Приказ основних података о документу
Purification and properties of major midgut leucyl aminopeptidase of Morimus funereus (Coleoptera, Cerambycidae) larvae
dc.creator | Bozić, Natasa M | |
dc.creator | Ivanović, Jelisaveta | |
dc.creator | Nenadović, Vera | |
dc.creator | Bergstroem, Joergen | |
dc.creator | Larsson, Thomas | |
dc.creator | Vujčić, Zoran M. | |
dc.date.accessioned | 2017-11-23T11:12:55Z | |
dc.date.available | 2015-11-17T10:26:51Z | |
dc.date.issued | 2008 | sr |
dc.identifier.issn | 1096-4959 | sr |
dc.identifier.other | Rad_konverzija_3537 | sr |
dc.identifier.uri | https://radar.ibiss.bg.ac.rs/handle/123456789/1542 | |
dc.description.abstract | The major leucyl aminopeptidase (LAP) from the midgut of Morimus funereus larvae was purified and characterised. Specific LAP activity was increased 292-fold by purification of the crude midgut extract. The purified enzyme had a pH optimum of 7.5 (optimum pH range 7.0-8.5) and preferentially hydrolysed p-nitroanilides containing hydrophobic amino acids in the active site, with the highest V-max /K-M ratio for leucine-p-nitroanilide (LpNA). Among a number of inhibitors tested, the most efficient were 1, 10-phenanthroline having a K-i value of 0.12 mM and cysteine with K-i value of 0.31 mM, while EGTA stimulated LAP activity. Zn2+, Mg2+ and Mn2+ all showed bi-modal effects on LAP activity (activated at low concentrations and inhibited at high concentrations). The purified LAP (after gel filtration on Superose 6 column) had molecular mass of 400 kDa with an isoelectric point of 6.2. Sodium dodecylsulphate-polyacrylamide gel electrophoresis (SDS-PAGE) revealed one band of 67 kDa, suggesting that the enzyme is a hexamer. Six peptide sequences from protein band were obtained using ESI/MS-MS analysis. Comparison of the obtained peptide sequences with the EMBL-EBI sequence analysis toolbox and the BLASTP database showed a high degree of identity with other insect aminopeptidases. (C) 2007 Elsevier Inc. All rights reserved. | en |
dc.description.sponsorship | null | sr |
dc.language.iso | English | sr |
dc.rights | restrictedAccess | |
dc.source | Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology | sr |
dc.title | Purification and properties of major midgut leucyl aminopeptidase of Morimus funereus (Coleoptera, Cerambycidae) larvae | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Ненадовић, Вера; Бергстроем, Јоерген; Вујчић, Зоран М.; Ларссон, Тхомас; Ивановић, Јелисавета; Бозић, Натаса М; | |
dc.citation.issue | 3 | sr |
dc.citation.volume | 149 | sr |
dc.citation.epage | 462 | sr |
dc.type.version | publishedVersion | en |
dc.citation.rank | M21 | |
dc.identifier.rcub | https://hdl.handle.net/21.15107/rcub_ibiss_1542 |
Документи
Датотеке | Величина | Формат | Преглед |
---|---|---|---|
Уз овај запис нема датотека. |