Ex vivo effects of ibogaine on the activity of antioxidative enzymes in human erythrocytes
2015
Аутори:
Nikolić-Kokić, AleksandraOreščanin Dušić, Zorana
Spasojevic, Ivan
Slavic, Marija
Mijuskovic, Ana
Paskulin, Roman
Miljevic, Cedo
Spasić, Mihajlo
Blagojević, Duško
Тип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
Ethnopharmacological relevance: Ibogaine is a naturally occurring
alkaloid with psychotropic and metabotropic effects, derived from the
bark of the root of the West African Tabernanthe iboga plant. The tribes
of Kongo basin have been using iboga as a stimulant, for medicinal
purposes, and in rite of passage ceremonies, for centuries. Besides, it
has been found that this drug has anti-addictive effects.
Aim of the study: Previous studies have demonstrated that ibogaine
changed the quantity of ATP and energy related enzymes as well as the
activity of antioxidant enzymes in cells thus altering redox equilibrium
in a time manner. In this work, the mechanism of its action was further
studied by measuring the effects of ibogaine in human erythrocytes in
vitro on ATP liberation, membrane fluidity and antioxidant enzymes
activity.
Materials and methods: Heparinized human blood samples were incubated
with ibogaine (10 and 20 mu M) at 37 degrees C for 1 h. Blood plasma was
separated by centrifugation and the levels of ATP and uric acid were
measured 10 mm after the addition of ibogaine using standard kits. The
activity of copper zinc superoxide dismutase (SOD1), catalase (CAT),
glutathione peroxidase (GSH-Px) and glutathione reductase (GR) were
measured in erythrocytes after incubation period. The stability of SOD1
activity was further tested through in vitro incubation with H2O2 and
scanning of its electrophoretic profiles. Membrane fluidity was
determined using an electron paramagnetic resonance spin-labelling
method.
Results: Results showed that ibogaine treatment of erythrocytes in vitro
increased ATP concentration in the blood plasma without changes in
neither erythrocytes membrane fluidity nor uric acid concentration.
lbogaine also increased SOD1 activity in erythrocytes at both doses
applied here. Treatment with 20 mu M also elevated GR activity after in
vitro incubation at 37 degrees C. Electrophoretic profiles revealed that
incubation with ibogaine mitigates H2O2 mediated suppression of SOD1
activity.
Conclusion: Some of the effects of ibogaine seem to be mediated through
its influence on energy metabolism, redox active processes and the
effects of discrete fluctuations of individual reactive oxygen species
on different levels of enzyme activities. Overall, ibogaine acts as a
pro-antioxidant by increasing activity of antioxidative enzymes and as
an adaptagene in oxidative distress. (C) 2015 Published by Elsevier
Ireland Ltd.
Кључне речи:
Adaptogen; Aging; Alkaloids; Antioxidant; Metabolism; StressИзвор:
Journal of Ethnopharmacology, 2015, 164, 64-70
DOI: 10.1016/j.jep.2015.01.037
ISSN: 0378-8741