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Pharmacological inhibition of MIF interferes with trophoblast cell migration and invasiveness
dc.creator | Jovanovic Krivokuca, M. | |
dc.creator | Stefanoska, I. | |
dc.creator | Abu Rabi, T. | |
dc.creator | Al-Abed, Y. | |
dc.creator | Stošić-Grujičić, Stanislava | |
dc.creator | Vicovac, Lj | |
dc.date.accessioned | 2016-05-23T10:59:54Z | |
dc.date.issued | 2015 | |
dc.identifier.issn | 1532-3102 | |
dc.identifier.uri | https://radar.ibiss.bg.ac.rs/handle/123456789/2009 | |
dc.description.abstract | Introduction: Macrophage migration inhibitory factor (MIF) is expressed by villous and extravillous cytotrophoblast. This study was aimed to investigate functional relevance of MIF for human trophoblast. Methods: MIF mRNA and protein were documented in cytotrophoblast (CT) and extravillous trophoblast cell line HTR-8/SVneo by RT-PCR, Western blot (WB), and immunocytochemistry. Recombinant human MIF (rhMIF), or its specific inhibitor (S,R)-3-(4-hydroxypheny1)-4,5-dihydro-5-isoxazole acetic acid methyl ester (ISO-1) were used in Wound healing migration and Matrigel invasion tests. Potential effectors, integrin subunits and matrix metalloproteinases (MMP) were studied using WB and gelatin zymography, respectively. Results: Blocking endogenous MIF by ISO-1 decreased HTR-8/SVneo cell migration dose dependently, most significantly with 200 mu g/ml to 65\% of control. Supplementation with rhMIF induced a significant stimulation to 129\% of control with 200 ng/ml. In CT cell invasion test, ISO-1 at 200 mu g/ml reduced invasion to 59\% of control, while rhMIF (200 ng/m1) induced stimulation to 159\% of control. In HTR-8/SVneo cells, invasion was significantly inhibited by ISO-1 to 40\%, and increased to 150\% of control by rhMIF (200 ng/ml). Integrin alpha 1 was reduced by ISO-1 in both cell types, while integrins alpha 5 and beta 1 were not changed. Addition of rhMIF increased integrin alpha 1. In the presence of ISO-1, levels of MMP-2 and MMP-9 were reduced in CT and HTR-8/SVneo, while rhMIF stimulated MMP-2 in CT and MMP-9 in HTR-8/SVneo cells. Conclusion: Reported findings provide the first insight into the cellular effects of MIF in human trophoblast, which acts to promote cell migration and invasion. (C) 2014 Elsevier Ltd. All rights reserved. | en |
dc.description.sponsorship | Ministry of Education, Science and Technological Development, Republic of Serbia {[}173004]; {[}173013] | |
dc.language | English | |
dc.rights | restrictedAccess | |
dc.source | Placenta | |
dc.subject | Placenta | |
dc.subject | Trophoblast | |
dc.subject | Invasion | |
dc.title | Pharmacological inhibition of MIF interferes with trophoblast cell migration and invasiveness | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Вицовац, Љ; Кривокуца, М. Јовановиц; Aбу Раби, Т.; Стошић-Грујичић, Станислава Д.; Aл-Aбед, Y.; Стефаноска, И.; | |
dc.citation.issue | 2 | |
dc.citation.volume | 36 | |
dc.identifier.doi | 10.1016/j.placenta.2014.12.003 | |
dc.identifier.scopus | 2-s2.0-84920983356 | |
dc.identifier.wos | 000349583100008 | |
dc.citation.spage | 150 | |
dc.citation.epage | 159 | |
dc.type.version | publishedVersion | en |
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