Long-term dietary restriction differentially affects the expression of BDNF and its receptors in the cortex and hippocampus of middle-aged and aged male rats
2015
Аутори:
Smiljanić, KosaraPesic, Vesna
Mladenović, Aleksandra
Pavković, Željko
Brkić, Marjana
Ruždijić, Sabera
Kanazir, Selma
Тип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
Dietary restriction (DR) exerts significant beneficial effects in terms
of aging and age-related diseases in many organisms including humans.
The present study aimed to examine the influence of long-term DR on the
BDNF system at the transcriptional and translational levels in the
cortex and hippocampus of middle-aged (12-month-old) and aged
(24-month-old) male Wistar rats. The obtained results revealed that the
DR upregulated the expression of exon-specific BDNF transcripts in both
regions, followed by elevated levels of mBDNF only in the cortex in
middle-aged animals. In aged animals, DR modulated BDNF protein levels
by increasing proBDNF and by declining mBDNF levels. Additionally,
elevated levels of the full-length TrkB accompanied by a decreased level
of the less-glycosylated TrkB protein were observed in middle-aged rats
following DR, while in aged rats, DR amplified only the expression of
the less-glycosylated form of TrkB. The levels of phosphorylated
TrkB(Y816) were stable during aging regardless of feeding. Reduced
levels of p75(NTR) were detected in both regions of middle-aged DR-fed
animals, while a significant increase was measured in the cortex of aged
DR-fed rats. These findings shed additional light on DR as a modulator
of BDNF system revealing its disparate effects in middle-aged and aged
animals. Given the importance of the proBDNF/BDNF circuit-level
expression in different brain functions and various aspects of behavior,
it is necessary to further elucidate the optimal duration of the applied
dietary regimen with regard to the animal age in order to achieve its
most favorable effects.
Кључне речи:
TrkB; Dietary restriction; Brain; AgingИзвор:
Biogerontology, 2015, 16, 1, 71-83
DOI: 10.1007/s10522-014-9537-9
ISSN: 1573-6768