Приказ основних података о документу
Effects of plant genotype and bacterial strain on Agrobacterium tumefaciens-mediated transformation of Brassica oleracea L. var. capitata
dc.creator | Sretenović-Rajičić, Tatjana D. | |
dc.creator | Ninković, Slavica | |
dc.creator | Uzelac, Branka | |
dc.creator | Vinterhalter, Branka | |
dc.creator | Vinterhalter, Dragan V | |
dc.date.accessioned | 2017-11-23T11:14:59Z | |
dc.date.available | 2015-11-17T10:26:51Z | |
dc.date.issued | 2007 | sr |
dc.identifier.issn | 1021-4437 | sr |
dc.identifier.other | Rad_konverzija_3578 | sr |
dc.identifier.uri | https://radar.ibiss.bg.ac.rs/handle/123456789/1583 | |
dc.description.abstract | Two inbred lines of Brassica oleracea L. var. capitata were transformed with two Agrobacterium tumefaciens strains harboring resistance to herbicide Basta: AGL1/pDM805 and LBA4404/pGKB5 (LB5-1). Inoculated cotyledons and hypocotyls provided equally good explants and manifested a high percentage of shoot regeneration on MS medium supplemented with 1 mg/l benzyladenine and 0.5 mg/l indole-3-butyric acid. The P34I5 genotype was superior to P22I5 in shoot regeneration (48.1 vs. 26.9%), multiplication, and acclimation in the greenhouse (76 vs. 40%). A. tumefaciens AGL1/pDM805 provided more regenerated shoots per explant, especially in the case of cotyledon explants, and the higher transformation rate (up to 35% vs. up to 12%) as compared to LB5-1. Putative transformants survived spraying with 10-30 mg/l phosphinothricin. Transformation was confirmed by GUS assay and PCR analysis in T0 and T1 generations. | en |
dc.description.sponsorship | null | sr |
dc.language.iso | English | sr |
dc.rights | restrictedAccess | |
dc.source | Russian Journal of Plant Physiology | sr |
dc.title | Effects of plant genotype and bacterial strain on Agrobacterium tumefaciens-mediated transformation of Brassica oleracea L. var. capitata | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Сретеновић-Рајичић, Татјана Д.; Нинковић, Славица Б.; Винтерхалтер, Бранка С.; Винтерхалтер, Драган В; Узелац, Бранка Б; | |
dc.citation.issue | 5 | sr |
dc.citation.volume | 54 | sr |
dc.citation.epage | 658 | sr |
dc.type.version | publishedVersion | en |
dc.citation.rank | M23 | |
dc.identifier.rcub | https://hdl.handle.net/21.15107/rcub_ibiss_1583 |
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