Efekat tiroksina na aktivnost antioksidacionih zaštitnih enzima i količinu glutationa u mozgu pacova različite starosti

Abstract

Effect of thyroxine on antioxidant enzyme activities and GSH content in brain (cortex and rest of the brain) of different maturated rats were studied. Male Mill Hill hybrid hooded rats aged 15, 45 and 75 days were treated with L-thyroxine, T4 (40 μg/100 g body mass), s.c, one dose per day, 14 days (finally aged 30, 60 and 90 days, respectively). Effect of T4 on antioxidant defense (AD) enzyme activities and GSH content in brain differs with respect to age. T4 treatment decrease activities of Mn SOD and CuZn SOD in the rest of the brain of 60 days old rats in comparison to controls and CAT activity in cortex of 30 and 60 day old rats. GSH-Px activity in cortex of 90 days old rats was decreased in T4 treated group in respect to controls, while activity remains unchanged in 30 and 60 day old rats. The activity of GSH-Px in the rest of the brain was unaffected by the treatment in all groups. GSH content in cortex of animals of 30 and 60 days old rats were higher in comparison with corresponding controls, while in 90 days old rats effects were opposite. L- thyroxine treatment significantly decrease GSH content in rest of the brain of 30 days old rats in respect with corresponding controls, while in 60 and 90 days old animals such changes were not detected.

U ovom radu ispitivan je efekat tiroksina na aktivnost antioksidacionih zaštitnih enzima i količinu glutatationa (GSH) u mozgu (moždana kora i ostatak mozga) pacova različite starosti. Mužjaci pacova soja Mili Hill hybrid hooded starosti 15, 45 and 75 dana tretirani su sa L-tiroksinom, T4 (40 μg/100 g telesne mase), s.c, jedna doza dnevno, 14 dana (do finalne starosti 30, 60 i 90 dana). Efekat T4 na aktivnost antioksidacionih zaštitnih enzima i količinu GSH u mozgu razlikuje se sa starošću pacova. Tretman sa T4 smanjuje aktivnost Mn SOD i CuZn SOD u ostatku mozga pacova starih 60 dana u poređenju sa kontrolama i aktivnost CAT u moždanoj kori pacova starih 30 i 60 dana. GSH-Px aktivnost u moždanoj kori pacova starih 90 dana je smanjena kod pacova tretiranih sa T4 u odnosu na kontrole, dok je aktivnost nepromenjena kod pacova starih 30 i 60 dana. Aktivnost GSH-Px u moždanoj kori pacova je nepromenjena u svim ispitivanim grupama. Količina GSH u moždanoj kori pacova starih 30 i 60 dana je povećana u poređenju sa kontrolama, dok je kod pacova starih 90 dana smanjena. Tretman sa L-tiroksinom značajno smanjuje količinu GSH u ostatku mozga pacova starih 30 dana u poređenju sa kontrolama, dok kod pacova starosti 60 i 90 dana promene nisu detektovane.