Plant regeneration in leaf culture of Centaurium erythraea Rafn. Part 2: the role of arabinogalactan proteins
2015
Аутори:
Simonović, AnaFilipović, Biljana
Trifunović-Momčilov, Milana
Malkov, Sasa N.
Milinkovic, Vedrana P.
Jevremović, Slađana
Subotić, Angelina
Тип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
Arabinogalactan proteins (AGPs) are cell wall hydroxyproline-rich
glycoproteins with diverse functions in plant growth and development. In
this work the role of AGPs was comparatively studied in four
developmental pathways that can be induced from centaury leaf
explants-indirect somatic embryogenesis (ISE), indirect and direct shoot
development (ISD and DSD) and direct root development (DRD). The
addition of beta-d-glucosyl Yariv (beta GlcY) reagent, a synthetic
phenylglycoside that specifically crosslinks AGPs, to the growth medium
reduced the number of somatic embryos and adventitious buds formed per
explant. The morphogenetic paths can be arranged in order of increasing
sensitivity to beta GlcY as: DRD (insensitive) < ISD < DSD a parts per
thousand(a) ISE. The content of AGPs increased during all processes, but
the accumulation of AGPs was the most rapid during ISE and when ISE and
ISD occurred simultaneously. We have identified four centaury AGP
transcripts, CeAGP1-CeAGP4, of which CeAGP1, 2 and 4 code for
fasciclin-like AGPs. CeAGP3 is an AG peptide with conserved DUF1070
domain. Expression pattern of these genes indicated specific involvement
of CeAGP1 in ISE and unspecific involvement of CeAGP3 in morphogenesis.
Кључне речи:
Fasciclin; beta-D-Glucosyl YarivИзвор:
Plant Cell Tissue and Organ Culture, 2015, 121, 3, 721-739
DOI: 10.1007/s11240-015-0741-3
ISSN: 1573-5044