Plant regeneration from transverse stalk sections of chive plants

Abstract

An efficient system for in vitro regeneration of plants is a prerequisite for plant improvement by using advanced biotechnological techniques. In this work, regeneration of adventitious buds was induced from thin stalk sections (1-2 mm) containing apical shoot meristem of in vitro grown chive (Allium schoenoprasum) plants. Cytokinins 6-furfurylaminopurine (Kin), 6-benzylaminopurine (BA) or thidiazuron (TDZ) at 5 mu M stimulated this process, and Kin was the most efficient among them. Adventitious buds developed further on the same media for an additional 4 weeks and Kin showed the most pronounced effect on shoot and root growth. Thereafter, all acquired shoots were detached and transferred to solid Murashige and Skoog (MS) medium supplemented with 5 mu M Kin. Single shoots developed secondary adventitious shoots and formed bunches. The mean number of adventitious shoots per bunch was the highest in TDZ-induced shoots (33.6), while Kin- and BA-induced shoots formed 11.9 and 11.3 adventitious shoots per bunch, respectively. Kin-induced shoots exhibited the best shoot and root growth. Plants obtained in this study acclimatized readily at high frequency.