Early temporal changes in ecto-nucleotidase activity after cortical stab injury in rat
2008
Аутори:
Nedeljković, Nadežda N.Bjelobaba, Ivana
Lavrnja, Irena
Savić, Danijela
Peković, Sanja
Rakić, Ljubisav
Stojiljković, Mirjana B.
Тип документа:
Чланак у часопису (Објављена верзија)
,
© 2007 Elsevier B.V.
Метаподаци
Приказ свих података о документуАпстракт:
During a variety of insults to the brain adenine nucleotides are released in large quantities from damaged cells, triggering multiple cellular responses to injury. Here, we evaluated changes in extracellular ATP, ADP and AMP hydrolysis at different times (0-24 hours) after unilateral cortical stab injury (CSI) in adult rats. Results demonstrated that 24 hours following CSI, ATP and ADP hydrolyzing activities were not significantly altered in injured cortex. Based on calculated V (ATP)/V (ADP) ratio it was concluded that ATP/ADP hydrolysis was primarily catalyzed by NTPDase1 enzyme form. In contrast, AMP hydrolysis, catalyzed by 5'-nucleotidase, was significantly reduced at least 4 hours following CSI. Kinetic analysis and Lineweaver-Burk transformation of the enzyme velocities obtained over the range of AMP concentrations (0.05-1.50 mM) revealed that inhibition of 5'-nucleotidase activity after CSI was of the uncompetitive type. Taken together our data suggest that injured tissue has reduced potential for extracellular metabolism of adenine nucleotides in early stages after CSI.
Кључне речи:
ecto-nucleotidase; NTPDase; 5'-nucleotidase; cerebral cortex; brain injury; ratИзвор:
Neurochemical Research, 2008, 33, 5, 873-879Финансирање / пројекти:
- Интеракција глије и неурона у процесу опоравка након оштећења централног нервног система (RS-143005)
DOI: 10.1007/s11064-007-9529-0
ISSN: 0364-3190
PubMed: 17992570