Glutation kao pogodan biomarker u hepatopankreasu, škrgama i mišićima tri vrste slatkovodnih rakova

Abstract

We determined the contents of total glutathione (tGSH), reduced glutathione (GSH), and oxidized glutathione (GSSG) and values of the glutathione redox index (GSH RI) in hepatopancreas, gills, and muscle of three freshwater crayfish species: noble crayfish (Astacus astacus) from the Southern Morava River, stone crayfish (Austropotamobius torrentium) from the Krajkovačka River, and spinycheek crayfish (Orconectes limosus) from the Danube River. The obtained data show strong tissue and species specifity of investigated parameters: tGSH, GSH, GSSG, and GSH RI in the hepatopancreas, gills, and muscle of the indicated crayfish species. Our work represents the first study of its kind and showed that the investigated parameters can be considered suitable biomarkers of the cellular glutathione redox status in of freshwater crayfish species.

Određivan je sadržaj ukupnog glutationa (tGSH),redukovanog glutationa (GSH),oksidovanog glutationa (GSSG)i glutation redoks indeksa (GSH RI)u hepatopankreasu, škrgama i mišićima tri vrste slatkovodnih rakova: rečnog raka (Astacus astacus) iz reke Južna Morava, raka kamenjara (Austropotamobius torrentium) iz Krajkovačke reke i američkog raka (Orconecteslimosus) iz reke Dunav. Dobijeni podaci pokazuju značajnu tkivnu i specijes specifičnost ispitivanih parametara: GSH, GSSG i GSHRI u hepatopankreasu, škrgama i mišićima kod rakova. Naš rad predstavlja prvu studiju ove vrste i pokazuje da ispitivani parametri mogu biti pogodni biomarkeri ćelijskog redoks statusa glutationa kod slatkovodnih vrsta rakova.