Long-term intermittent feeding restores impaired GR signaling in the hippocampus of aged rat
2015
Аутори:
Tesic, VesnaPerovic, Milka
Lazic, Divna
Kojic, Snezana
Smiljanić, Kosara
Ruždijić, Sabera
Rakic, Ljubisav
Kanazir, Selma
Тип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
Diminished glucocorticoid signaling is associated with an age-related
decline in hippocampal functioning. In this study we demonstrate the
effect of intermittent, every other day (EOD) feeding on the
glucocorticoid hormone/glucocorticoid receptor (GR) system in the
hippocampus of middle-aged (18-month-old) and aged (24-month-old) Wistar
rats. In aged ad libitum-fed rats, a decrease in the level of total GR
and GR phosphorylated at Ser(232) (pGR) was detected. Conversely, aged
rats subjected to EOD feeding, starting from 6 months of age, showed an
increase in GR and pGR levels and a higher content of hippocampal
corticosterone. Furthermore, prominent nuclear staining of pGR was
observed in CM pyramidal and DG granule neurons of aged EOD-fed rats.
These changes were accompanied by increased Sglc-1 and decreased GFAP
transcription, pointing to upregulated transcriptional activity of GR.
EOD feeding also induced an increase in the expression of the
mineralocorticoid receptor. Our results reveal that intermittent feeding
restores impaired GR signaling in the hippocampus of aged animals by
inducing rather than by stabilizing GR signaling during aging. (C) 2015
Elsevier Ltd. All rights reserved.
Кључне речи:
Aging; Intermittent feeding; Dietary restriction; Hippocampus; Corticosterone; Glucocorticoid receptor isoformsИзвор:
Journal of Steroid Biochemistry and Molecular Biology, 2015, 149, 43-52
DOI: 10.1016/j.jsbmb.2015.01.013
ISSN: 0960-0760