Growth and development of Colorado potato beetle larvae, Leptinotarsa decemlineata, on potato plants expressing the oryzacystatin II proteinase inhibitor
2015
Аутори:
Cingel, AleksandarSavic, Jelena
Vinterhalter, Branka
Vinterhalter, Dragan
Kostic, Miroslav
Šešlija Jovanović, Darka
Smigocki, Ann
Ninković, Slavica
Тип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
Plant proteinase inhibitors (PIs) are attractive tools for crop
improvement and their heterologous expression can enhance insect
resistance in transgenic plants. PI oryzacystatin II (OCII), isolated
from rice, showed potential in controlling pests that utilize cysteine
proteinases for protein digestion. To evaluate the applicability of the
OCII gene in enhancing plant defence, OCII-transformed potatoes were
bioassayed for resistance to Colorado potato beetle (Leptinotarsa
decemlineata Say). Feeding on transformed leaves of potato cultivars
Desiree and Jelica significantly affected larval growth and development,
but did not change mortality rates. During the L2 and L3 developmental
stages larvae consumed the OCII-transformed foliage faster as compared
to the nontransformed control. Also these larvae reached the prepupal
stage (end of L4 stage) 2 days earlier than those fed on control leaves.
However, the total amounts of consumed OCII-transformed leaves were up
to 23 \% lower than of control, and the maximal weights of prepupal
larvae were reduced by up to 18 \% as compared to larvae fed on
nontransformed leaves. The reduction in insect fitness reported in this
study in combination with other control measures, could lead to improved
CPB resistance management in potato.
Кључне речи:
Solanum tuberosum; Proteinase inhibitor; Oryzacystatin II; Colorado potato beetle; Pest managementИзвор:
Transgenic Research, 2015, 24, 4, 729-740
DOI: 10.1007/s11248-015-9873-9
ISSN: 1573-9368