Use of Chenopodium murale L. transgenic hairy root in vitro culture system as a new tool for allelopathic assays
2012
Аутори:
Banjac, NevenaDmitrović, Slavica
Đorđević, Mirka
Zdravković-Korać, Snežana
Nikolić, Radomirka R
Raspor, Martin
Ćosić, Tatjana
Maksimović, Vuk M
Živković, Suzana
Krstić Milošević, Dijana
Stanišić, Mariana
Ninković, Slavica
Тип документа:
Чланак у часопису (Објављена верзија)
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© 2012 Elsevier GmbH
Метаподаци
Приказ свих података о документуАпстракт:
We investigated Chenopodium murale transgenic hairy root in vitro culture system as a new tool for allelopathic assays. Transgenic hairy roots were induced by Agrobacterium rhizogenes A4M70GUS from roots, cotyledons, leaves, and internodes of C. murale seedlings. Roots were found to be the best target explants, providing transformation efficiency of up to 11.1%. Established hairy root clones differed in their morphology and growth potential. Molecular characterization of these clones was carried out by PCR, RT-PCR and histochemical GUS analyses. No differences in rol gene expression were observed. Liquid culture system of characterized hairy root clones was maintained for over 2 years. Six hairy root clones were selected for assaying the allelopathic effect of their growth medium against germination and seedling elongation of wheat and lettuce test plants. The inhibitory potential varied depending on the hairy root clone. Some transgenic clones showed significantly higher inhibition compared to wild-type roots. These results revealed that hairy roots as an independent system synthesize some bioactive substances with allelopathic activity and exude them into the growth medium. Concentrations of caffeic, ferulic and p-coumaric acids (0.07-2.85 mu mol/L) identified by HPLC analysis in the growth media were at least 1000 times lower than the inhibitory active concentration (5 mmol/L) of pure grade phenolic acids, suggesting that they have a limited role in the allelopathic phenomena of C murale. The presented hairy root system appears to be a suitable tool for further investigation of the potential and nature of root-mediated allelopathic interference of C. murale. (C) 2012 Elsevier GmbH. All rights reserved.
Извор:
Journal of Plant Physiology, 2012, 169, 12, 323-1211Финансирање / пројекти:
- Биотехнологија ин витро - гајене, лековите и угрожене биљне врсте (RS-173015)
- Физиолошка, хемијска и молекуларна анализа диверзитета одабраних ретких и угрожених биљних врста у циљу еx ситу заштите и продукције биолошки активних једињења (RS-173024)
- Интеракције мембрана са унутарћелијским и апопластичним простором: изучавања биоенергетике и сингализације користећи биофизичке и биохемијске методе (RS-173040)
DOI: 10.1016/j.jplph.2012.04.009
ISSN: 0176-1617