Glutathione-dependent enzyme activities and concentrations of glutathione, vitamin E and sulfhydryl groups in barbel (Barbus barbus) and its intestinal parasite Pomphorhynchus laevis (Acanthocephala)
2015
Аутори:
Radovanović, TijanaProkić, Marko
Gavrić, Jelena
Despotović, Svetlana
Gavrilović, Branka
Borković Mitić, Slavica
Pavlović, Slađan
Saičić, Zorica
Тип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
Barbel (Barbus barbus) is the final host of the adult worm
Pomphorhynchus laevis (Acanthocephala), one of the most abundant and
widespread intestinal parasites of European freshwater fish. During the
course of the present study, we analyzed the activities of the
glutathione-dependent enzymes glutathione peroxidase (GSH-Px),
glutathione reductase (GR) and the phase II biotransformation enzyme
glutathione-S-transferase (GST) as well as the concentrations of total
glutathione (GSH), sulfhydryl (-SH) groups and vitamin E (Vit E) in the
liver and intestine of B. barbus and in its intestinal parasite P.
laevis. The,fish were caught from the Danube River (Serbia) in spring
and summer. We detected that GSH-Px activity in fish liver was higher in
spring. GR activity was significantly higher in spring in all
investigated samples, while GST activity was significantly higher in
spring in fish liver and in the parasite. At that time, GST activity was
ten times higher in the parasite than in fish tissues. The concentration
of GSH was increased in barbel liver in spring. In summer, the
concentration of SH groups was significantly increased, while the
concentration of Vit E was significantly decreased in fish tissues and
in the parasite. We performed Canonical Discriminant Analysis, which
revealed differentiation among the examined tissues during both seasons
based on the all measured antioxidant components. We found that the
seasonal patterns of antioxidant defense in the parasite are closely
correlated with seasonal variation and physiological change in the host
and represent the parasite's adaptation to changes in the host's
antioxidant system. The present investigation contributes to general
knowledge and provides a basis for future studies of
glutathione-dependent enzymes and non-enzymatic low molecular mass
antioxidants as potential biomarkers for monitoring the influence of the
environment on fishes and their parasites. (C)2015 Elsevier Ltd. All
rights reserved.
Кључне речи:
Barbus barbus; Pomphorhynchus laevis; Glutathione-dependent enzymes; Glutathione; Sulfhydryl groups; Vitamin EИзвор:
Ecological Indicators, 2015, 54, 31-38
DOI: 10.1016/j.ecolind.2015.02.005
ISSN: 1872-7034