In vitro shoot organogenesis and comparative analysis of endogenous phytohormones in kohlrabi (Brassica oleracea var. gongylodes): effects of genotype, explant type and applied cytokinins
2015
Аутори:
Ćosić, TatjanaMotyka, Vaclav
Raspor, Martin
Savic, Jelena
Cingel, Aleksandar
Vinterhalter, Branka
Vinterhalter, Dragan
Travnickova, Alena
Dobrev, Petre I.
Bohanec, Borut
Ninković, Slavica
Тип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
Kohlrabi (Brassica oleracea var. gongylodes) cultivars Vienna Purple
(VP) and Vienna White (VW) were tested for their ability of de novo
organogenesis in vitro. Root, cotyledon, hypocotyl explants and intact
seedlings were cultivated on Murashige and Skoog (MS) media supplemented
with different cytokinins: benzyladenine (BA), thidiazuron (TDZ), trans-
or cis-zeatin. All tested cytokinins, including cis-zeatin, induced
shoot regeneration from hypocotyl explants and intact seedlings, with
seedlings being most successful for regeneration efficiency and
viability of regenerated shoots in both cultivars. The highest frequency
of shoot regeneration was achieved on MS with BA (60 \%) or TDZ (50 \%)
for VP; and with BA (50 \%), TDZ (47.5 \%) or transZ (37.5 \%) for VW.
Measurements of the endogenous cytokinin and indole-3-acetic acid (IAA)
contents in both hypocotyl explants and seedlings with regenerated
shoots (HRSs and SRSs) suggested that the observed differences in
organogenic response between these two types of explants were related to
their cytokinin and IAA contents. HRSs generally exhibited elevated
amounts of total cytokinins, while SRSs displayed a higher IAA/bioactive
cytokinins ratio. Shoots regenerated from seedlings were further
successfully multiplicated on a medium supplemented with BA (0.5 mg
L-1). The rooting potential of multiplicated shoots was tested on media
supplemented with 2 or 4 mg L-1 indole-3-butyric acid (IBA), with the
higher concentration of IBA leading to more efficient rooting. Rooted
plantlets were successfully planted into soil and flow cytometric
analysis did not reveal ploidy variations, indicating that the described
protocol is fast and efficient for kohlrabi regeneration.
Кључне речи:
Kohlrabi; Cytokinin; Auxin; Hypocotyl; Seedling; De novo shoot organogenesisИзвор:
Plant Cell Tissue and Organ Culture, 2015, 121, 3, 741-760
DOI: 10.1007/s11240-015-0742-2
ISSN: 1573-5044