Приказ основних података о документу
Hyperbaric oxygenation alters temporal expression pattern of superoxide dismutase 2 after cortical stab injury in rats
dc.creator | Parabučki, Ana B. | |
dc.creator | Božić, Iva D. | |
dc.creator | Bjelobaba, Ivana | |
dc.creator | Lavrnja, Irena | |
dc.creator | Brkić, Predrag D | |
dc.creator | Jovanović, Tomislav S | |
dc.creator | Savić, Danijela | |
dc.creator | Stojiljković, Mirjana B | |
dc.creator | Peković, Sanja | |
dc.date.accessioned | 2017-11-23T11:12:03Z | |
dc.date.available | 2015-11-17T10:26:51Z | |
dc.date.issued | 2012 | sr |
dc.identifier.issn | 0353-9504 | sr |
dc.identifier.other | Rad_konverzija_3067 | sr |
dc.identifier.uri | https://radar.ibiss.bg.ac.rs/handle/123456789/1072 | |
dc.description.abstract | Aim To evaluate the effect of hyperbaric oxygen therapy (HBOT) on superoxide dismutase 2 (SOD2) expression pattern after the cortical stab injury (CSI). Methods CSI was performed on 88 male Wistar rats, divided into control, sham, lesioned, and HBO groups. HBOT protocol was the following: pressure applied was 2.5 absolute atmospheres, for 60 minutes, once a day for consecutive 3 or 10 days. The pattern of SOD2 expression and cellular localization was analyzed using real-time polymerase chain reaction, Western blot, and double-label fluorescence immunohistochemistry. Neurons undergoing degeneration were visualized with Fluoro-Jade (R) B. Results CSI induced significant transient increase in SOD2 protein levels at day 3 post injury, which was followed by a reduction toward control levels at post-injury day 10. At the same time points, mRNA levels for SOD2 in the injured cortex were down-regulated. Exposure to HBO for 3 days considerably down-regulated SOD2 protein levels in the injured cortex, while after 10 days of HBOT an up-regulation of SOD2 was observed. HBOT significantly increased mRNA levels for SOD2 at both time points compared to the corresponding L group, but they were still lower than in controls. Double immunofluorescence staining revealed that 3 days after CSI, up-regulation of SOD2 was mostly due to an increased expression in reactive astrocytes surrounding the lesion site. HBOT attenuated SOD2 expression both in neuronal and astroglial cells. Fluoro-Jade (R) B labeling showed that HBOT significantly decreased the number of degenerating neurons in the injured cortex. Conclusion HBOT alters SOD2 protein and mRNA levels after brain injury in a time-dependent manner. | en |
dc.description.sponsorship | Ministry of Education, Science and Technological Development, Republic of Serbia [III41014] | sr |
dc.language.iso | English | sr |
dc.rights | restrictedAccess | |
dc.source | Croatian Medical Journal | sr |
dc.title | Hyperbaric oxygenation alters temporal expression pattern of superoxide dismutase 2 after cortical stab injury in rats | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Јовановић, Томислав С; Бркић, Предраг Д; Савић, Данијела З.; Бјелобаба, Ивана; Лаврња, Ирена Ц.; Парабучки, Aна Б.; Божић, Ива Д.; Пековић, Сања М.; Стојиљковић, Мирјана Б; | |
dc.citation.issue | 6 | sr |
dc.citation.volume | 53 | sr |
dc.citation.spage | 217 | sr |
dc.citation.epage | 597 | sr |
dc.type.version | publishedVersion | en |
dc.citation.rank | M22 | |
dc.identifier.rcub | https://hdl.handle.net/21.15107/rcub_ibiss_1072 |
Документи
Датотеке | Величина | Формат | Преглед |
---|---|---|---|
Уз овај запис нема датотека. |