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A comparative study of the effects of molsidomine and 3-morpholinosydnonimine on the redox status of rat erythrocytes and reticulocytes
dc.creator | Marković, Snezana D | |
dc.creator | Vukajlović, Miroslava Đ | |
dc.creator | Ognjanović, Branka I | |
dc.creator | Stajn, Andras S | |
dc.creator | Žikić, Radoslav V. | |
dc.creator | Saičić, Zorica | |
dc.creator | Radojičić, Ratko M. | |
dc.creator | Jones, David R | |
dc.creator | Spasić, Mihajlo | |
dc.date.accessioned | 2017-11-23T11:15:14Z | |
dc.date.available | 2015-11-17T10:26:51Z | |
dc.date.issued | 2007 | sr |
dc.identifier.issn | 0263-6484 | sr |
dc.identifier.other | Rad_konverzija_3597 | sr |
dc.identifier.uri | https://radar.ibiss.bg.ac.rs/handle/123456789/1602 | |
dc.description.abstract | After enzymic biotransformation, molsidomine (MO) acts via the metabolite 3-morpholmosydnonimine (SIN-1) through spontaneous liberation of nitric oxide (NO) and superoxide (O-2(.-)). The aim of this study was to compare the effects of MO and its active metabolite SIN-1 on the redox status of rat erythrocytes and reticulocytes. Rat erythrocyte as well as reticulocyte-rich red blood cell (RBC) suspensions were aerobically incubated (2h, 37 degrees C) without (control) or in the presence of different concentrations of MO or SIN-1. In rat erythrocytes, biotransformation of MO resulted in the production of NO and nitroxyl (NO-). Endogenous superoxide anion (O-2(.-)) participated in peroxynitrite generation. SIN-1 simultaneously liberated NO and O-2(.-), which formed peroxynitrite (at least in part), but the liberated NO predominantly reacted with haemoglobin, forming methaemoglobin in erythrocytes. In reticulocytes, MO and SIN-1 caused an increase in the levels of both nitrite and 3-nitrotyrosine (an indicator of peroxynitrite), whereas they decreased the level of O-2(.-). In reticulocytes, MO was metabolized into SIN-1 which led to the generation of NO, which reacted with O-2(.-) (endogenous or exogenous) forming reactive nitrogen species. In conclusion, there are two metabolic pathways for MO biotransformation: one causing NO and NO- generation predominantly in erythrocytes and the other, via SINA metabolism, in reticulocytes. The main difference between the action of MO and SIN-1 was that the latter caused oxidative damage in RBCs. Copyright (c) 2006 John Wiley & Sons, Ltd. | en |
dc.description.sponsorship | null | sr |
dc.language.iso | English | sr |
dc.rights | restrictedAccess | |
dc.source | Cell Biochemistry and Function | sr |
dc.title | A comparative study of the effects of molsidomine and 3-morpholinosydnonimine on the redox status of rat erythrocytes and reticulocytes | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Стајн, Aндрас С; Спасић, Михајло Б.; Саичић, Зорица; Марковић, Снезана Д; Вукајловић, Мирослава Ђ; Огњановић, Бранка И; Жикић, Радослав В.; Радојичић, Ратко М.; Јонес, Давид Р; | |
dc.citation.issue | 3 | sr |
dc.citation.volume | 25 | sr |
dc.citation.epage | 258 | sr |
dc.type.version | publishedVersion | en |
dc.citation.rank | M23 | |
dc.identifier.rcub | https://hdl.handle.net/21.15107/rcub_ibiss_1602 |
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