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Regulation of ecto-5 '-nucleotidase (CD73) in cultured cortical astrocytes by different inflammatory factors
dc.creator | Brisevac, Dusica | |
dc.creator | Bjelobaba, Ivana | |
dc.creator | Bajić, Aleksandar | |
dc.creator | Clarner, Tim | |
dc.creator | Stojiljković, Mirjana B | |
dc.creator | Beyer, Cordian | |
dc.creator | Anđus, Pavle R | |
dc.creator | Kipp, Markus | |
dc.creator | Nedeljković, Nadezda N | |
dc.date.accessioned | 2017-11-23T11:12:04Z | |
dc.date.available | 2015-11-17T10:26:51Z | |
dc.date.issued | 2012 | sr |
dc.identifier.issn | 0197-0186 | sr |
dc.identifier.other | Rad_konverzija_3093 | sr |
dc.identifier.uri | https://radar.ibiss.bg.ac.rs/handle/123456789/1098 | |
dc.description.abstract | Ecto-5'-nucleotidase (e-5NT) is a cell-surface located, rate-limiting enzyme in the extracellular metabolism of ATP, catalyzing the final step of the conversion of AMP to adenosine. Since this enzyme shifts the balance from pro-inflammatory ATP to anti-inflammatory adenosine, it is considered to be an important regulator of inflammation. Although up-regulation of e-5NT was repeatedly reported in several in vivo models of brain injury, the regulation of its expression and function remains largely unknown. We have studied effects of several pro-inflammatory factors, namely, bacterial endotoxin lipopolysaccharide (LPS), tumor necrosis factor-alpha (TNF-alpha), interferon-gamma (IFN-gamma), glutamate (Glu) and hydrogen peroxide (H2O2) on e-5NT (i) activity, (ii) mRNA expression and (iii) membrane protein abundance in primary cultured cortical astrocytes. We are clearly able to demonstrate a stimulus-specific regulation of the e-5NT pathway. IFN-gamma, LPS, Glu and H2O2 decrease, while TNF-alpha increases e-5NT activity. The analysis of e-5NT gene expression and e-5NT membrane protein levels revealed that tested factors regulate e-5NT at different levels and by employing different mechanisms. In summary, we provide evidence that e-5NT activity is tightly regulated in a stimulus-specific manner. (C) 2012 Elsevier Ltd. All rights reserved. | en |
dc.description.sponsorship | Serbian Ministry of Education and Science [III41014]; Bilateral Project between DAAD/Serbian Ministry of Science and Education; DAAD | sr |
dc.language.iso | English | sr |
dc.rights | restrictedAccess | |
dc.source | Neurochemistry International | sr |
dc.title | Regulation of ecto-5 '-nucleotidase (CD73) in cultured cortical astrocytes by different inflammatory factors | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Брисевац, Дусица; Кипп, Маркус; Aнђус, Павле Р; Беyер, Цордиан; Недељковић, Надезда Н; Стојиљковић, Мирјана Б; Цларнер, Тим; Бајић, Aлександар; Бјелобаба, Ивана; | |
dc.citation.issue | 5 | sr |
dc.citation.volume | 61 | sr |
dc.citation.spage | 406 | sr |
dc.citation.epage | 688 | sr |
dc.type.version | publishedVersion | en |
dc.citation.rank | M22 | |
dc.identifier.rcub | https://hdl.handle.net/21.15107/rcub_ibiss_1098 |
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