Enzymatic biomarkers as indicators of dietary cadmium in gypsy moth caterpillars
2013
Authors:
Vlahović, MilenaPerić Mataruga, Vesna
Mrdaković, Marija
Matić, Dragana
Lazarević, Jelica
Nenadović, Vera
Ilijin, Larisa
Document Type:
Article (Published version)
,
2012, Springer-Verlag Berlin Heidelberg
Metadata
Show full item recordAbstract:
Heavy metals damage the structure, chemistry, and function of cells, including enzyme systems inside them. Variation in the profile of biochemical biomarkers in prevalent species should be used for assessing environmental contamination. The present study pays attention to the phosphatases present in the midgut of gypsy moth fourth instar caterpillars, which had been exposed to short- and long-term cadmium intake at 10 and 30 μg Cd/g dry food. Chronic cadmium ingestion significantly inhibited the activity of all examined phosphatases, while only the activity of lysosomal phosphatase was acutely decreased. Total acid phosphatase activity recovered from both long-term cadmium treatments within 3 days. The low index of phenotypic plasticity was connected to high variability of plasticity. Dependence of phosphatase isoforms on genotype and duration of cadmium treatment was determined. We concluded that, with further investigations, profiling of total acid phosphatase activity, as well as the lysosomal fraction can be used as a biomarker for acute sublethal metal toxicity.
Keywords:
Gypsy moth; Cadmium; Phosphatases; Acute and chronic toxicity; Enzyme variability; BiomarkersSource:
Environmental Science and Pollution Research, 2013, 20, 5, 3447-3455Funding / projects:
- The effects of magnetic fields and other environmental stressors on the physiological responses and behavior of different species (RS-MESTD-Basic Research (BR or ON)-173027)
DOI: 10.1007/s11356-012-1292-3
ISSN: 0944-1344
PubMed: 23143823