Tamoxifen stimulates calcitonin-producing thyroid C-cells and prevents trabecular bone loss in a rat model of androgen deficiency
2015
Аутори:
Filipović, BrankoŠošić-Jurjević, Branka
Ajdžanović, Vladimir
Živanović, Jasmina
Isenović, Esma
Popovska-Perčinić, Florina
Milošević, Verica
Тип документа:
Чланак у часопису (Објављена верзија)
,
© 2015 Anatomical Society
Метаподаци
Приказ свих података о документуАпстракт:
Thyroid C-cells produce calcitonin (CT), a hypocalcemic hormone, that
acts as an inhibitor of bone resorption. In this study, we investigated
the effects of tamoxifen (TAM) as a selective estrogen receptor
modulator on thyroid C-cells, trabecular bone and biochemical markers of
bone metabolism in an animal model of androgen deficiency, represented
by middle-aged orchidectomized (Orx) rats. Fifteen-month-old male Wistar
rats were divided into: Orx and sham-operated (SO) groups. Rats from one
Orx group were injected subcutaneously with TAM citrate (Orx + TAM; 0.3
mgkg(-1) b.w.), while the rats from SO and a second Orx group received
vehicle alone, once a day for 3 weeks. The peroxidase-antiperoxidase
method was applied for localization of CT in C-cells. Thyroid C-cells
were morphometrically and ultrastructurally analyzed. An ImageJ
image-processing program was used to measure bone histomorphometric
parameters. Blood serum samples were analyzed for CT, osteocalcin (OC),
calcium (Ca2+) and phosphorus (P). Urinary Ca2+ concentrations were
measured. TAM treatment significantly increased thyroid C-cell volume
(V-c) and serum CT when compared with vehicle-treated Orx rats. Analysis
of trabecular microarchitecture of the tibia showed that administration
of TAM significantly increased cancellous bone area, trabecular
thickness and trabecular number, whereas trabecular separation was
significantly decreased compared with vehicle-treated Orx rats. Serum OC
and urinary Ca2+ concentrations were significantly lower in comparison
with the control Orx group. These results indicate that in our rat model
of androgen deficiency, TAM stimulated calcitonin-producing thyroid
C-cells and increased trabecular bone mass.
Кључне речи:
androgen deficiency; bone; calcitonin; rats; tamoxifen; thyroid C-cellsИзвор:
Journal of Anatomy, 2015, 226, 5, 489-496
DOI: 10.1111/joa.12298
ISSN: 0021-8782