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Orchidectomy of middle-aged rats decreases liver deiodinase 1 and pituitary deiodinase 2 activity
dc.creator | Šošić-Jurjević, Branka | |
dc.creator | Filipović, Branko | |
dc.creator | Renko, Kostja | |
dc.creator | Ajdžanović, Vladimir | |
dc.creator | Manojlović-Stojanoski, Milica | |
dc.creator | Milošević, Verica | |
dc.creator | Koehrle, Josef | |
dc.date.accessioned | 2017-11-23T11:12:25Z | |
dc.date.available | 2900-01-01 | |
dc.date.issued | 2012 | sr |
dc.identifier.issn | 0022-0795 | sr |
dc.identifier.other | Rad_konverzija_3086 | sr |
dc.identifier.uri | https://radar.ibiss.bg.ac.rs/handle/123456789/1091 | |
dc.description.abstract | Endogenous androgens are involved in regulation of thyroid function and metabolism of thyroid hormones. As serum testosterone level progressively declines with age, this regulation may change. We tested how androgen deprivation, achieved by orchidectomy, affects thyroid homeostasis in middle-aged rats. Fifteen-month-old Wistar rats were orchidectomized (Orx) or sham-operated under ketamine anesthesia (15 mg/kg body weight). Five weeks after the surgery, animals were decapitated. Thyroids were used for histomorphometric and ultrastructural examinations and together with livers and pituitaries for real-time quantitative PCR and deiodinase (DIO) activity measurements. Serum testosterone, TSH, L-thyroxine (T-4), and cholesterol (Chol) levels were determined. As expected, middle-aged control rats had lower (P<0.05) testosterone and T-4 compared with 3-month-old males. In the Orx middle-aged group, we detected diminished serum testosterone (P<0.05), no change in TSH and T-4 levels, and higher Chol level (P<0.05), in comparison with age-matched controls. Histomorphometric analysis of thyroid tissue revealed decreased relative volume densities of follicles and colloid (P<0.05). Relevant gene expressions and DIO1 enzyme activity were not changed in the thyroids of Orx rats. Liver Dio1 gene expression and DIO1 activity were decreased (P<0.05) in comparison with the control values. Pituitary levels of TSH beta, Dio1, and Dio2 mRNAs did not change, while DIO2 activity decreased (P<0.05). In conclusion, orchidectomy of middle-aged rats affected thyroid structure with no effect on serum T-4 and TSH. However, decreased liver DIO1 and pituitary DIO2 enzyme activities indicate compensatory-adaptive changes in local T-3 production. Journal of Endocrinology (2012) 215, 247-256 | en |
dc.description.sponsorship | Ministry of Education and Science of the Republic of Serbia [173009]; Deutsche Forschungsgemeinschaft, Charite Universitatsmedizin Berlin [DFG-GK 1208, TP3]; DAAD fellowship [A/10/05060] | sr |
dc.language.iso | English | sr |
dc.publisher | Society for Endocrinology | |
dc.rights | restrictedAccess | |
dc.source | Journal of Endocrinology | sr |
dc.title | Orchidectomy of middle-aged rats decreases liver deiodinase 1 and pituitary deiodinase 2 activity | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Манојловић-Стојаноски, Милица; Коехрле, Јосеф; Aјджановић, Владимир; Филиповић, Бранко; Шошић-Јурјевић, Бранка Т.; Милошевић, Верица Љ.; Ренко, Костја; | |
dc.rights.holder | © 2012 Society for Endocrinology | |
dc.citation.issue | 2 | sr |
dc.citation.volume | 215 | sr |
dc.identifier.doi | 10.1530/JOE-12-0336 | |
dc.identifier.pmid | 22956722 | |
dc.identifier.scopus | 2-s2.0-84869145272 | |
dc.identifier.wos | 000310221200005 | |
dc.citation.spage | 325 | sr |
dc.citation.epage | 256 | sr |
dc.type.version | publishedVersion | en |
dc.citation.rank | M21 | |
dc.identifier.rcub | https://hdl.handle.net/21.15107/rcub_ibiss_1091 |