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Agrobacterium-mediated transformation of two Serbian potato cultivars (Solanum tuberosum L. cv. Dragacevka and cv. Jelica)

dc.creatorCingel, Aleksandar
dc.creatorVinterhalter, Branka
dc.creatorVinterhalter, Dragan V
dc.creatorĆalić-Dragosavac, Dušica D.
dc.creatorSmigocki, Ann
dc.creatorNinković, Slavica
dc.date.accessioned2017-11-23T11:10:47Z
dc.date.available2015-11-17T10:26:51Z
dc.date.issued2010sr
dc.identifier.issn1684-5315sr
dc.identifier.otherRad_konverzija_3355sr
dc.identifier.urihttps://radar.ibiss.bg.ac.rs/handle/123456789/1360
dc.description.abstractAn efficient protocol for Agrobacterium-mediated transformation of Serbian potato cultivars Dragacevka and Jelica, enabling the introduction of oryzacystatin genes OCI and OCII, was established. Starting with leaf explants, a two-stage transformation protocol combining procedures of Webb and Wenzler provided high shoot regeneration efficiency: 84 - 89% for Dragacevka cultivar and 60 - 68% for Jelica cultivar as compared to 76 - 86% for Desiree, the most frequently used cultivar in transformation experiments. PCR analysis of a small sample of putative transformants showed a nptII integration frequency of 90.9, 76.9 and 86.4% for Dragacevka, Jelica and Desiree, respectively. Regeneration and transformation efficiency was strongly genotype-dependent.en
dc.description.sponsorshipSerbian Ministry of Science and Technology [143026B]sr
dc.language.isoEnglishsr
dc.rightsrestrictedAccess
dc.sourceAfrican Journal of Biotechnologysr
dc.titleAgrobacterium-mediated transformation of two Serbian potato cultivars (Solanum tuberosum L. cv. Dragacevka and cv. Jelica)en
dc.typearticle
dc.rights.licenseARR
dcterms.abstractВинтерхалтер, Драган В; Цингел, Aлександар Д.; Ћалић-Драгосавац, Душица Д.; Нинковић, Славица Б.; Смигоцки, Aнн; Винтерхалтер, Бранка С.;
dc.citation.issue30sr
dc.citation.volume9sr
dc.citation.epage4650sr
dc.type.versionpublishedVersionen
dc.citation.rankM23
dc.identifier.rcubhttps://hdl.handle.net/21.15107/rcub_ibiss_1360


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