Cadmium administration affects circulatory mononuclear cells in rats
2015
Autori:
Djokic, JelenaPopov Aleksandrov, Aleksandra
Ninkov, Marina
Mirkov, Ivana
Zolotarevski, Lidija
Kataranovski, Dragan S.
Kataranovski, Milena
Tip dokumenta:
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt:
Although numerous investigations have demonstrated a direct effect of
cadmium (Cd) on peripheral blood mononuclear cell (PBMC) activity in
humans, there is virtually no data concerning the in vivo impact of this
metal on circulatory mononuclear cells. In this study, the effects of a
sub-lethal Cd (1 mg/kg) dose were examined in rats 48 h following a
single intraperitoneal injection. Cd treatment resulted in increased
total peripheral blood leukocyte levels; however, decreases in PBMC
numbers were seen. These changes coincided with an accumulation of
mononuclear cells in the lungs and an increase in mononuclear cells
expressing CD11b. A lack of effect of Cd on spontaneous nitric oxide
(NO) production and on iNOS mRNA levels in the PBMC was also noted.
Differential effects of Cd on PBMC inflammatory cytokine (IL-1 beta, TNF
alpha, IL-6, IFN gamma, and IL-17) gene expression and production were
also seen. Specifically, except for IL-1 beta (levels increased), there
were decreases (relative to controls) in mRNA levels for all the other
cytokines examined. While there were no Cd treatment-related changes in
spontaneous production of the cytokines assessed, there seemed to be a
trend (p = 0.06) toward a decrease in spontaneous IL-6 release. When
these harvested cells were stimulated ex vivo, there was no effect from
Cd exposure on LPS-stimulated IL-1 beta and TNF alpha or on
ConA-stimulated IFN gamma or IL-17 production, but a decrease in IL-6
production in response to LPS was, again, noted. A preliminary study
with a lower Cd dose (0.5 mg/kg) revealed some of the same outcomes
noted here (mononuclear cell infiltration into lungs, increases in PBMC
IL-1 beta mRNA levels), but differential (increased IL-17 mRNA levels)
or newly detected outcomes (increased levels of IL-1 alpha mRNA) as
well. The described effects of the single in vivo exposure to Cd on PBMC
might contribute to a better overall understanding of the
immunomodulatory potential of this environmental contaminant.
Ključne reči:
CD11b; Inflammatory cytokines; intraperitoneal cadmium; rats; peripheral blood mononuclear cellsIzvor:
Journal Of Immunotoxicology, 2015, 12, 2, 115-123
DOI: 10.3109/1547691X.2014.904955
ISSN: 1547-6901