L-Arginine supplementation induces glutathione synthesis in interscapular brown adipose tissue through activation of glutamate-cysteine ligase expression: The role of nitric oxide
2009
Authors:
Otašević, VesnaBuzadžić, Biljana J.
Korac, Aleksandra B
Vasilijević, Ana
Janković, Aleksandra
Korać, Bato
Document Type:
Article (Published version)
,
© 2009 Elsevier Ireland Ltd
Metadata
Show full item recordAbstract:
We examined whether nitric oxide (NO) in vivo could induce interscapular brown adipose tissue (IBAT) glutathione synthesis. Data show that NO induces in vivo IBAT glutathione synthesis through activation of glutamate-cysteine ligase (GCL) mRNA and protein expression. This NO effect appeared to be mediated by nuclear factor-kappa B (NF-kappa B) activation. We have also observed a complex series of in vivo cellular responses during chronic inhibition of NO synthesis, suggesting that regulatory pathways unrelated to GCL alteration underlie glutathione level increase induced by N(omega)-nitro-L-arginine methyl ester (L-NAME). In general, glutathione synthesis in IBAT seemed to be finely tuned by NO to provide glutathione for either mediating the effects of NO, or for preventing potential nitrosative stress. (C) 2009 Elsevier Ireland Ltd. All rights reserved.
Source:
Chemico-Biological Interactions, 2009, 182, 2-3, 204-212
DOI: 10.1016/j.cbi.2009.07.010
ISSN: 0009-2797
PubMed: 19631198