In vitro regeneration and transformation of Blackstonia perfoliata

Abstract

In vitro root culture of yellow wort (Blackstonia perfoliata ( L.) Huds.) was initiated on Murashige and Skoog ( MS) medium. In the presence of benzylaminopurine ( BAP) numerous adventitious buds formed, which developed into shoots. Presence of indole-3-butyric acid ( IBA) in media significantly decreased number of buds, but increased development of lateral roots. On hormone-free medium shoots successfully rooted and developed flowers and viable seeds that formed another generation. Shoot cultures of B. perfoliata inoculated with suspension of Agrobacterium rhizogenes strain A4M70GUS developed hairy roots at 3 weeks and they were cultured on hormone-free MS medium. Spontaneous shoot regeneration occurred in 3 clones.