Effects of fluoranthene on the fitness-related traits and antioxidative defense in Lymantria dispar L.

Abstract

This study aimed to examine the effects of ubiquitous polycyclic aromatic hydrocarbon fluoranthene, supplemented to an artificial diet, on the fitness-related traits and activity of midgut antioxidative enzymes-superoxide dismutase (SOD) and catalase (CAT), and expression of their isoforms in the fifth-instar gypsy moth Lymantria dispar L. Prolonged duration of development and reduced weight and relative growth rate were recorded in larvae reared on the diets supplemented with different concentrations of fluoranthene. SOD and CAT activities were significantly higher in the midguts of fluoranthene-treated larvae, compared to that of the control group. Different expression patterns were detected for SOD as well as for CAT isoforms, depending on the supplemented concentration of fluoranthene. Obtained results suggest that the activity of these enzymes in gypsy moth larvae may be used as biomarkers for assessing pollution, even at low concentrations of the pollutant.