that belongs to the group of dihydrochalcones. The genus Malus is a uniquely rich source of dihydrochalcones, accounting for about 97% of the total phenolic compounds in apple leaves and 10-20% of the dry weight of the leaf tissue. Due to its typical dihydrochalcone structure, phloretin is a highly reactive molecule that interacts efficiently with many biological macromolecules. Its reactivity leads to remarkable antioxidant and medicinal properties that have been extensively studied in mammals and humans. However, knowledge about the effects of phloretin on plant organisms is rather scarce. The phytotoxicity of phloretin in plants was first documented by our research group when phloretin was recognised as a potent allelochemical of the apple tree with deleterious effects in plant species outside the genus Malus. Research on the model plant Arabidopsis [Arabidopsis thaliana (L.) Heynh] named the disruption of auxin homeostasis in the roots as the main mechanism of the phytotoxic effect of phloretin. The present study complements previous research with new insights into the ultrastructural changes in leaf mesophyll cells that underlie the growth arrest and decay of the aerial parts of Arabidopsis seedlings exposed to phloretin. The effects of phloretin are closely linked to the ultrastructural damage in the leaf mesophyll cells and range from mild effects with short-term treatment to severe effects with long-term treatment. Transmission electron microscopy revealed changes in the morphology of the cell wall, changes in thylakoid organisation, swelling and displacement of chloroplasts after short-term treatment. A significant decrease in the number of starch granules and chlorophyll content in the leaves reflected impaired chloroplast function leading to depletion of starch reserves, starvation of cells and recruitment of micro- and macroautophagic processes for self-maintenance leading to programmed cell death after long-term treatment with phloretin. These results confirm that the sites of action of phloretin in plant and animal cells do not coincide, confirming the safety of its potential application as a new bioherbicidal agent for humans and animals.
PB  - Turkey. Gebze,Turkey: Technical University
C3  - Book of Abstracts: 11th International Congress on Microscopy & Spectroscopy (INTERM 2024); 2024 Apr 18-24; Oludeniz, Turkey
T1  - How Does The Health-Promoting Apple Phenolic Phloretin Affect The Cell Ultrastructure in Arabidopsis Shoots?
SP  - ID-739
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6710
ER  - 

@conference{
author = "Smailagić, Dijana and Dragišić Maksimović, Jelena and Marin, Marija and Banjac, Nevena and Ninković, Slavica and Stupar, Sofija and Stanišić, Mariana",
year = "2024",
abstract = "Phloretin is a phenolic compound from the apple that belongs to the group of dihydrochalcones. The genus Malus is a uniquely rich source of dihydrochalcones, accounting for about 97% of the total phenolic compounds in apple leaves and 10-20% of the dry weight of the leaf tissue. Due to its typical dihydrochalcone structure, phloretin is a highly reactive molecule that interacts efficiently with many biological macromolecules. Its reactivity leads to remarkable antioxidant and medicinal properties that have been extensively studied in mammals and humans. However, knowledge about the effects of phloretin on plant organisms is rather scarce. The phytotoxicity of phloretin in plants was first documented by our research group when phloretin was recognised as a potent allelochemical of the apple tree with deleterious effects in plant species outside the genus Malus. Research on the model plant Arabidopsis [Arabidopsis thaliana (L.) Heynh] named the disruption of auxin homeostasis in the roots as the main mechanism of the phytotoxic effect of phloretin. The present study complements previous research with new insights into the ultrastructural changes in leaf mesophyll cells that underlie the growth arrest and decay of the aerial parts of Arabidopsis seedlings exposed to phloretin. The effects of phloretin are closely linked to the ultrastructural damage in the leaf mesophyll cells and range from mild effects with short-term treatment to severe effects with long-term treatment. Transmission electron microscopy revealed changes in the morphology of the cell wall, changes in thylakoid organisation, swelling and displacement of chloroplasts after short-term treatment. A significant decrease in the number of starch granules and chlorophyll content in the leaves reflected impaired chloroplast function leading to depletion of starch reserves, starvation of cells and recruitment of micro- and macroautophagic processes for self-maintenance leading to programmed cell death after long-term treatment with phloretin. These results confirm that the sites of action of phloretin in plant and animal cells do not coincide, confirming the safety of its potential application as a new bioherbicidal agent for humans and animals.",
publisher = "Turkey. Gebze,Turkey: Technical University",
journal = "Book of Abstracts: 11th International Congress on Microscopy & Spectroscopy (INTERM 2024); 2024 Apr 18-24; Oludeniz, Turkey",
title = "How Does The Health-Promoting Apple Phenolic Phloretin Affect The Cell Ultrastructure in Arabidopsis Shoots?",
pages = "ID-739",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6710"
}

Smailagić, D., Dragišić Maksimović, J., Marin, M., Banjac, N., Ninković, S., Stupar, S.,& Stanišić, M.. (2024). How Does The Health-Promoting Apple Phenolic Phloretin Affect The Cell Ultrastructure in Arabidopsis Shoots?. in Book of Abstracts: 11th International Congress on Microscopy & Spectroscopy (INTERM 2024); 2024 Apr 18-24; Oludeniz, Turkey
Turkey. Gebze,Turkey: Technical University., ID-739.
https://hdl.handle.net/21.15107/rcub_ibiss_6710

Smailagić D, Dragišić Maksimović J, Marin M, Banjac N, Ninković S, Stupar S, Stanišić M. How Does The Health-Promoting Apple Phenolic Phloretin Affect The Cell Ultrastructure in Arabidopsis Shoots?. in Book of Abstracts: 11th International Congress on Microscopy & Spectroscopy (INTERM 2024); 2024 Apr 18-24; Oludeniz, Turkey. 2024;:ID-739.
https://hdl.handle.net/21.15107/rcub_ibiss_6710 .

Smailagić, Dijana, Dragišić Maksimović, Jelena, Marin, Marija, Banjac, Nevena, Ninković, Slavica, Stupar, Sofija, Stanišić, Mariana, "How Does The Health-Promoting Apple Phenolic Phloretin Affect The Cell Ultrastructure in Arabidopsis Shoots?" in Book of Abstracts: 11th International Congress on Microscopy & Spectroscopy (INTERM 2024); 2024 Apr 18-24; Oludeniz, Turkey (2024):ID-739,
https://hdl.handle.net/21.15107/rcub_ibiss_6710 .

TY  - CONF
AU  - Milićević, Ana
AU  - Popović, Slađana
AU  - Bjelica, Vukašin
AU  - Vasiljević, Božica
AU  - Krizmanić, Jelena
AU  - Jakovljević, Olga
PY  - 2024
UR  - https://15edm2024.mk/program/
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6709
AB  - Due to their high water temperature and special water chemistry, thermal springs offer unique ecological conditions for microbial communities. They are considered to be very interesting and important habitats for the study of algal flora, including diatom biodiversity. However, their occurrence in Serbian thermal springs is scarcely investigated. The aim of this study was to assess the diversity of the diatom community in five
thermal springs in central Serbia (Vrujci spa, Bukovička spa, Omoljica, Ovčanska spa and Poljane). Samples were collected from natural thermal springs except from Bukovička spa, where they were taken
from the tap. In Vrujci spa, samples were collected both from the tap and from the natural spring. During the autumn and winter in 2023, a total of 11 biofilm samples were collected with a sterile scalpel. The
samples were treated with HCl and KMnO4 in the laboratory, afterwards permanent diatom slides were made. The Carl Zeiss AxioImager.M1 light microscope with DIC optics, AxioCam MRc5 and AxioVision4.9
software were used for microscopic analysis. During survey, in total 82 diatom taxa belonging to 33 genera were identified. The most taxon-rich genera were Nitzschia and Navicula with 13 and 10 taxa, respectively. Our preliminary results show that the samples from the Vrujci spa have
the highest diatom diversity (62 taxa) and from Bukovička the lowest (5 taxa). In the Vrujci spa, 40 taxa were identified in the natural thermal spring, while 22 were recorded at the tap. The highest temperature
(25.1°C) was measured at this site compared to the other thermal springs. The dominant taxa were Staurosira venter and Achnanthidium straubianum, which distinguishes this natural thermal spring from the others. At the tap in Vrujci, the dominance of Navicula veneta, Nitzschia palea and Achnanthidium microcephalum showed that the communities at natural and artificial sites differ greatly. In Bukovička spa, the most abundant taxa included N. palea and N. veneta. In the Omoljica thermal spring, 19 taxa were identified with dominance of N. palea, followed by Gomphonema parvulum and N. veneta with slightly lower abundances. In Poljane, N. palea and A. microcephalum were dominant from 11 recorded taxa. In Ovčanska spa, Nitzschia thermaloides was found to be predominant. In addition, the other 18 taxa found at this thermal spring occur with very low abundances. Additional taxonomic insights are required to more precisely define the diatom communities and the ecology of the taxa inhabiting thermal springs. Besides, occurrence of rare species can be expected in this types of extreme habitats.
PB  - Skopje: Faculty of Natural Sciences and Mathematics
C3  - Book of abstracts: 15th European Diatom Meeting; 2024 May 7-9; Ohrid, North Macedonia
T1  - Diatom diversity of five thermal springs in Serbia
SP  - 154
EP  - 155
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6709
ER  - 

@conference{
author = "Milićević, Ana and Popović, Slađana and Bjelica, Vukašin and Vasiljević, Božica and Krizmanić, Jelena and Jakovljević, Olga",
year = "2024",
abstract = "Due to their high water temperature and special water chemistry, thermal springs offer unique ecological conditions for microbial communities. They are considered to be very interesting and important habitats for the study of algal flora, including diatom biodiversity. However, their occurrence in Serbian thermal springs is scarcely investigated. The aim of this study was to assess the diversity of the diatom community in five
thermal springs in central Serbia (Vrujci spa, Bukovička spa, Omoljica, Ovčanska spa and Poljane). Samples were collected from natural thermal springs except from Bukovička spa, where they were taken
from the tap. In Vrujci spa, samples were collected both from the tap and from the natural spring. During the autumn and winter in 2023, a total of 11 biofilm samples were collected with a sterile scalpel. The
samples were treated with HCl and KMnO4 in the laboratory, afterwards permanent diatom slides were made. The Carl Zeiss AxioImager.M1 light microscope with DIC optics, AxioCam MRc5 and AxioVision4.9
software were used for microscopic analysis. During survey, in total 82 diatom taxa belonging to 33 genera were identified. The most taxon-rich genera were Nitzschia and Navicula with 13 and 10 taxa, respectively. Our preliminary results show that the samples from the Vrujci spa have
the highest diatom diversity (62 taxa) and from Bukovička the lowest (5 taxa). In the Vrujci spa, 40 taxa were identified in the natural thermal spring, while 22 were recorded at the tap. The highest temperature
(25.1°C) was measured at this site compared to the other thermal springs. The dominant taxa were Staurosira venter and Achnanthidium straubianum, which distinguishes this natural thermal spring from the others. At the tap in Vrujci, the dominance of Navicula veneta, Nitzschia palea and Achnanthidium microcephalum showed that the communities at natural and artificial sites differ greatly. In Bukovička spa, the most abundant taxa included N. palea and N. veneta. In the Omoljica thermal spring, 19 taxa were identified with dominance of N. palea, followed by Gomphonema parvulum and N. veneta with slightly lower abundances. In Poljane, N. palea and A. microcephalum were dominant from 11 recorded taxa. In Ovčanska spa, Nitzschia thermaloides was found to be predominant. In addition, the other 18 taxa found at this thermal spring occur with very low abundances. Additional taxonomic insights are required to more precisely define the diatom communities and the ecology of the taxa inhabiting thermal springs. Besides, occurrence of rare species can be expected in this types of extreme habitats.",
publisher = "Skopje: Faculty of Natural Sciences and Mathematics",
journal = "Book of abstracts: 15th European Diatom Meeting; 2024 May 7-9; Ohrid, North Macedonia",
title = "Diatom diversity of five thermal springs in Serbia",
pages = "154-155",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6709"
}

Milićević, A., Popović, S., Bjelica, V., Vasiljević, B., Krizmanić, J.,& Jakovljević, O.. (2024). Diatom diversity of five thermal springs in Serbia. in Book of abstracts: 15th European Diatom Meeting; 2024 May 7-9; Ohrid, North Macedonia
Skopje: Faculty of Natural Sciences and Mathematics., 154-155.
https://hdl.handle.net/21.15107/rcub_ibiss_6709

Milićević A, Popović S, Bjelica V, Vasiljević B, Krizmanić J, Jakovljević O. Diatom diversity of five thermal springs in Serbia. in Book of abstracts: 15th European Diatom Meeting; 2024 May 7-9; Ohrid, North Macedonia. 2024;:154-155.
https://hdl.handle.net/21.15107/rcub_ibiss_6709 .

Milićević, Ana, Popović, Slađana, Bjelica, Vukašin, Vasiljević, Božica, Krizmanić, Jelena, Jakovljević, Olga, "Diatom diversity of five thermal springs in Serbia" in Book of abstracts: 15th European Diatom Meeting; 2024 May 7-9; Ohrid, North Macedonia (2024):154-155,
https://hdl.handle.net/21.15107/rcub_ibiss_6709 .

TY  - JOUR
AU  - Bajić, Branka
AU  - Budinski, Ivana
AU  - Rajičić, Marija
AU  - Miljević, Milan
AU  - Rončević, Aleksa
AU  - Blagojević, Jelena
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6707
AB  - Schreiber’s bent-winged bat Miniopterus schreibersii (Chiroptera) is a widespread, cave-dwelling, regionally migrating species whose genetic diversity was studied throughout its distribution area using mitochondrial and nuclear markers. Previous studies revealed little to no structuring of populations and established Anatolia to be a single refugium during the last glacial maximum. The Balkans were well covered in these studies but usually lacked samples from Serbia. We sequenced the mitochondrial hypervariable region 1 (HV1) gene in M. schreibersii collected at seven sites in Serbia to assess their genetic relatedness to other European and Asia Minor populations and check whether the Balkans exhibit a higher genetic diversity than Western Europe due to its closeness to Anatolia. We recorded nine haplotypes from Serbia, six of which had not been previously reported, with a haplotype diversity of 0.585. The remaining three were shared with individuals from Portugal, Greece, and Turkey. A single most common haplotype was present throughout the species distribution range, pointing to a well-connected population and as indicated by the shape of the haplotype network, a common origin, and a sudden population expansion. Results complement existing data on M. schreibersii having a non-structured population, adding valuable new data from the Balkans supporting the previous hypothesis about its Anatolian origin.
PB  - Belgrade: Serbian Biological Society
T2  - Archives of Biological Sciences
T1  - A first look at mitochondrial genetic diversity in Miniopterus schreibersii in Serbia
IS  - 1
VL  - 76
DO  - 10.2298/ABS240208006B
SP  - 103
EP  - 110
ER  - 

@article{
author = "Bajić, Branka and Budinski, Ivana and Rajičić, Marija and Miljević, Milan and Rončević, Aleksa and Blagojević, Jelena",
year = "2024",
abstract = "Schreiber’s bent-winged bat Miniopterus schreibersii (Chiroptera) is a widespread, cave-dwelling, regionally migrating species whose genetic diversity was studied throughout its distribution area using mitochondrial and nuclear markers. Previous studies revealed little to no structuring of populations and established Anatolia to be a single refugium during the last glacial maximum. The Balkans were well covered in these studies but usually lacked samples from Serbia. We sequenced the mitochondrial hypervariable region 1 (HV1) gene in M. schreibersii collected at seven sites in Serbia to assess their genetic relatedness to other European and Asia Minor populations and check whether the Balkans exhibit a higher genetic diversity than Western Europe due to its closeness to Anatolia. We recorded nine haplotypes from Serbia, six of which had not been previously reported, with a haplotype diversity of 0.585. The remaining three were shared with individuals from Portugal, Greece, and Turkey. A single most common haplotype was present throughout the species distribution range, pointing to a well-connected population and as indicated by the shape of the haplotype network, a common origin, and a sudden population expansion. Results complement existing data on M. schreibersii having a non-structured population, adding valuable new data from the Balkans supporting the previous hypothesis about its Anatolian origin.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Archives of Biological Sciences",
title = "A first look at mitochondrial genetic diversity in Miniopterus schreibersii in Serbia",
number = "1",
volume = "76",
doi = "10.2298/ABS240208006B",
pages = "103-110"
}

Bajić, B., Budinski, I., Rajičić, M., Miljević, M., Rončević, A.,& Blagojević, J.. (2024). A first look at mitochondrial genetic diversity in Miniopterus schreibersii in Serbia. in Archives of Biological Sciences
Belgrade: Serbian Biological Society., 76(1), 103-110.
https://doi.org/10.2298/ABS240208006B

Bajić B, Budinski I, Rajičić M, Miljević M, Rončević A, Blagojević J. A first look at mitochondrial genetic diversity in Miniopterus schreibersii in Serbia. in Archives of Biological Sciences. 2024;76(1):103-110.
doi:10.2298/ABS240208006B .

Bajić, Branka, Budinski, Ivana, Rajičić, Marija, Miljević, Milan, Rončević, Aleksa, Blagojević, Jelena, "A first look at mitochondrial genetic diversity in Miniopterus schreibersii in Serbia" in Archives of Biological Sciences, 76, no. 1 (2024):103-110,
https://doi.org/10.2298/ABS240208006B . .

TY  - JOUR
AU  - Rašić-Marković, Aleksandra
AU  - Đurić, Emilija
AU  - Skrijelj, Daniel
AU  - Bjekić-Macut, Jelica
AU  - Ignjatović, Đurđica
AU  - Šutulović, Nikola
AU  - Hrnčić, Dragan
AU  - Mladenović, Dušan
AU  - Marković, Aleksandra
AU  - Radenković, Saša
AU  - Radić, Lena
AU  - Radunović, Nebojša
AU  - Stanojlović, Olivera
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6708
AB  - Neuroactive steroids are a type of steroid hormones produced within the nervous system or in peripheral glands and then transported to the brain to exert their neuromodulatory effects. Neuroactive steroids have pleiotropic effects, that include promoting myelination, neuroplasticity, and brain development. They also regulate important physiological functions, such as metabolism, feeding, reproduction, and stress response. The homoeostatic processes of metabolism and reproduction are closely linked and mutually dependent. Reproductive events, such as pregnancy, bring about significant changes in metabolism, and metabolic status may affect reproductive function in mammals. In females, the regulation of reproduction and energy balance is controlled by the fluctuations of oestradiol and progesterone throughout the menstrual cycle. Neurosteroids play a key role in the neuroendocrine control of reproduction. The synthesis of neuroestradiol and neuroprogesterone within the brain is a crucial process that facilitates the release of GnRH and LH, which in turn, regulate the transition from oestrogen-negative to oestrogen-positive feedback. In addition to their function in the reproductive system, oestrogen has a key role in the regulation of energy homoeostasis by acting at central and peripheral levels. The oestrogenic effects on body weight homoeostasis are primarily mediated by oestrogen receptors-α (ERα), which are abundantly expressed in multiple brain regions that are implicated in the regulation of food intake, basal metabolism, thermogenesis, and brown tissue distribution. The tight interplay between energy balance and reproductive physiology is facilitated by shared regulatory pathways, namely POMC, NPY and kisspeptin neurons, which are targets of oestrogen regulation and likely participate in different aspects of the joint control of energy balance and reproductive function. The aim of this review is to present a summary of the progress made in uncovering shared regulatory pathways that facilitate the tight coupling between energy balance and reproductive physiology, as well as their reciprocal interactions and the modulation induced by neurosteroids.
PB  - Springer Nature
T2  - Endocrine
T1  - Neuroactive steroids in the neuroendocrine control of food intake, metabolism, and reproduction
DO  - 10.1007/s12020-024-03755-x
ER  - 

@article{
author = "Rašić-Marković, Aleksandra and Đurić, Emilija and Skrijelj, Daniel and Bjekić-Macut, Jelica and Ignjatović, Đurđica and Šutulović, Nikola and Hrnčić, Dragan and Mladenović, Dušan and Marković, Aleksandra and Radenković, Saša and Radić, Lena and Radunović, Nebojša and Stanojlović, Olivera",
year = "2024",
abstract = "Neuroactive steroids are a type of steroid hormones produced within the nervous system or in peripheral glands and then transported to the brain to exert their neuromodulatory effects. Neuroactive steroids have pleiotropic effects, that include promoting myelination, neuroplasticity, and brain development. They also regulate important physiological functions, such as metabolism, feeding, reproduction, and stress response. The homoeostatic processes of metabolism and reproduction are closely linked and mutually dependent. Reproductive events, such as pregnancy, bring about significant changes in metabolism, and metabolic status may affect reproductive function in mammals. In females, the regulation of reproduction and energy balance is controlled by the fluctuations of oestradiol and progesterone throughout the menstrual cycle. Neurosteroids play a key role in the neuroendocrine control of reproduction. The synthesis of neuroestradiol and neuroprogesterone within the brain is a crucial process that facilitates the release of GnRH and LH, which in turn, regulate the transition from oestrogen-negative to oestrogen-positive feedback. In addition to their function in the reproductive system, oestrogen has a key role in the regulation of energy homoeostasis by acting at central and peripheral levels. The oestrogenic effects on body weight homoeostasis are primarily mediated by oestrogen receptors-α (ERα), which are abundantly expressed in multiple brain regions that are implicated in the regulation of food intake, basal metabolism, thermogenesis, and brown tissue distribution. The tight interplay between energy balance and reproductive physiology is facilitated by shared regulatory pathways, namely POMC, NPY and kisspeptin neurons, which are targets of oestrogen regulation and likely participate in different aspects of the joint control of energy balance and reproductive function. The aim of this review is to present a summary of the progress made in uncovering shared regulatory pathways that facilitate the tight coupling between energy balance and reproductive physiology, as well as their reciprocal interactions and the modulation induced by neurosteroids.",
publisher = "Springer Nature",
journal = "Endocrine",
title = "Neuroactive steroids in the neuroendocrine control of food intake, metabolism, and reproduction",
doi = "10.1007/s12020-024-03755-x"
}

Rašić-Marković, A., Đurić, E., Skrijelj, D., Bjekić-Macut, J., Ignjatović, Đ., Šutulović, N., Hrnčić, D., Mladenović, D., Marković, A., Radenković, S., Radić, L., Radunović, N.,& Stanojlović, O.. (2024). Neuroactive steroids in the neuroendocrine control of food intake, metabolism, and reproduction. in Endocrine
Springer Nature..
https://doi.org/10.1007/s12020-024-03755-x

Rašić-Marković A, Đurić E, Skrijelj D, Bjekić-Macut J, Ignjatović Đ, Šutulović N, Hrnčić D, Mladenović D, Marković A, Radenković S, Radić L, Radunović N, Stanojlović O. Neuroactive steroids in the neuroendocrine control of food intake, metabolism, and reproduction. in Endocrine. 2024;.
doi:10.1007/s12020-024-03755-x .

Rašić-Marković, Aleksandra, Đurić, Emilija, Skrijelj, Daniel, Bjekić-Macut, Jelica, Ignjatović, Đurđica, Šutulović, Nikola, Hrnčić, Dragan, Mladenović, Dušan, Marković, Aleksandra, Radenković, Saša, Radić, Lena, Radunović, Nebojša, Stanojlović, Olivera, "Neuroactive steroids in the neuroendocrine control of food intake, metabolism, and reproduction" in Endocrine (2024),
https://doi.org/10.1007/s12020-024-03755-x . .