Association between FokI, ApaI and TaqI RFLP polymorphisms in VDR gene and Hashimoto's thyroiditis: preliminary data from female patients in Serbia
2015
Аутори:
Djurovic, J.Stojkovic, O.
Ozdemir, O.
Silan, F.
Akurut, C.
Todorovic, J.
Savic, K.
Stamenković, Gorana
Тип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
Hashimoto's thyroiditis (HT) is the most prevalent autoimmune thyroid
disorder caused by an interaction between genes and environmental
triggers. Intrathyroid lymphocytic infiltration may lead to progressive
destruction of thyroid tissue and consequently to hypothyroidism. Many
studies in different populations have shown association between vitamin
D receptor (VDR) gene polymorphisms and various autoimmune diseases,
including HT. The study included 44 female patients (mean age +/-
standard deviation 38 +/- 5.4) with Hashimoto's thyroiditis and 32
healthy age-matched, sex-matched and geographically matched controls
without personal history of autoimmune and endocrine diseases. Genomic
DNA was isolated from peripheral blood-EDTA, and the target VDR gene was
genotyped by PCR-RFLP technique after VDR-FokI (rs2228570), VDR-ApaI
(rs7975232) and VDR-TaqI (rs731236) restriction enzymes digestion. We
used spss 20.0 integrated software for data analysis and found a
significant difference in the genotype distribution of VDR-FokI
polymorphism between patients with HT and controls (P=0.009). For ApaI
and TaqI, we observed a higher frequency of variant allele in patients
with HT, which was not significantly different compared to control women
(P>0.05). The current first and preliminary results identified the
association between VDR-FokI gene polymorphism and Hashimoto's
thyroiditis in Serbian population. Results need to be supported by
further investigations that define haplotype patterns for VDR gene
polymorphisms in a larger group of HT patients of both sexes.
Извор:
International Journal of Immunogenetics, 2015, 42, 3, 190-194
DOI: 10.1111/iji.12199
ISSN: 1744-313X