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Micro RNA-155 participates in re-activation of encephalitogenic T cells
dc.creator | Jevtić, Bojan | |
dc.creator | Timotijevic, Gordana | |
dc.creator | Stanisavljević, Suzana | |
dc.creator | Momčilović, Miljana | |
dc.creator | Stojkovic, Marija Mostarica | |
dc.creator | Miljković, Đorđe | |
dc.date.accessioned | 2016-05-23T10:59:40Z | |
dc.date.issued | 2015 | |
dc.identifier.issn | 1950-6007 | |
dc.identifier.uri | https://radar.ibiss.bg.ac.rs/handle/123456789/1919 | |
dc.description.abstract | MicroRNAs (miR) are small non-coding RNAs involved in the immune response regulation. miR-155 has been attributed a major pro-inflammatory role in the pathogenesis of multiple sclerosis and its animal model experimental autoimmune encephalomyelitis (EAE). Here, a role of miR-155 in re-activation of encephalitogenic CD4(+) T cells was investigated. Dark Agouti rats were immunized with myelin basic protein (MBP) emulsified in complete Freund's adjuvant. CD4(+) T cells were purified from draining lymph node cells (DLNC) obtained in the inductive phase and from spinal cord immune cells (SCIC) isolated at the peak of EAE. CD4(+) T cells obtained from SCIC (i.e., in vivo re-activated cells) had markedly higher expression of miR-155 in comparison to those purified from DLNC (not re-activated). Likewise, in vitro re-activation of DLNC with MBP led to increase in miR-155 expression. Further, DLNC and DLNC CD4(+) T cells were transfected with an inhibitor of miR-155 during in vitro re-activation. As a result, expression of important CD4(+) T cell effector cytokines IFN-gamma and IL-17, but not of regulatory cytokines IL-10 and TGF-beta, was reduced. These results imply that miR-155 supports re-activation of encephalitogenic CD4+ T cells. Our results contribute to a view that miR-155 might be a valuable target in multiple sclerosis therapy. (C) 2015 Elsevier Masson SAS. All rights reserved. | en |
dc.description.sponsorship | Ministry of Education, Science and Technological Development of the Republic of Serbia {[}173035, 175038, 173013] | |
dc.language | English | |
dc.rights | restrictedAccess | |
dc.source | Biomedicine & Pharmacotherapy | |
dc.subject | micro RNA | |
dc.subject | Cytokines | |
dc.subject | Autoimmunity | |
dc.subject | Central nervous system | |
dc.title | Micro RNA-155 participates in re-activation of encephalitogenic T cells | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Стојковиц, Марија Мостарица; Миљковић, Ђорђе М.; Тимотијевиц, Гордана; Момчиловић, Миљана Б.; Јевтић, Бојан; Станисављевић, Сузана; Мицро РНA-155 партиципатес ин ре-ацтиватион оф енцепхалитогениц Т целлс; | |
dc.citation.volume | 74 | |
dc.identifier.doi | 10.1016/j.biopha.2015.08.011 | |
dc.identifier.scopus | 2-s2.0-84940838745 | |
dc.identifier.wos | 000361182600031 | |
dc.citation.spage | 206 | |
dc.citation.epage | 210 | |
dc.type.version | publishedVersion | en |