Direct regeneration of shoots from hairy root cultures of Centaurium erythraea inoculated with Agrobacterium rhizogenes
Abstract:
Stable transformation and expression of transgenes was achieved in Centaurium erythraea Gillib. using Agrobacterium rhizogenes-mediated system. Five hairy root clones exhibited the transformed phenotype. Shoot regeneration, with green organized structures, was apparent in 4 clones, after the first subculture on Murashige and Skoog (MS) half strength medium. The integration of Ri-plasmid T-DNA was confirmed by polymerase chain reaction (PCR) analyses.