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NO modulates the molecular basis of rat interscapular brown adipose tissue thermogenesis
dc.creator | Otašević, Vesna | |
dc.creator | Buzadžić, Biljana J. | |
dc.creator | Korac, Aleksandra B | |
dc.creator | Vasilijević, Ana | |
dc.creator | Janković, Aleksandra | |
dc.creator | Korać, Bato | |
dc.date.accessioned | 2017-11-23T11:10:36Z | |
dc.date.available | 2900-01-01 | |
dc.date.issued | 2010 | sr |
dc.identifier.issn | 1532-0456 | sr |
dc.identifier.other | Rad_konverzija_3351 | sr |
dc.identifier.uri | https://radar.ibiss.bg.ac.rs/handle/123456789/1356 | |
dc.description.abstract | Molecular mechanisms underlying interscapular brown adipose tissue (IBAT) thermogenesis were elucidated. Namely, gene and/or protein expression of uncoupling protein 1 (UCP1), peroxisome proliferator-activated receptor gamma (PPAR gamma), PPAR gamma-coactivator-1 alpha (PGC-1 alpha), vascular endothelial growth factor (VEGF) and proliferating cell nuclear antigen (PCNA) - key molecules that regulate thermogenesis-related processes - mitochondriogenesis, angiogenesis and IBAT hyperplasia, in rats subjected to cold (4 +/- 1 degrees C) for 1, 3, 7, 12, 21 and 45 days were investigated. Particularly, to examine influence of nitric oxide (NO) on IBAT thermogenic-program, cold-exposed animals were treated by L-arginine or N(omega)-nitro-L-arginine-methyl ester (L-NAME). Related to control (22 +/- 1 degrees C), cold induced time-coordinated UCP1, PPAR gamma and PGC-1 alpha transcriptional activation accompanied by PCNA activation and increased VEGF immunolabeling that correlate with endothelial NO synthase (eNOS) transcriptional activation suggesting NO involvement in these thermogenic-factors activation. Observed molecular changes were translated into increased mitochondrialremodeling, angiogenesis, and IBAT hyperplasia. L-Arginine augmented and prolonged cold-induced increase of eNOS, inducible NOS and thermogenic-molecules expression, IBAT nerve supply, vascularity, hyperplasia and mitochondrial-remodeling, while L-NAME had an opposite effects. Results show that NO improves thermogenesis-related mitochondriogenesis, angiogenesis and tissue hyperplasia, positively affecting molecular basis of these processes, suggesting that NO is an essential regulator of IBAT thermogenic-program operating, at genes, proteins and tissue structure levels. (C) 2010 Elsevier Inc. All rights reserved. | en |
dc.description.sponsorship | Ministry of Science and Technological Development of the Republic of Serbia [143050]; [COST FA0602 Action] | sr |
dc.language.iso | English | sr |
dc.publisher | Elsevier | |
dc.rights | restrictedAccess | |
dc.source | Comparative Biochemistry and Physiology C-Toxicology & Pharmacology | sr |
dc.title | NO modulates the molecular basis of rat interscapular brown adipose tissue thermogenesis | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Василијевић, Aна; Корац, Aлександра Б; Кораћ, Бато М.; Јанковић, Aлександра; Бузаджић, Биљана Ј.; Петровић, Весна; | |
dc.rights.holder | © 2010 Elsevier Inc. | |
dc.citation.issue | 2 | sr |
dc.citation.volume | 152 | sr |
dc.identifier.doi | 10.1016/j.cbpc.2010.03.008 | |
dc.identifier.pmid | 20363363 | |
dc.identifier.scopus | 2-s2.0-77952892288 | |
dc.identifier.wos | 000279363400004 | |
dc.citation.spage | 147 | |
dc.citation.epage | 159 | sr |
dc.type.version | publishedVersion | en |
dc.citation.rank | aM21 | |
dc.identifier.rcub | https://hdl.handle.net/21.15107/rcub_ibiss_1356 |