Cell death of spinal cord ED1(+) cells in a rat model of multiple sclerosis
2015
Аутори:
Trifunovic, DraganaNikolovski, Neda
Lavrnja, Irena
SophieWendrich, Katrin
Paquet-Durand, Francois
Miljković, Đorđe
Тип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
Infiltration of macrophages into the central nervous system and
activation of microglia are hallmarks of multiple sclerosis and its
animal model-experimental autoimmune encephalomyelitis (EAE). Cell death
in EAE has been demonstrated as an essential mechanism in the local
regulation of the inflammatory reaction, but also as one of the major
factors contributing to the destruction of the nervous tissue. The focus
of this study was on detection of cell death among ED1(+) cells
(macrophages/activated microglia) in the spinal cord of Dark Agouti rats
at the peak of EAE. Cell death was assessed using the TUNEL assay and
immunostaining for cleaved caspase 3, as markers for cell death in
general and ``classical{''} apoptosis, respectively. Major infiltrates
of immune cells were detected both in white matter and gray matter of
spinal cords in rats at the disease peak. ED1, TUNEL, and caspase 3
positive cells were detected within, but also outside the infiltrates.
There were more dying ED1+ cells in white matter than in gray matter,
both in the general population and in infiltrated regions. The observed
discrepancy in the proportion of dying ED1+ cells in spinal cord gray
and white matter indicated that in EAE rat macrophages/microglia within
gray matter are less prone to cell death induction. This is of special
interest in the context of the increasingly appreciated contribution of
spinal cord gray matter inflammation to multiple sclerosis pathogenesis.
Our findings suggest that activated macrophages/microglia of gray matter
are less susceptible to cell death induction. Alternatively, it can be
assumed that intrinsic cell death-inductive mechanisms of nervous tissue
differ in white and gray matter. Thus, further research on the gray
matter macrophages/microglia cell death during EAE is warranted. They
should be aimed at identification of the reasons for the observed
differences and finding suitable ways to stimulate gray matter activated
macrophages/microglia death.
Кључне речи:
Cell death; Gray matter; Caspase 3; Macrophage; MicrogliaИзвор:
Peerj, 2015, 3, 3:e1189
DOI: 10.7717/peerj.1189
ISSN: 2167-8359