Kora nadbubrežne žlezde peripubertalnih i odraslih ženki pacova posle neonatalnog tretmana sa SRIH-14

Abstract

Neonatal female rats (3-7 days old) were injected subcutaneously twice daily with 20 μg of SRIH-14/100g b.w. for five consecutive days. The animals were sacrificed in the peripubertal (38 days old) or adult (80 days old) periods of life. Control rats were treated in the same way with identical volumes of saline. SRIH-14 led to significant reduction (p<0.05) of the absolute and relative volumes of the ZG in peripubertal animals, by 11 and 22%, respectively in comparison with the controls. The total number and volume of ZG cells and their nuclei were also significantly decreased (p<0.05) in peripubertal SRIH-14-treated rats as compared to the controls. There were no significant (p>0.05) changes in the morphometric parameters of adult adrenal cortex after SRIH-14 treatment compared with control females. It can be concluded that subcutaneously applied SRIH-14 in the neonatal period inhibits growth of adrenal ZG cells in female rats only up to the peripubertal stage, while the adrenal gland cortex recovers before mature adulthood is reached.

Ženke pacova od 3-7. dana starosti (neonatalni period) su subkutano tretirane dva puta dnevno sa SRIH-14 u dozi od 20 μg na 100 g telesne mase tokom pet uzastopnih dana. Životinje su bile žrtvovane u peripubertalnom (38. dana) ili odraslom (80. dana) periodu života. Kontrolne grupe su na identičan način tretirane fiziološkim rastvorom. U poređenju sa kontrolnim vrednostima tretman sa SRIH-14 je značajno smanjio (p<0.05) apsolutni (-11%) i relativni (-22%) volumenzone glomeruloze (ZG) u peripubertalnih životinja. Ukupan broj i zapremina ćelija ZG i njihovih jedara takođe su značajno smanjeni (p<0.05) u pacova tretiranih sa SRIH-14 u peripubertalnom periodu života u poređenju sa kontrolnim ženkama. U kori nadbubrežne žlezde odraslih ženki pacova nisu zapažene (p>0.05) promene morfometrijskih parametrima posle SRIH-14 tretmana u poređenju sa odgovarajućom kontrolom. Na osnovu dobijenih rezultata možemo zaključiti da je subkutano tretiranje ženki pacova u neonatalnom periodu sa SRIH-14 inhibiralo rast zone glomeruloze u peripubertalnih pacova.