Strain differences in the immune mechanisms of resistance of immunocompetent rats to pulmonary aspergillosis
2015
Аутори:
Mirkov, IvanaDemenesku, Jelena
Popov Aleksandrov, Aleksandra
Ninkov, Marina
Glamočlija, Jasmina
Kataranovski, Dragan S.
Kataranovski, Milena
Тип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
Although the relevance of genetically-based variations in susceptibility
to pulmonary aspergillosis was shown in immunocompromised mice and is
indicated in humans, there is virtually no information concerning
variations in antifungal immune responses in resistant individuals. We
have shown recently the relevance of proinflammatory cytokine
(interferon-gamma/IFN-gamma and interleukin-17/IL-17) responses in
resistance to sublethal Aspergillus fumigatus infection of
non-suppressed Dark Agouti (DA) rats (strain known of a substantial
immune reactivity to noxious insults). In this study, anti-fungal immune
activities of leukocytes recovered from lungs by enzyme digestion
(phagocytosis, oxidative activity, hyphal killing, CD11b expression, as
well as production of IFN-gamma, IL-17 and Th2/anti-inflammatory
cytokines interleukin-4/IL-4 and interleukin-10/IL-10) were investigated
in less reactive Albino Oxford (AO) and compared to DA rats. Elimination
of fungus from lungs of AO rats was associated with lower degree of
leukocyte infiltration and of the majority of their basic effector
activities in comparison to DA rats. Lower production of IFN-gamma by
pulmonary leukocytes was observed early (day 1) post infection (p.i.) in
AO compared to DA rats, but without changes in IL-4. Both strains
responded to infection by an increase of IL-17 and IL-10, but production
of cytokines was higher (from days 7 p.i. and 3 p.i. for IL-17 and
IL-10, respectively) in AO compared to DA rats. The levels and pattern
of IFN-gamma and IL-4 responses by draining lymph node (dLN) cells were
similar in both strains and basically corresponded to those of lung
leukocytes. In contrast, similar levels of draining lymph node cell
production of IL-17 and IL-10 were observed in both strains with lack of
changes in mRNA, what suggests additional stimulation of these cytokines
in lungs of AO rats. The knowledge of strain differences in the
immune-based strategies in response of immunocompetent hosts to A.
fumigatus might contribute to our understanding of variations in
underlying mechanisms that enable of resistance to this fungus. (C) 2015
Elsevier GmbH. All rights reserved.
Кључне речи:
A. fumigatus pulmonary infection; Lung leukocytesИзвор:
Immunobiology, 2015, 220, 9, 1075-1084
DOI: 10.1016/j.imbio.2015.05.007
ISSN: 0171-2985