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Nitric oxide regulates mitochondrial re-modelling in interscapular brown adipose tissue: ultrastructural and morphometric-stereologic studies
dc.creator | Otašević, Vesna | |
dc.creator | Korac, Aleksandra B | |
dc.creator | Buzadžić, Biljana J. | |
dc.creator | Vasilijević, Ana | |
dc.creator | Janković, Aleksandra | |
dc.creator | Micunović, Ksenija | |
dc.creator | Korać, Bato | |
dc.date.accessioned | 2017-11-23T11:12:42Z | |
dc.date.available | 2900-01-01 | |
dc.date.issued | 2008 | sr |
dc.identifier.issn | 0022-2720 | sr |
dc.identifier.other | Rad_konverzija_3497 | sr |
dc.identifier.uri | https://radar.ibiss.bg.ac.rs/handle/123456789/1502 | |
dc.description.abstract | As a complex, cell-specific process that includes both division and clear functional differentiation of mitochondria, mitochondriogenesis is regulated by numerous endocrine and autocrine factors. In the present ultrastructural study, in vivo effects of l-arginine-nitric oxide (NO)-producing pathway on mitochondriogenesis in interscapular brown adipose tissue (IBAT) were examined. For that purpose, adult Mill Hill hybrid hooded rats were receiving l-arginine, a substrate of NO synthases (NOSs), or N(omega)-nitro-l-arginine methyl ester (l-NAME), an inhibitor of NOSs, as drinking liquids for 45 days. All experimental groups were divided into two sub-groups - acclimated to room temperature and cold. IBAT mitochondria were analyzed by transmission electron microscopy and stereology. l-Arginine treatment acted increasing the number of mitochondrial profiles per cell profile, as well as volume fraction of mitochondria per cell volume in animals maintained at room temperature. Cold-induced enhancement of number of mitochondrial profiles per cell profile was additionally increased in l-arginine-treated rats. Ultrastructural examinations of l-arginine-treated cold-acclimated animals clearly demonstrated thermogenically active mitochondria (larger size, lamellar, more numerous and well-ordered cristae in their profiles), which however were inactive in l-arginine-receiving animals kept at room temperature (small mitochondria, tubular cristae). By contrast, l-NAME treatment of rats acclimated to room temperature induced mitochondrial alterations characterized by irregular shape, short disorganized cristae and megamitochondria formation. These results showed that NO is a necessary factor for mitochondrial biogenesis and that it acts intensifying this process, but NO alone is not a sufficient stimulus for in vivo induction of mitochondriogenesis in brown adipocytes. | en |
dc.description.sponsorship | Ministry of Science, Republic of Serbia [143050]; [COST FA0602 Action] | sr |
dc.language.iso | English | sr |
dc.publisher | John Wiley and Sons | |
dc.rights | restrictedAccess | |
dc.source | Journal of Microscopy | sr |
dc.title | Nitric oxide regulates mitochondrial re-modelling in interscapular brown adipose tissue: ultrastructural and morphometric-stereologic studies | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Василијевић, Aна; Корац, Aлександра Б; Јанковић, Aлександра; Бузаджић, Биљана Ј.; Петровић, Весна; Мицуновић, Ксенија; Кораћ, Бато М.; | |
dc.rights.holder | © 2008 The Authors Journal compilation © 2008 The Royal Microscopical Society | |
dc.citation.issue | 3 | sr |
dc.citation.volume | 232 | sr |
dc.identifier.doi | 10.1111/j.1365-2818.2008.02132.x | |
dc.identifier.pmid | 19094038 | |
dc.identifier.scopus | 2-s2.0-57449099876 | |
dc.identifier.wos | 000261253200024 | |
dc.citation.spage | 542 | |
dc.citation.epage | 548 | sr |
dc.type.version | publishedVersion | en |
dc.citation.rank | M22 | |
dc.identifier.rcub | https://hdl.handle.net/21.15107/rcub_ibiss_1502 |