The thyroid C cells of ovariectomized rats treated with estradiol
2003
Аутори:
Filipović, BrankoŠošić-Jurjević, Branka
Nestorović, Nataša
Manojlović-Stojanoski, Milica
Kostić, Nada D
Milošević, Verica
Sekulić, Milka I.
Тип документа:
Чланак у часопису (Објављена верзија)
,
© 2003, Springer-Verlag
Метаподаци
Приказ свих података о документуАпстракт:
The structure and function of thyroid C cells were studied in ovariectomized (Ovx) adult female rats without and after chronic treatment with estradiol dipropionate (EDP). A peroxidase-antiperoxidase method was applied for localization of calcitonin (CT) in the C cells. Morphometric changes in their volume, nuclei, and relative volume density were evaluated in comparison with sham-operated control rats using a stereological method. The number of C cells was calculated. CT content in the sera was determined by radioimmunoassay. Ovariectomy (Ovx) led to a 21% increase in body weight (P<0.005), while treatment of Ovx rats with EDP decreased body weight by 25% (P<0.01). The immunoreactivity for CT in C cells of the Ovx rats was markedly increased. Significant decreases in the volume of C cells (by 13%; P<0.05) and serum CT (by 45%) were recorded, while the C cell number increased by 59% (P<0.05) in relation to the corresponding controls. The treatment of Ovx rats with EDP caused conspicuous degranulation of the C cells. The cellular volume was increased by 11% and serum CT by 36% in comparison with Ovx animals. At the same time a decrease in C cell number by 29% (P<0.05) was evident. It may be concluded that estradiol deficiency after Ovx reduced the synthesis and release of CT, while chronic treatment of these animals with EDP had a positive effect on the secretory activity of thyroid C cells.
Извор:
Histochemistry and Cell Biology, 2003, 120, 5, 409-414
DOI: 10.1007/s00418-003-0578-4
ISSN: 0948-6143
PubMed: 14564528