Upregulation of Nucleoside Triphosphate Diphosphohydrolase-1 and Ecto-5'-Nucleotidase in Rat Hippocampus after Repeated Low-Dose Dexamethasone Administration
2015
Аутори:
Drakulic, DunjaStanojlovic, Milos
Nedeljkovic, Nadezda
Grkovic, Ivana
Velickovic, Natasa
Gusevac, Ivana
Mitrovic, Natasa
Buzadzic, Ivana
Horvat, Anica
Тип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
Although dexamethasone (DEX), a synthetic glucocorticoid receptor (GR)
analog with profound effects on energy metabolism, immune system, and
hypothalamic-pituitary-adrenal axis, is widely used therapeutically, its
impact on the brain is poorly understood. The aim of the present study
was to explore the effect of repeated low-dose DEX administration on the
activity and expression of the ectonucleotidase enzymes which hydrolyze
and therefore control extracellular ATP and adenosine concentrations in
the synaptic cleft. Ectonucleotidases tested were ectonucleoside
triphosphate diphosphohydrolase 1-3 (NTPDase1-3) and
ecto-5'-nucleotidase (eN), whereas the effects were evaluated in two
brain areas that show different sensitivity to glucocorticoid action,
hippocampus, and cerebral cortex. In the hippocampus, but not in
cerebral cortex, modest level of neurodegenerative changes as well as
increase in ATP, ADP, and AMP hydrolysis and upregulation of NTPDase1
and eN mRNA expression ensued under the influence of DEX. The observed
pattern of ectonucleotidase activation, which creates tissue volume with
enhanced capacity for adenosine formation, is the hallmark of the
response after different insults to the brain.
Кључне речи:
Dexamethasone; Ectonucleotidases; Synaptic membranes; HippocampusИзвор:
Journal of Molecular Neuroscience, 2015, 55, 4, 959-967
DOI: 10.1007/s12031-014-0452-y
ISSN: 1559-1166