Effect of sucrose on shoot regeneration in Agrobacterium transformed Hypericum perforatum L. roots
2015
Аутори:
Vinterhalter, BrankaZdravković-Korać, Snežana
Banjac, Nevena
Bohanec, Borut
Vinterhalter, Dragan
Savić, Jelena
Тип документа:
Чланак у часопису (Објављена верзија)
,
© 2015, Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków
Метаподаци
Приказ свих података о документуАпстракт:
The aim of the present study was to investigate the effect of sucrose on
shoot regeneration potential in Hypericum perforatum L. roots obtained
by Agrobacterium rhizogenes transformation. The morphological evaluation
of transgenic roots grown on media supplemented with sucrose (0.5, 1, 2,
4, 6 and 8 \%) indicated that both genotype and sucrose concentration
significantly affected root elongation and branching, as well as shoot
regeneration. For two of five analyzed clones, lower sucrose
concentrations (up to 2 \%) led to intensive shoot regeneration, while
the other three clones intensified shoot development only at elevated
sucrose concentrations (4 \%). For all clones, concentrations above 4 \%
had a deleterious effect on both root and shoot development. Genetic
characterization of regenerated shoots revealed that all tested clones
were diploid with an average of 0.670 +/- 0.002 pg of DNA per nucleus,
with no significant differences between transgenic and non-transformed
plants and, according to PCR, with integrated A. rhizogenes rolA, -B, -C
and -D genes. Real-time RT-PCR confirmed the expression of rolA, -B and
-C, while expression of the rolD gene was not detected. Differences were
detected in the absolute amounts of transcripts between analyzed clones,
with the highest levels of expression for all three analyzed rol genes
in a clone previously defined as having high root differentiation and
less effective shoot regeneration potential. The observed variations in
morphogenesis potential could be attributed to different levels of
expression of integrated rolA, -B and -Cgenes; while sucrose
additionally pointed out these trends.
Кључне речи:
Hypericum perforatum; In vitro; Shoot regeneration; Sucrose; Agrobacterium rhizogenes; rol genesИзвор:
Acta Physiologiae Plantarum, 2015, 37, 37-Повезане информације:
- Повезани садржај
https://radar.ibiss.bg.ac.rs/handle/123456789/2984
DOI: 10.1007/s11738-015-1785-z
ISSN: 0137-5881