Gentiana dinarica Beck. hairy root cultures and evaluation of factors affecting growth and xanthone production
2015
Аутори:
Vinterhalter, BrankaKrstić Milošević, Dijana
Jankovic, Teodora
Pljevljakusic, Dejan
Ninković, Slavica
Smigocki, Ann
Vinterhalter, Dragan
Тип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
The induction and establishment of hairy root cultures of Gentiana
dinarica using two strains of Agrobacterium rhizogenes (A4M70GUS and
15834/PI) is reported for the first time. Strain 15834/PI had higher
induction rate of hairy roots (32.15 \%) than strain A4M70GUS (6.12 \%).
Transgenic nature of the roots was confirmed by GUS assay and PCR
analysis. Two clones per strain (A4M70GUS-D and -I, and 15834/PI-2 and
-3) with marked differences in general morphology and growth rate were
further studied. The methanol extracts of hairy root clones were
analyzed for xanthones content using HPLC method. The effects of the
type of carbohydrate source (sucrose, fructose or glucose) at different
concentrations on the growth parameters (growth index, dry weight,
fresh/dry weight ratio), phenolic and xanthone production, and free
radical scavenging activity of the transgenic clones were evaluated.
Statistical two level factorial design was used to define optimal
conditions for growth and successful secondary metabolite production in
G. dinarica hairy root clones. The results showed that clones A4M70GUS-D
and 15834/PI-3 were the superior ones. These two clones had the highest
dry weight on 116.8 mM sucrose, producing up to threefold higher amounts
of total phenolics and norswertianin-1-O-primeveroside than other
clones, untransformed roots and roots of wild-grown plants.
Кључне речи:
Gentiana dinarica; Agrobacterium rhizogenes; Carbohydrates; Xanthone; Total phenolicsИзвор:
Plant Cell Tissue and Organ Culture, 2015, 121, 3, 667-679
DOI: 10.1007/s11240-015-0737-z
ISSN: 1573-5044