Effects of different culture conditions on biological potential and metabolites production in three Penicillium isolates
2015
Аутори:
Reis, Filipa S.Ćirić, Ana
Stojković, Dejan
Barros, Lillian
Ljaljevic-Grbic, Milica
Soković, Marina
Ferreira, Isabel C. F. R.
Тип документа:
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт:
The genus Penicillium is well known for its importance in drug and food
production. Certain species are produced on an industrial scale for the
production of antibiotics (e.g. penicillin) or for insertion in food
(e.g. cheese). In the present work, three Penicillium species, part of
the natural mycobiota growing on various food products were selected -
P. ochrochloron, P. funiculosum and P. verrucosum var. cyclopium. The
objective of our study was to value these species from the point of view
of production of bioactive metabolites. The species were obtained after
inoculation and growth in Czapek and Malt media. Both mycelia and
culture media were analyzed to monitor the production of different
metabolites by each fungus and their release to the culture medium. The
concentrations of sugars, organic acids, phenolic acids and tocopherols
were determined. Antioxidant activity of the phenolic extracts was
evaluated, as also the antimicrobial activity of phenolic acids, organic
acids and tocopherols extracts. Rhamnose, xylose, fructose and trehalose
were found in all the mycelia and culture media; the prevailing organic
acids were oxalic and fumaric acids, and protocatechuic and
p-hydroxybenzoic acids were the most common phenolic acids; g-tocopherol
was the most abundant vitamin E isoform. Generally, the phenolic
extracts corresponding to the mycelia samples revealed higher
antioxidant activity. Concerning the antimicrobial activity there were
some fluctuations, however all the studied species revealed activity
against the tested strains. Therefore, the in-vitro bioprocesses can be
an alternative for the production of bioactive metabolites that can be
used by pharmaceutical industry.
Кључне речи:
Antimicrobial activity; antioxidant; fungi growth; metabolites; PenicilliumИзвор:
Drug Development and Industrial Pharmacy, 2015, 41, 2, 253-262
DOI: 10.3109/03639045.2013.858738
ISSN: 1520-5762