Micro RNA-155 participates in re-activation of encephalitogenic T cells
2015
Аутори:
Jevtić, BojanTimotijevic, Gordana
Stanisavljević, Suzana
Momčilović, Miljana
Stojkovic, Marija Mostarica
Miljković, Đorđe
Тип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
MicroRNAs (miR) are small non-coding RNAs involved in the immune
response regulation. miR-155 has been attributed a major
pro-inflammatory role in the pathogenesis of multiple sclerosis and its
animal model experimental autoimmune encephalomyelitis (EAE). Here, a
role of miR-155 in re-activation of encephalitogenic CD4(+) T cells was
investigated. Dark Agouti rats were immunized with myelin basic protein
(MBP) emulsified in complete Freund's adjuvant. CD4(+) T cells were
purified from draining lymph node cells (DLNC) obtained in the inductive
phase and from spinal cord immune cells (SCIC) isolated at the peak of
EAE. CD4(+) T cells obtained from SCIC (i.e., in vivo re-activated
cells) had markedly higher expression of miR-155 in comparison to those
purified from DLNC (not re-activated). Likewise, in vitro re-activation
of DLNC with MBP led to increase in miR-155 expression. Further, DLNC
and DLNC CD4(+) T cells were transfected with an inhibitor of miR-155
during in vitro re-activation. As a result, expression of important
CD4(+) T cell effector cytokines IFN-gamma and IL-17, but not of
regulatory cytokines IL-10 and TGF-beta, was reduced. These results
imply that miR-155 supports re-activation of encephalitogenic CD4+ T
cells. Our results contribute to a view that miR-155 might be a valuable
target in multiple sclerosis therapy. (C) 2015 Elsevier Masson SAS. All
rights reserved.
Кључне речи:
micro RNA; Cytokines; Autoimmunity; Central nervous systemИзвор:
Biomedicine & Pharmacotherapy, 2015, 74, 206-210
DOI: 10.1016/j.biopha.2015.08.011
ISSN: 1950-6007