Cordyceps militaris (L.) Link Fruiting Body Reduces the Growth of a Non-Small Cell Lung Cancer Cell Line by Increasing Cellular Levels of p53 and p21
2015
Аутори:
Bizarro, AnaFerreira, Isabel C. F. R.
Soković, Marina
van Griensven, Leo J. L. D.
Sousa, Diana
Vasconcelos, M. Helena
Lima, Raquel T.
Тип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
Cordyceps militaris (L.) Link, an edible entomopathogenic fungus widely
used in traditional Chinese medicine, has numerous potential medicinal
properties including antitumor activity. The methanolic extract of C.
militaris fruiting body was recently shown to have tumor cell growth
inhibitory activity in several human tumor cell lines. Nonetheless, the
mechanism of action involved is still not known. This work aimed at
further studying the effect of the methanolic extract of C. militaris
regarding its antitumor mechanism of action, using the non-small cell
lung cancer cell line (NCI-H460) as a model. Results showed that
treatment with the extract decreased cellular proliferation, induced
cell cycle arrest at G0/G1 and increased apoptosis. In addition, the
extract increased the levels of p53 and p21. Moreover, an increase in
p-H2A.X and 53BP1 levels, together with an increase in the number of
53BP1 foci/cell (all indicative of DNA damage), were also observed after
treatment with the extract. This work suggests that this extract
affected NCI-H460 cellular viability through a mechanism involving DNA
damage and p53 activation. This further supports the potential of this
extract as a source of bioactive compounds, which may be used in
anticancer strategies.
Кључне речи:
Cordyceps militaris methanolic extract; cell cycle arrestИзвор:
Molecules, 2015, 20, 8, 13927-13940
DOI: 10.3390/molecules200813927
ISSN: 1420-3049