Early temporal changes in ecto-nucleotidase activity after cortical stab injury in rat
2008
Autori:
Nedeljković, Nadežda N.Bjelobaba, Ivana
Lavrnja, Irena
Savić, Danijela
Peković, Sanja
Rakić, Ljubisav
Stojiljković, Mirjana B.
Tip dokumenta:
Članak u časopisu (Objavljena verzija)
,
© 2007 Elsevier B.V.
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
During a variety of insults to the brain adenine nucleotides are released in large quantities from damaged cells, triggering multiple cellular responses to injury. Here, we evaluated changes in extracellular ATP, ADP and AMP hydrolysis at different times (0-24 hours) after unilateral cortical stab injury (CSI) in adult rats. Results demonstrated that 24 hours following CSI, ATP and ADP hydrolyzing activities were not significantly altered in injured cortex. Based on calculated V (ATP)/V (ADP) ratio it was concluded that ATP/ADP hydrolysis was primarily catalyzed by NTPDase1 enzyme form. In contrast, AMP hydrolysis, catalyzed by 5'-nucleotidase, was significantly reduced at least 4 hours following CSI. Kinetic analysis and Lineweaver-Burk transformation of the enzyme velocities obtained over the range of AMP concentrations (0.05-1.50 mM) revealed that inhibition of 5'-nucleotidase activity after CSI was of the uncompetitive type. Taken together our data suggest that injured tissue has reduced potential for extracellular metabolism of adenine nucleotides in early stages after CSI.
Ključne reči:
ecto-nucleotidase; NTPDase; 5'-nucleotidase; cerebral cortex; brain injury; ratIzvor:
Neurochemical Research, 2008, 33, 5, 873-879Finansiranje / projekti:
- Interakcija glije i neurona u procesu oporavka nakon oštećenja centralnog nervnog sistema (RS-MESTD-MPN2006-2010-143005)
DOI: 10.1007/s11064-007-9529-0
ISSN: 0364-3190
PubMed: 17992570