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Cell-specific inhibition of inducible nitric oxide synthase activation by leflunomide
dc.creator | Janković, V | |
dc.creator | Samardžić, Tatjana S. | |
dc.creator | Stošić-Grujičić, Stanislava | |
dc.creator | Popadić, Dusan M | |
dc.creator | Trajković, Vladimir S | |
dc.date.accessioned | 2017-11-23T11:15:42Z | |
dc.date.available | 2015-11-17T10:26:51Z | |
dc.date.issued | 2000 | sr |
dc.identifier.issn | 0008-8749 | sr |
dc.identifier.other | Rad_konverzija_3829 | sr |
dc.identifier.uri | https://radar.ibiss.bg.ac.rs/handle/123456789/1834 | |
dc.description.abstract | The influence of a novel immunomodulating drug, leflunomide, on iNOS-dependent nitric oxide (NO) production in rodent macrophages and fibroblasts was investigated. Leflunomide's active metabolite A77 1726 caused a dose-dependent decrease of NO production in IFN-gamma-treated L929 fibroblasts. The observed effect was cell-specific, as well as stimulus-specific, since A77 1726 did not affect NO production in IFN-gamma-stimulated murine peritoneal macrophages or db-cAMP-treated L929 cells. A77 1726 reduced expression of IFN-gamma-induced iNOS and IRF-1 mRNA in L929 cells, while iNOS enzymatic activity remained unchanged. Specific inhibitor of MAP kinase kinase (MEK), PD98059, but not unselective protein kinase inhibitor genistein, completely mimicked cell-type-specific and stimulus-specific NO-inhibitory action of leflunomide. Therefore, the recently described inhibition of MEK/MAP pathway by leflunomide could present a possible mechanism for its suppression of iNOS activation in L929 fibroblasts. Finally, a similar inhibitory effect of A77 1726 on both NO production and iNOS mRNA expression was observed also in IFN-gamma + LPS-activated murine and rat primary fibroblasts. (C) 2000 Academic Press. | en |
dc.description.sponsorship | null | sr |
dc.language.iso | English | sr |
dc.rights | restrictedAccess | |
dc.source | Cellular Immunology | sr |
dc.title | Cell-specific inhibition of inducible nitric oxide synthase activation by leflunomide | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Попадић, Дусан М; Стошић-Грујичић, Станислава Д.; Јанковић, В; Самарджић, Татјана С.; Трајковић, Владимир С; | |
dc.citation.issue | 2 | sr |
dc.citation.volume | 199 | sr |
dc.citation.epage | 80 | sr |
dc.type.version | publishedVersion | en |
dc.citation.rank | M22 | |
dc.identifier.rcub | https://hdl.handle.net/21.15107/rcub_ibiss_1834 |
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