Osmotic swelling activates a novel anionic current with VRAC-like properties in a cytoplasmic droplet membrane from Phycomyces blakesleeanus sporangiophores
2015
Аутори:
Krizak, StrahinjaNikoic, Ljiljana
Stanic, Marina
Zizic, Milan
Zakrzewska, Joanna
Zivic, Miroslav
Todorović, Nataša
Тип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
We describe here whole-cell currents of droplets prepared from the
apical region of growing Phycomyces blakesleeanus sporangiophores.
Whole-cell current recordings revealed the osmotically activated,
outwardly rectifying, fast inactivating instantaneous current (ORIC)
with biophysical properties closely resembling volume-regulated anionic
current (VRAC). ORIC is activated under conditions of osmotically
induced swelling and shows strong selectivity for anions over cations.
In addition, ORIC shows voltage and time-dependent inactivation at
positive potentials and recovery from inactivation at negative
potentials. ORIC is blocked by anthracene-9-carboxylic acid, an anion
channel blocker, in a voltage-dependent manner. This is the first report
of the presence of VRAC-like current in an organism outside the chordate
lineage. (C) 2015 Institut Pasteur. Published by Elsevier Masson SAS.
All rights reserved.
Кључне речи:
Filamentous fungi; Whole-cell patch-clamp; Outward rectifier; Voltage-dependent inactivationИзвор:
Research in Microbiology, 2015, 166, 3, 162-173
DOI: 10.1016/j.resmic.2015.02.004
ISSN: 1769-7123