Antifungal activities of indigenous plant growth promoting Pseudomonas spp. from alfalfa and clover rhizosphere
2015
Аутори:
Josic, DraganaĆirić, Ana
Soković, Marina
Stanojkovic-Sebic, Aleksandra
Pivic, Radmila
Lepsanovic, Zorica
Glamočlija, Jasmina
Тип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
To select isolates suitable for use in biological control of pathogenic
fungi and as plant growth promoting (PGP) agents, Pseudomonas spp.,
indigenous to the rhizosphere of wild alfalfa and clover plants growing
on the old magmatic hill Vagan (Serbia), were isolated. Isolates were
selected on the basis of intrinsic antibiotic resistance (IAR) and PGP
traits: phosphate solubilization, production of siderophores, hydrogen
cyanide (HCN), acyl homoserine lactones (AHLs), indole acetic acid (IAA)
and enzymatic activities. The results of the genotyping by repetitive
sequence-based polymerase chain reaction, using ERIC and (GTG)(5)
primers, and phenotyping by IAR clustered the isolates in five groups,
represented by two isolates from alfalfa (Q1 and Q16) and three from
white clover (B25, B28 and B29). Isolates Q16 and B25 showed production
of HCN, IAA and AHLs. Good phosphate solubilization and production of
siderophores were observed on Q1 and Q16. The Q16 isolate exhibited high
enzymatic activity. The most promising PGP isolates, Q16 and B25, showed
the best antifungal activity against Trichoderma viride, and good
antifungal effect against Aspergillus fumigatus and Aspergillus niger,
while Penicillium verrucosum was the most resistant fungus. Pseudomonas
sp. B25 exhibited higher antifungal potential than Q16. The selected
isolates could be further investigated as biological control agents and
tested in field conditions to confirm their PGP efficacy.
Кључне речи:
rep-PCR; Pseudomonas; antifungal activityИзвор:
Frontiers in Life Science, 2015, 8, 2, 131-138
DOI: 10.1080/21553769.2014.998776
ISSN: 2155-3777