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Nickel hyperaccumulation in shoot cultures of Alyssum markgrafii
dc.creator | Vinterhalter, Branka | |
dc.creator | Vinterhalter, Dragan V | |
dc.date.accessioned | 2017-11-23T11:15:20Z | |
dc.date.available | 2015-11-17T10:26:51Z | |
dc.date.issued | 2005 | sr |
dc.identifier.issn | 0006-3134 | sr |
dc.identifier.other | Rad_konverzija_3700 | sr |
dc.identifier.uri | https://radar.ibiss.bg.ac.rs/handle/123456789/1705 | |
dc.description.abstract | Shoot cultures of Alyssum markgrafii O.E. Shulz, endemic nickel hyperaccumulating species of central Balkan, were established and maintained on Murashige and Skoog medium supplemented with 0.2 mg dm(-3) benzyladenine (BA). Nickel in form of NiCl2 . 6 H2O was supplemented at 22 different concentrations ranging from 0.0001 to 15 mM but none of them was lethal to cultures. High Ni2+ concentrations (10 mM or more) arrested shoot growth which, upon transfer to Ni-free medium, commenced via axillary bud proliferation. Shoots that developed from axillary buds through the subculture manifested increased tolerance to Ni2+ expressed as shoot elongation. Shoot multiplication and dry biomass production decreased with increase of N2+ in medium. Only the accumulation of Ni2+ in tissues increased with Ni2+ content of the medium. Apart from shoot cultures, high Ni2+ accumulation was registered in undifferentiated callus cultured on medium with 0.5 mg dm(-3) BA and 0.5 mg dm(-3) naphthylacetic acid. Highest content of accumulated Ni was 2.37 mu g g(-1) (d.m.) in shoots and 2.65 mu g g(-1) (d.m.) in callus, both measured on medium with 15 mM Ni2+. | en |
dc.description.sponsorship | null | sr |
dc.language.iso | English | sr |
dc.rights | restrictedAccess | |
dc.source | Biologia Plantarum | sr |
dc.title | Nickel hyperaccumulation in shoot cultures of Alyssum markgrafii | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Винтерхалтер, Бранка С.; Винтерхалтер, Драган В; | |
dc.citation.issue | 1 | sr |
dc.citation.volume | 49 | sr |
dc.citation.epage | 124 | sr |
dc.type.version | publishedVersion | en |
dc.citation.rank | M22 | |
dc.identifier.rcub | https://hdl.handle.net/21.15107/rcub_ibiss_1705 |
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