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Methanolic extract of Origanum vulgare ameliorates type 1 diabetes through antioxidant, anti-inflammatory and anti-apoptotic activity
dc.creator | Vujičić, Milica | |
dc.creator | Nikolić, Ivana | |
dc.creator | Kontogianni, Vassiliki G. | |
dc.creator | Saksida, Tamara | |
dc.creator | Charisiadis, Pantelis | |
dc.creator | Oreščanin Dušić, Zorana | |
dc.creator | Blagojević, Duško | |
dc.creator | Stošić-Grujičić, Stanislava | |
dc.creator | Tzakos, Andreas G. | |
dc.creator | Stojanović, Ivana D. | |
dc.date.accessioned | 2016-05-23T10:59:51Z | |
dc.date.issued | 2015 | |
dc.identifier.issn | 1475-2662 | |
dc.identifier.uri | https://radar.ibiss.bg.ac.rs/handle/123456789/1988 | |
dc.description.abstract | Type 1 diabetes (T1D), an autoimmune inflammatory disorder, develops as a consequence of pancreatic beta-cell destruction and results in hyperglycaemia. Since current T1D therapy mainly involves insulin replacement, the aim of the present study was to evaluate the therapeutic potential of Origanum vulgare L. ssp. hirtum (Greek oregano) leaf extract rich in biophenols for the treatment of T1D. The phytochemical profile of methanolic oregano extract (MOE) and aqueous oregano extract (AOE) was determined by liquid chromatography/electrospray ion-trap tandem MS (LC/DAD/ESI-MSn), while their main compounds were quantified by HPLC with diode array detection. After establishing their potent in vitro antioxidant activity, the extracts were administered to C57BL/6 mice treated with multiple low doses of streptozotocin for diabetes induction. While prophylactic AOE therapy had no impact on diabetes induction, MOE reduced diabetes incidence and preserved normal insulin secretion. In addition, MOE scavenged reactive oxygen and nitrogen species and, therefore, alleviated the need for the up-regulation of antioxidant enzymes. MOE treatment specifically attenuated the pro-inflammatory response mediated by T helper 17 cells and enhanced anti-inflammatory T helper 2 and T regulatory cells through the impact on specific signalling pathways and transcription factors. Importantly, MOE preserved beta-cells from in vitro apoptosis via blockade of caspase 3. Finally, rosmarinic acid, a predominant compound in MOE, exhibited only partial protection from diabetes induction. In conclusion, acting as an antioxidant, immunomodulator and in an anti-apoptotic manner, MOE protected mice from diabetes development. Seemingly, there is more than one compound responsible for the beneficial effect of MOE. | en |
dc.description.sponsorship | Ministry of Education, Science and Technological Development, Republic of Serbia {[}173013]; European Union; Greek National Funds Through the Operational Program `THESSALY-MAINLAND GREECE AND EPIRUS' of the National Strategic Reference Framework (NSRF); Greek national funds through the Operational Program `Education and Lifelong Learning' of the National Strategic Reference Framework (NSRF)-Research Funding Program: ARISTEIA II, Investing in knowledge society through the European Social Fund | |
dc.language | English | |
dc.rights | restrictedAccess | |
dc.source | British Journal of Nutrition | |
dc.subject | Type 1 diabetes | |
dc.subject | Oregano | |
dc.subject | T helper 2 cells | |
dc.subject | T regulatory cells | |
dc.title | Methanolic extract of Origanum vulgare ameliorates type 1 diabetes through antioxidant, anti-inflammatory and anti-apoptotic activity | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Тзакос, Aндреас Г.; Вујичић, Милица; Благојевић, Душко П.; Стошић-Грујичић, Станислава Д.; Орешчанин Душић, Зорана; Николић, Ивана; Контогианни, Вассилики Г.; Саксида, Тамара; Стојановиц, Ивана; Цхарисиадис, Пантелис; | |
dc.citation.issue | 5 | |
dc.citation.volume | 113 | |
dc.identifier.doi | 10.1017/S0007114514004048 | |
dc.identifier.scopus | 2-s2.0-84926418133 | |
dc.identifier.wos | 000352199200007 | |
dc.citation.spage | 770 | |
dc.citation.epage | 782 | |
dc.type.version | publishedVersion | en |
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