Kultura in vitro segmenata stabla kao metod za mikropropagaciju hrizantema

Abstract

The effect of several nutritional media on in vitro shoot multiplication of 13 chrysanthemum cultivars grown in our country was investigated. Medium supplemented with a-naphtalenacetic acid and 6-benzylaminopurine was the most efficient for shoot multiplication. The highest shoot multiplication index was achieved for cultivar 'Tigerrag'. Successful rooting of shoots, depending on cultivars, was obtained on a hormone free medium. Acclimatization of 'in vitro' plants was 55-84%. After growing in the field under suitable photoperiod regime, all cultivars flowered.

Proučavan je uticaj nekoliko hranljivih podloga na in vitro umnožavanje izdanaka 13 sorti hrizantema koje se gaje u našoj zemlji. Hranljive podloge obogaćene a-naftil sirćetnom i benzil-aminopurinom su bile najefikasnije za mikropropagaciju izdanaka. Najveći indeks umnožavanja izdanaka je postignut kod sorte 'Tigerrag'. Uspešno ožiljavanje (81-100%), u zavisnosti od sorte, je postignuto na hranljivoj podlozi bez hormona. Aklimatizacija 'in vitro' biljčica hrizantema se kretala u opsegu od 55-84%. Posle gajenja u uslovima polja pri odovarajućem fotoperiodu sve sorte su cvetale.