Nitric oxide regulates mitochondrial re-modelling in interscapular brown adipose tissue: ultrastructural and morphometric-stereologic studies
2008
Autori:
Otašević, VesnaKorac, Aleksandra B
Buzadžić, Biljana J.
Vasilijević, Ana
Janković, Aleksandra
Micunović, Ksenija
Korać, Bato
Tip dokumenta:
Članak u časopisu (Objavljena verzija)
,
© 2008 The Authors Journal compilation © 2008 The Royal Microscopical Society
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
As a complex, cell-specific process that includes both division and clear functional differentiation of mitochondria, mitochondriogenesis is regulated by numerous endocrine and autocrine factors. In the present ultrastructural study, in vivo effects of l-arginine-nitric oxide (NO)-producing pathway on mitochondriogenesis in interscapular brown adipose tissue (IBAT) were examined. For that purpose, adult Mill Hill hybrid hooded rats were receiving l-arginine, a substrate of NO synthases (NOSs), or N(omega)-nitro-l-arginine methyl ester (l-NAME), an inhibitor of NOSs, as drinking liquids for 45 days. All experimental groups were divided into two sub-groups - acclimated to room temperature and cold. IBAT mitochondria were analyzed by transmission electron microscopy and stereology. l-Arginine treatment acted increasing the number of mitochondrial profiles per cell profile, as well as volume fraction of mitochondria per cell volume in animals maintained at room temperature. Cold-induced enhancement of number of mitochondrial profiles per cell profile was additionally increased in l-arginine-treated rats. Ultrastructural examinations of l-arginine-treated cold-acclimated animals clearly demonstrated thermogenically active mitochondria (larger size, lamellar, more numerous and well-ordered cristae in their profiles), which however were inactive in l-arginine-receiving animals kept at room temperature (small mitochondria, tubular cristae). By contrast, l-NAME treatment of rats acclimated to room temperature induced mitochondrial alterations characterized by irregular shape, short disorganized cristae and megamitochondria formation. These results showed that NO is a necessary factor for mitochondrial biogenesis and that it acts intensifying this process, but NO alone is not a sufficient stimulus for in vivo induction of mitochondriogenesis in brown adipocytes.
Izvor:
Journal of Microscopy, 2008, 232, 3, 542-548
DOI: 10.1111/j.1365-2818.2008.02132.x
ISSN: 0022-2720
PubMed: 19094038